Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing

Detalhes bibliográficos
Autor(a) principal: Nojima, Takayuki
Data de Publicação: 2015
Outros Autores: Gomes, Tomás, Grosso, Ana Rita, Kimura, Hiroshi, Dye, Michael J., Dhir, Somdutta, Carmo-Fonseca, Maria, Proudfoot, Nicholas J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51070
Resumo: © Copyright © 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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spelling Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing© Copyright © 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)Transcription is a highly dynamic process. Consequently, we have developed native elongating transcript sequencing technology for mammalian chromatin (mNET-seq), which generates single-nucleotide resolution, nascent transcription profiles. Nascent RNA was detected in the active site of RNA polymerase II (Pol II) along with associated RNA processing intermediates. In particular, we detected 5'splice site cleavage by the spliceosome, showing that cleaved upstream exon transcripts are associated with Pol II CTD phosphorylated on the serine 5 position (S5P), which is accumulated over downstream exons. Also, depletion of termination factors substantially reduces Pol II pausing at gene ends, leading to termination defects. Notably, termination factors play an additional promoter role by restricting non-productive RNA synthesis in a Pol II CTD S2P-specific manner. Our results suggest that CTD phosphorylation patterns established for yeast transcription are significantly different in mammals. Taken together, mNET-seq provides dynamic and detailed snapshots of the complex events underlying transcription in mammals.This work was supported by funding to N.J.P. (Wellcome Trust Programme [091805/Z/10/Z] and ERC Advanced [339270] Grants) and to M.C.-F. (Fundação Ciência e Tecnologia, Portugal).ElsevierRepositório da Universidade de LisboaNojima, TakayukiGomes, TomásGrosso, Ana RitaKimura, HiroshiDye, Michael J.Dhir, SomduttaCarmo-Fonseca, MariaProudfoot, Nicholas J.2022-02-01T15:19:38Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51070engCell 161, 526–540, April 23, 20150092-867410.1016/j.cell.2015.03.0271097-4172info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:55:32Zoai:repositorio.ul.pt:10451/51070Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:23.564617Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing
title Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing
spellingShingle Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing
Nojima, Takayuki
title_short Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing
title_full Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing
title_fullStr Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing
title_full_unstemmed Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing
title_sort Mammalian NET-Seq reveals genome-wide nascent transcription coupled to RNA processing
author Nojima, Takayuki
author_facet Nojima, Takayuki
Gomes, Tomás
Grosso, Ana Rita
Kimura, Hiroshi
Dye, Michael J.
Dhir, Somdutta
Carmo-Fonseca, Maria
Proudfoot, Nicholas J.
author_role author
author2 Gomes, Tomás
Grosso, Ana Rita
Kimura, Hiroshi
Dye, Michael J.
Dhir, Somdutta
Carmo-Fonseca, Maria
Proudfoot, Nicholas J.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Nojima, Takayuki
Gomes, Tomás
Grosso, Ana Rita
Kimura, Hiroshi
Dye, Michael J.
Dhir, Somdutta
Carmo-Fonseca, Maria
Proudfoot, Nicholas J.
description © Copyright © 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2022-02-01T15:19:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51070
url http://hdl.handle.net/10451/51070
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cell 161, 526–540, April 23, 2015
0092-8674
10.1016/j.cell.2015.03.027
1097-4172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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