Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.5/22435 |
Resumo: | Research Areas: Veterinary Sciences |
| id |
RCAP_bc8ee9dbe5f7575e652bb73e0a58d36b |
|---|---|
| oai_identifier_str |
oai:www.repository.utl.pt:10400.5/22435 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatmentCanine leishmaniosisperipheral blood mononuclear cellslymph nodebone marrowcytokine gene expressionmeglumine antimoniatemiltefosineallopurinolResearch Areas: Veterinary SciencesCanine leishmaniosis (CanL) caused by Leishmania infantum is a zoonotic disease of global concern. Antileishmanial drug therapies commonly used to treat sick dogs improve their clinical condition, although when discontinued relapses can occur. Thus, the current study aims to evaluate the effect of CanL treatments in peripheral blood, lymph node, and bone marrow cytokine profile associated with clinical recovery. Two groups of six dogs diagnosed with CanL were treated with miltefosine combined with allopurinol and meglumine antimoniate combined with allopurinol (MT+A and MG+A), respectively. At diagnosis and after treatment, during a 3-month follow-up, clinical signs, hematological and biochemical parameters, urinalysis results and antileishmanial antibody titers were registered. Furthermore, peripheral blood, popliteal lymph node, and bone marrow samples were collected to assess the gene expression of IL-2, IL-4, IL-5, IL-10, IL-12, TNF-alpha, TGF-beta, and IFN-gamma by qPCR. In parallel, were also evaluated samples obtained from five healthy dogs. Both treatment protocols promoted the remission of clinical signs as well as normalization of hematological and biochemical parameters and urinalysis values. Antileishmanial antibodies returned to non-significant titers in all dogs. Sick dogs showed a generalized upregulation of IFN-gamma and downregulation of IL-2, IL-4, and TGF-beta, while gene expression of IL-12, TNF-alpha, IL-5, and IL-10 varied between groups and according to evaluated tissue. A trend to the normalization of cytokine gene expression was induced by both miltefosine and meglumine antimoniate combined therapies. However, IFN-gamma gene expression was still up-regulated in the three evaluated tissues. Furthermore, the effect of treatment in the gene expression of cytokines that were not significantly changed by infection, indicates that miltefosine and meglumine antimoniate combined therapy directly affects cytokine generation. Both combined therapies are effective in CanL treatment, leading to sustained pro-inflammatory immune environments that can compromise parasite survival and favor dogs' clinical cure. In the current study, anti-inflammatory and regulatory cytokines do not seem to play a prominent role in CanL or during clinical recovery.Dirk Werling, Royal Veterinary College (RVC), United KingdomRepositório da Universidade de LisboaSantos, Marcos André FerreiraAlexandre-Pires, GraçaPereira, Maria A.Marques, Cátia Filipa SaraivaGomes, JoanaCorreia, Jorge Manuel de JesusDuarte, AnaGomes, LídiaRodrigues, ArmandaBasso, AlexandraReisinho, AnaMeireles, JoséSantos-Mateus, DavidVilla Brito, Maria TeresaTavares, LuisSantos-Gomes, Gabriela M.Pereira da Fonseca, Isabel2021-10-27T22:33:33Z2019-10-182019-10-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22435engSantos MF, Alexandre-Pires G, Pereira MA, Marques CS, Gomes J, Correia J, Duarte A, Gomes L, Rodrigues AV, Basso A, et al. 2019. Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment. Frontiers in Veterinary Sciences. 6:36210.3389/fvets.2019.00362Frontiers in Veterinary Sciencesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:57Zoai:www.repository.utl.pt:10400.5/22435Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:51.719592Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment |
| title |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment |
| spellingShingle |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment Santos, Marcos André Ferreira Canine leishmaniosis peripheral blood mononuclear cells lymph node bone marrow cytokine gene expression meglumine antimoniate miltefosine allopurinol |
| title_short |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment |
| title_full |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment |
| title_fullStr |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment |
| title_full_unstemmed |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment |
| title_sort |
Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment |
| author |
Santos, Marcos André Ferreira |
| author_facet |
Santos, Marcos André Ferreira Alexandre-Pires, Graça Pereira, Maria A. Marques, Cátia Filipa Saraiva Gomes, Joana Correia, Jorge Manuel de Jesus Duarte, Ana Gomes, Lídia Rodrigues, Armanda Basso, Alexandra Reisinho, Ana Meireles, José Santos-Mateus, David Villa Brito, Maria Teresa Tavares, Luis Santos-Gomes, Gabriela M. Pereira da Fonseca, Isabel |
| author_role |
author |
| author2 |
Alexandre-Pires, Graça Pereira, Maria A. Marques, Cátia Filipa Saraiva Gomes, Joana Correia, Jorge Manuel de Jesus Duarte, Ana Gomes, Lídia Rodrigues, Armanda Basso, Alexandra Reisinho, Ana Meireles, José Santos-Mateus, David Villa Brito, Maria Teresa Tavares, Luis Santos-Gomes, Gabriela M. Pereira da Fonseca, Isabel |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Santos, Marcos André Ferreira Alexandre-Pires, Graça Pereira, Maria A. Marques, Cátia Filipa Saraiva Gomes, Joana Correia, Jorge Manuel de Jesus Duarte, Ana Gomes, Lídia Rodrigues, Armanda Basso, Alexandra Reisinho, Ana Meireles, José Santos-Mateus, David Villa Brito, Maria Teresa Tavares, Luis Santos-Gomes, Gabriela M. Pereira da Fonseca, Isabel |
| dc.subject.por.fl_str_mv |
Canine leishmaniosis peripheral blood mononuclear cells lymph node bone marrow cytokine gene expression meglumine antimoniate miltefosine allopurinol |
| topic |
Canine leishmaniosis peripheral blood mononuclear cells lymph node bone marrow cytokine gene expression meglumine antimoniate miltefosine allopurinol |
| description |
Research Areas: Veterinary Sciences |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-10-18 2019-10-18T00:00:00Z 2021-10-27T22:33:33Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/22435 |
| url |
http://hdl.handle.net/10400.5/22435 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Santos MF, Alexandre-Pires G, Pereira MA, Marques CS, Gomes J, Correia J, Duarte A, Gomes L, Rodrigues AV, Basso A, et al. 2019. Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment. Frontiers in Veterinary Sciences. 6:362 10.3389/fvets.2019.00362 Frontiers in Veterinary Sciences |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Dirk Werling, Royal Veterinary College (RVC), United Kingdom |
| publisher.none.fl_str_mv |
Dirk Werling, Royal Veterinary College (RVC), United Kingdom |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799131161273827328 |