Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation

Detalhes bibliográficos
Autor(a) principal: Pereira, H. Sofia
Data de Publicação: 2009
Outros Autores: Barão, Augusta, Caperta, A., Rocha, João, Viegas, Wanda, Delgado, Margarida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/2851
Resumo: Research Article
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spelling Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregationE3900 rye B repeatdevelopmentally regulated transcriptionchromosome segregationResearch ArticleTwo sequence families, E3900 and D1100, are amplified on the subtelomeric domain of the long arm of rye B chromosomes, the region that controls its drive mechanism. In this work, polymerase chain reaction (PCR) with a number of primers spanning E3900 shows that the organization and nucleotide sequence of E3900-related portions are present and highly conserved on rye A chromosomes as well as in other cereals. Quantitative Real-Time PCR estimates two E3900 sequences to be represented in 100–150 copies on Bs and at least as single copies on As. A novel E3900- related sequence, with a deletion that results in a frameshift and subsequently an open reading frame with putative DNA binding motifs, is identified. Expression analysis of E3900 indicates identical transcription levels in leaves from plants with and without Bs, showing that the expression of these sequences must be silenced on Bs and tightly regulated on As in leaves. In contrast, E3900 transcription is upregulated during meiosis exclusively in plants with Bs, maintaining a high level of transcription in the gametophyte. Interestingly, Bs not only influence their own chromatid segregation but also that of the regular chromosome complement of both rye and wheat. There is a drastic increase in frequency of disrupted metaphase and anaphase cells in the first mitosis of pollen grains carrying Bs, which appears to be due to anomalous adherences between sister chromatids. Taken together, this work provides insight into how E3900 sequences are potentially associated with important evolutionary mechanisms involved in basic cellular processes.Oxford University PressRepositório da Universidade de LisboaPereira, H. SofiaBarão, AugustaCaperta, A.Rocha, JoãoViegas, WandaDelgado, Margarida2011-01-24T16:01:40Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/2851eng"Molecular Biology and Evolution". 26:8 (2009) 1683-1697DOI: 10.1093/molbev/msp082info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:34:00Zoai:www.repository.utl.pt:10400.5/2851Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:50:49.603501Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
title Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
spellingShingle Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
Pereira, H. Sofia
E3900 rye B repeat
developmentally regulated transcription
chromosome segregation
title_short Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
title_full Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
title_fullStr Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
title_full_unstemmed Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
title_sort Rye Bs disclose ancestral sequences in cereal genomes with a potential role in gametophyte chromatid segregation
author Pereira, H. Sofia
author_facet Pereira, H. Sofia
Barão, Augusta
Caperta, A.
Rocha, João
Viegas, Wanda
Delgado, Margarida
author_role author
author2 Barão, Augusta
Caperta, A.
Rocha, João
Viegas, Wanda
Delgado, Margarida
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pereira, H. Sofia
Barão, Augusta
Caperta, A.
Rocha, João
Viegas, Wanda
Delgado, Margarida
dc.subject.por.fl_str_mv E3900 rye B repeat
developmentally regulated transcription
chromosome segregation
topic E3900 rye B repeat
developmentally regulated transcription
chromosome segregation
description Research Article
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2011-01-24T16:01:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/2851
url http://hdl.handle.net/10400.5/2851
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Molecular Biology and Evolution". 26:8 (2009) 1683-1697
DOI: 10.1093/molbev/msp082
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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