Bonding antimicrobial rhamnolipids onto medical grade PDMS
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/150713 |
Resumo: | Funding Information: Portugal 2020 for the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa RNEM ) is also acknowledged. The authors also acknowledge the Designer Nuno Monge for the artwork, Isabel Dias Nogueira (Microlab, Instituto Superior Técnico, Lisbon University) for the technical assistance with the SEM analysis, Andreia Bento for the assistance with the UHPLC-MS equipment (iMed.Ulisboa) and Professor Maria José Ferreira (FFULisboa) for allowing the use of the automatic flash chromatography equipment. Publisher Copyright: © 2022 |
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Bonding antimicrobial rhamnolipids onto medical grade PDMSA strategy to overcome multispecies vascular catheter-related infectionsAntibiofilmAntimicrobialsBiosurfactantsCandida albicansMedical devicesPDMSRhamnolipidsStaphylococcus aureusStaphylococcus epidermidisBiotechnologySurfaces and InterfacesPhysical and Theoretical ChemistryColloid and Surface ChemistryFunding Information: Portugal 2020 for the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa RNEM ) is also acknowledged. The authors also acknowledge the Designer Nuno Monge for the artwork, Isabel Dias Nogueira (Microlab, Instituto Superior Técnico, Lisbon University) for the technical assistance with the SEM analysis, Andreia Bento for the assistance with the UHPLC-MS equipment (iMed.Ulisboa) and Professor Maria José Ferreira (FFULisboa) for allowing the use of the automatic flash chromatography equipment. Publisher Copyright: © 2022In clinic there is a demand to solve the drawback of medical devices multispecies related infections. Consequently, different biomaterial surfaces, such as vascular catheters, urgently need improvement regarding their antifouling/antimicrobial properties. In this work, we covalently functionalized medical grade polydimethylsiloxane (PDMS) with antimicrobial rhamnolipids to investigate the biomaterial surface activity towards mono and dual species biofilms. Preparation of surfaces with “piranha” oxidation, followed by APTES bonding and carbodiimide reaction with rhamnolipids effectively bonded these compounds to PDMS surface as confirmed by FTIR-ATR and XPS analysis. Generated surfaces were active towards S. aureus biofilm formation showing a 4.2 log reduction while with S. epidermidis and C. albicans biofilms a reduction of 1.2 and 1.0 log reduction, respectively, was observed. Regarding dual-species testing the higher biofilm log reduction observed was 1.9. Additionally, biocompatibility was assessed by cytocompatibility towards human fibroblastic cells, low platelet activation and absence of vascular irritation. Our work not only sheds light on using covalently bonded rhamnolipids towards dual species biofilms but also highlights the biocompatibility of the obtained PDMS surfaces.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)UNINOVA-Instituto de Desenvolvimento de Novas TecnologiasDCM - Departamento de Ciência dos MateriaisRUNDardouri, MaïssaAljnadi, Israa M.Deuermeier, JonasSantos, CatarinaCosta, FabiolaMartin, VictorFernandes, Maria H.Gonçalves, LídiaBettencourt, AnaGomes, Pedro SousaRibeiro, Isabel A. C.2023-03-16T22:36:46Z2022-092022-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/150713eng0927-7765PURE: 56072652https://doi.org/10.1016/j.colsurfb.2022.112679info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:33:01Zoai:run.unl.pt:10362/150713Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:16.309101Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Bonding antimicrobial rhamnolipids onto medical grade PDMS A strategy to overcome multispecies vascular catheter-related infections |
| title |
Bonding antimicrobial rhamnolipids onto medical grade PDMS |
| spellingShingle |
Bonding antimicrobial rhamnolipids onto medical grade PDMS Dardouri, Maïssa Antibiofilm Antimicrobials Biosurfactants Candida albicans Medical devices PDMS Rhamnolipids Staphylococcus aureus Staphylococcus epidermidis Biotechnology Surfaces and Interfaces Physical and Theoretical Chemistry Colloid and Surface Chemistry |
| title_short |
Bonding antimicrobial rhamnolipids onto medical grade PDMS |
| title_full |
Bonding antimicrobial rhamnolipids onto medical grade PDMS |
| title_fullStr |
Bonding antimicrobial rhamnolipids onto medical grade PDMS |
| title_full_unstemmed |
Bonding antimicrobial rhamnolipids onto medical grade PDMS |
| title_sort |
Bonding antimicrobial rhamnolipids onto medical grade PDMS |
| author |
Dardouri, Maïssa |
| author_facet |
Dardouri, Maïssa Aljnadi, Israa M. Deuermeier, Jonas Santos, Catarina Costa, Fabiola Martin, Victor Fernandes, Maria H. Gonçalves, Lídia Bettencourt, Ana Gomes, Pedro Sousa Ribeiro, Isabel A. C. |
| author_role |
author |
| author2 |
Aljnadi, Israa M. Deuermeier, Jonas Santos, Catarina Costa, Fabiola Martin, Victor Fernandes, Maria H. Gonçalves, Lídia Bettencourt, Ana Gomes, Pedro Sousa Ribeiro, Isabel A. C. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias DCM - Departamento de Ciência dos Materiais RUN |
| dc.contributor.author.fl_str_mv |
Dardouri, Maïssa Aljnadi, Israa M. Deuermeier, Jonas Santos, Catarina Costa, Fabiola Martin, Victor Fernandes, Maria H. Gonçalves, Lídia Bettencourt, Ana Gomes, Pedro Sousa Ribeiro, Isabel A. C. |
| dc.subject.por.fl_str_mv |
Antibiofilm Antimicrobials Biosurfactants Candida albicans Medical devices PDMS Rhamnolipids Staphylococcus aureus Staphylococcus epidermidis Biotechnology Surfaces and Interfaces Physical and Theoretical Chemistry Colloid and Surface Chemistry |
| topic |
Antibiofilm Antimicrobials Biosurfactants Candida albicans Medical devices PDMS Rhamnolipids Staphylococcus aureus Staphylococcus epidermidis Biotechnology Surfaces and Interfaces Physical and Theoretical Chemistry Colloid and Surface Chemistry |
| description |
Funding Information: Portugal 2020 for the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa RNEM ) is also acknowledged. The authors also acknowledge the Designer Nuno Monge for the artwork, Isabel Dias Nogueira (Microlab, Instituto Superior Técnico, Lisbon University) for the technical assistance with the SEM analysis, Andreia Bento for the assistance with the UHPLC-MS equipment (iMed.Ulisboa) and Professor Maria José Ferreira (FFULisboa) for allowing the use of the automatic flash chromatography equipment. Publisher Copyright: © 2022 |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-09 2022-09-01T00:00:00Z 2023-03-16T22:36:46Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/150713 |
| url |
http://hdl.handle.net/10362/150713 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0927-7765 PURE: 56072652 https://doi.org/10.1016/j.colsurfb.2022.112679 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
11 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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