Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood

Detalhes bibliográficos
Autor(a) principal: Amorim, Manuela
Data de Publicação: 2018
Outros Autores: Bravo, Joana, Silva, Ana Isabel, Alves, Cecí­lia Juliana, Monteiro, Pedro, Magalhãess, Ana, Summavielle, Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/16251
Resumo: Adolescent development of the prefrontal cortex (PFC) is accompanied by important changes in glutamatergic, GABAergic and dopaminergic circuitries, susceptible to modulation by N-methyl-D-aspartate receptors (NMDAR) antagonists. Repeated ketamine was associated with social and memory deficits, but other relevant factors, such as anxiety, were not sufficiently addressed. The present study aimed to examine the behavioral and molecular consequences of repeated exposure to ketamine with a particular focus in anxiety. Methods. We treated male adolescent Wistar rats, starting postnatal day (PND) 35, with ketamine (30 mg/kg, i.p, 7 days). Behavioral evaluation was conducted in the adulthood (PND 60). The elevated plus maze (EPM) and open field tests were used to evaluate anxiety and locomotion, while sociability and novelty recognition were assessed through the novel object recognition (NOR) and the sociability and social novelty tests. At the end of the behavioral evaluation, brains were dissected and the prefrontal cortex used for biochemical evaluation. Results. Analysis of the elevated plus maze (EPM) data revealed a ketamine-induced anxiety-like profile, corroborated by the open field data. Ketaminetreated rats also failed to increase contact time with a conspecific in the social affiliation test and with an unknown rat in the novelty preference test. At the molecular level, frontal expression levels of tyrosine hydroxylase were found decreased. Conclusion. Altogether, these results show that repeated ketamine-exposure in the adolescent may result in long-term anxiety
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spelling Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthoodKetamineNMDARAdolescentAnxietySchizophreniaPrefrontal cortexAdolescent development of the prefrontal cortex (PFC) is accompanied by important changes in glutamatergic, GABAergic and dopaminergic circuitries, susceptible to modulation by N-methyl-D-aspartate receptors (NMDAR) antagonists. Repeated ketamine was associated with social and memory deficits, but other relevant factors, such as anxiety, were not sufficiently addressed. The present study aimed to examine the behavioral and molecular consequences of repeated exposure to ketamine with a particular focus in anxiety. Methods. We treated male adolescent Wistar rats, starting postnatal day (PND) 35, with ketamine (30 mg/kg, i.p, 7 days). Behavioral evaluation was conducted in the adulthood (PND 60). The elevated plus maze (EPM) and open field tests were used to evaluate anxiety and locomotion, while sociability and novelty recognition were assessed through the novel object recognition (NOR) and the sociability and social novelty tests. At the end of the behavioral evaluation, brains were dissected and the prefrontal cortex used for biochemical evaluation. Results. Analysis of the elevated plus maze (EPM) data revealed a ketamine-induced anxiety-like profile, corroborated by the open field data. Ketaminetreated rats also failed to increase contact time with a conspecific in the social affiliation test and with an unknown rat in the novelty preference test. At the molecular level, frontal expression levels of tyrosine hydroxylase were found decreased. Conclusion. Altogether, these results show that repeated ketamine-exposure in the adolescent may result in long-term anxietyAshdin PublishingRepositório Científico do Instituto Politécnico do PortoAmorim, ManuelaBravo, JoanaSilva, Ana IsabelAlves, Cecí­lia JulianaMonteiro, PedroMagalhãess, AnaSummavielle, Teresa2020-09-14T10:54:55Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/16251engAmorim, M., Bravo, J., Silva, A. I., Alves, C. J., Monteiro, P., Magalhãess, A., & Summavielle, T. (2018). Repeated Exposure to Ketamine in Adolescent Rats Results in Persistent Anxiety in the Adulthood. Journal of Drug and Alcohol Research, 07(01). https://doi.org/10.4303/jdar/23606010.4303/jdar/236060info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T01:54:24Zoai:recipp.ipp.pt:10400.22/16251Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:35:56.782871Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
title Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
spellingShingle Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
Amorim, Manuela
Ketamine
NMDAR
Adolescent
Anxiety
Schizophrenia
Prefrontal cortex
title_short Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
title_full Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
title_fullStr Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
title_full_unstemmed Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
title_sort Repeated exposure to ketamine in adolescent rats results in persistent anxiety in the adulthood
author Amorim, Manuela
author_facet Amorim, Manuela
Bravo, Joana
Silva, Ana Isabel
Alves, Cecí­lia Juliana
Monteiro, Pedro
Magalhãess, Ana
Summavielle, Teresa
author_role author
author2 Bravo, Joana
Silva, Ana Isabel
Alves, Cecí­lia Juliana
Monteiro, Pedro
Magalhãess, Ana
Summavielle, Teresa
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Amorim, Manuela
Bravo, Joana
Silva, Ana Isabel
Alves, Cecí­lia Juliana
Monteiro, Pedro
Magalhãess, Ana
Summavielle, Teresa
dc.subject.por.fl_str_mv Ketamine
NMDAR
Adolescent
Anxiety
Schizophrenia
Prefrontal cortex
topic Ketamine
NMDAR
Adolescent
Anxiety
Schizophrenia
Prefrontal cortex
description Adolescent development of the prefrontal cortex (PFC) is accompanied by important changes in glutamatergic, GABAergic and dopaminergic circuitries, susceptible to modulation by N-methyl-D-aspartate receptors (NMDAR) antagonists. Repeated ketamine was associated with social and memory deficits, but other relevant factors, such as anxiety, were not sufficiently addressed. The present study aimed to examine the behavioral and molecular consequences of repeated exposure to ketamine with a particular focus in anxiety. Methods. We treated male adolescent Wistar rats, starting postnatal day (PND) 35, with ketamine (30 mg/kg, i.p, 7 days). Behavioral evaluation was conducted in the adulthood (PND 60). The elevated plus maze (EPM) and open field tests were used to evaluate anxiety and locomotion, while sociability and novelty recognition were assessed through the novel object recognition (NOR) and the sociability and social novelty tests. At the end of the behavioral evaluation, brains were dissected and the prefrontal cortex used for biochemical evaluation. Results. Analysis of the elevated plus maze (EPM) data revealed a ketamine-induced anxiety-like profile, corroborated by the open field data. Ketaminetreated rats also failed to increase contact time with a conspecific in the social affiliation test and with an unknown rat in the novelty preference test. At the molecular level, frontal expression levels of tyrosine hydroxylase were found decreased. Conclusion. Altogether, these results show that repeated ketamine-exposure in the adolescent may result in long-term anxiety
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2020-09-14T10:54:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/16251
url http://hdl.handle.net/10400.22/16251
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amorim, M., Bravo, J., Silva, A. I., Alves, C. J., Monteiro, P., Magalhãess, A., & Summavielle, T. (2018). Repeated Exposure to Ketamine in Adolescent Rats Results in Persistent Anxiety in the Adulthood. Journal of Drug and Alcohol Research, 07(01). https://doi.org/10.4303/jdar/236060
10.4303/jdar/236060
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Ashdin Publishing
publisher.none.fl_str_mv Ashdin Publishing
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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