Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/4542 |
Resumo: | Infection due to Schistosoma haematobium is carcinogenic. However, the cellular and molecular mechanisms underlying urogenital schistosomiasis (UGS)-induced carcinogenesis have not been well defined. Conceptually, early molecular detection of this phenomenon, through non-invasive procedures, seems feasible and is desirable. Previous analysis of urine collected during UGS suggests that estrogen metabolites, including depurinating adducts, may be useful for this purpose. Here, a new direction was pursued: the identification of molecular pathways and potential biomarkers in S. haematobium-induced bladder cancer by analyzing the proteome profiling of urine samples from UGS patients. GeLC-MS/MS followed by protein-protein interaction analysis indicated oxidative stress and immune defense systems responsible for microbicide activity are the most representative clusters in UGS patients. Proteins involved in immunity, negative regulation of endopeptidase activity, and inflammation were more prevalent in UGS patients with bladder cancer, whereas proteins with roles in renal system process, sensory perception, and gas and oxygen transport were more abundant in subjects with urothelial carcinoma not associated with UGS. These findings highlighted a Th2-type immune response induced by S. haematobium, which seems to be further modulated by tumorigenesis, resulting in high-grade bladder cancer characterized by an inflammatory response and complement activation alternative pathway. These findings established a starting point for the development of multimarker strategies for the early detection of UGS-induced bladder cancer. |
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Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomicsAdolescentAdultAgedAnimalsCarcinoma, Transitional CellCell Transformation, NeoplasticElectrophoresis, Polyacrylamide GelFemaleHumansMaleMiddle AgedProteomicsSchistosoma haematobiumSchistosomiasis haematobiaSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationUrinary Bladder NeoplasmsYoung AdultInfecções Sistémicas e ZoonosesInfection due to Schistosoma haematobium is carcinogenic. However, the cellular and molecular mechanisms underlying urogenital schistosomiasis (UGS)-induced carcinogenesis have not been well defined. Conceptually, early molecular detection of this phenomenon, through non-invasive procedures, seems feasible and is desirable. Previous analysis of urine collected during UGS suggests that estrogen metabolites, including depurinating adducts, may be useful for this purpose. Here, a new direction was pursued: the identification of molecular pathways and potential biomarkers in S. haematobium-induced bladder cancer by analyzing the proteome profiling of urine samples from UGS patients. GeLC-MS/MS followed by protein-protein interaction analysis indicated oxidative stress and immune defense systems responsible for microbicide activity are the most representative clusters in UGS patients. Proteins involved in immunity, negative regulation of endopeptidase activity, and inflammation were more prevalent in UGS patients with bladder cancer, whereas proteins with roles in renal system process, sensory perception, and gas and oxygen transport were more abundant in subjects with urothelial carcinoma not associated with UGS. These findings highlighted a Th2-type immune response induced by S. haematobium, which seems to be further modulated by tumorigenesis, resulting in high-grade bladder cancer characterized by an inflammatory response and complement activation alternative pathway. These findings established a starting point for the development of multimarker strategies for the early detection of UGS-induced bladder cancer.This work was supported by the Portuguese Foundation for Science and Technology (FCT), European Union, QREN, FEDER, and COMPETE for funding the QOPNA; by iBiMED research unit (project PEst-C/QUI/UI0062/2013, UID/BIM/04501/2013, UID/IC/00051/2013, and COST action BM1305) and PhD fellowship SFRH/BD/80855/2011 (CB); and by the Portuguese Mass Spectrometry Network (RNEM).Springer Verlag / International Society of Oncology and BioMarkers (ISOBM)Repositório Científico do Instituto Nacional de SaúdeBernardo, CarinaCunha, Maria CláudiaSantos, Júlio Henriqueda Costa, José M CorreiaBrindley, Paul JLopes, CarlosAmado, FranciscoFerreira, RitaVitorino, RuiSantos, Lúcio Lara2021-09-01T00:30:09Z2016-082016-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4542engTumour Biol. 2016 Aug;37(8):11279-87. doi: 10.1007/s13277-016-4997-y. Epub 2016 Mar 710.1007/s13277-016-4997-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:23Zoai:repositorio.insa.pt:10400.18/4542Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:19.361114Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics |
title |
Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics |
spellingShingle |
Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics Bernardo, Carina Adolescent Adult Aged Animals Carcinoma, Transitional Cell Cell Transformation, Neoplastic Electrophoresis, Polyacrylamide Gel Female Humans Male Middle Aged Proteomics Schistosoma haematobium Schistosomiasis haematobia Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Urinary Bladder Neoplasms Young Adult Infecções Sistémicas e Zoonoses |
title_short |
Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics |
title_full |
Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics |
title_fullStr |
Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics |
title_full_unstemmed |
Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics |
title_sort |
Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics |
author |
Bernardo, Carina |
author_facet |
Bernardo, Carina Cunha, Maria Cláudia Santos, Júlio Henrique da Costa, José M Correia Brindley, Paul J Lopes, Carlos Amado, Francisco Ferreira, Rita Vitorino, Rui Santos, Lúcio Lara |
author_role |
author |
author2 |
Cunha, Maria Cláudia Santos, Júlio Henrique da Costa, José M Correia Brindley, Paul J Lopes, Carlos Amado, Francisco Ferreira, Rita Vitorino, Rui Santos, Lúcio Lara |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Bernardo, Carina Cunha, Maria Cláudia Santos, Júlio Henrique da Costa, José M Correia Brindley, Paul J Lopes, Carlos Amado, Francisco Ferreira, Rita Vitorino, Rui Santos, Lúcio Lara |
dc.subject.por.fl_str_mv |
Adolescent Adult Aged Animals Carcinoma, Transitional Cell Cell Transformation, Neoplastic Electrophoresis, Polyacrylamide Gel Female Humans Male Middle Aged Proteomics Schistosoma haematobium Schistosomiasis haematobia Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Urinary Bladder Neoplasms Young Adult Infecções Sistémicas e Zoonoses |
topic |
Adolescent Adult Aged Animals Carcinoma, Transitional Cell Cell Transformation, Neoplastic Electrophoresis, Polyacrylamide Gel Female Humans Male Middle Aged Proteomics Schistosoma haematobium Schistosomiasis haematobia Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Urinary Bladder Neoplasms Young Adult Infecções Sistémicas e Zoonoses |
description |
Infection due to Schistosoma haematobium is carcinogenic. However, the cellular and molecular mechanisms underlying urogenital schistosomiasis (UGS)-induced carcinogenesis have not been well defined. Conceptually, early molecular detection of this phenomenon, through non-invasive procedures, seems feasible and is desirable. Previous analysis of urine collected during UGS suggests that estrogen metabolites, including depurinating adducts, may be useful for this purpose. Here, a new direction was pursued: the identification of molecular pathways and potential biomarkers in S. haematobium-induced bladder cancer by analyzing the proteome profiling of urine samples from UGS patients. GeLC-MS/MS followed by protein-protein interaction analysis indicated oxidative stress and immune defense systems responsible for microbicide activity are the most representative clusters in UGS patients. Proteins involved in immunity, negative regulation of endopeptidase activity, and inflammation were more prevalent in UGS patients with bladder cancer, whereas proteins with roles in renal system process, sensory perception, and gas and oxygen transport were more abundant in subjects with urothelial carcinoma not associated with UGS. These findings highlighted a Th2-type immune response induced by S. haematobium, which seems to be further modulated by tumorigenesis, resulting in high-grade bladder cancer characterized by an inflammatory response and complement activation alternative pathway. These findings established a starting point for the development of multimarker strategies for the early detection of UGS-induced bladder cancer. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08 2016-08-01T00:00:00Z 2021-09-01T00:30:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/4542 |
url |
http://hdl.handle.net/10400.18/4542 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tumour Biol. 2016 Aug;37(8):11279-87. doi: 10.1007/s13277-016-4997-y. Epub 2016 Mar 7 10.1007/s13277-016-4997-y |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag / International Society of Oncology and BioMarkers (ISOBM) |
publisher.none.fl_str_mv |
Springer Verlag / International Society of Oncology and BioMarkers (ISOBM) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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