Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data

Detalhes bibliográficos
Autor(a) principal: Prudêncio, Pedro
Data de Publicação: 2020
Outros Autores: Rebelo, Kenny, Grosso, Ana Rita, Martinho, Rui Gonçalo, Carmo-Fonseca, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/108217
Resumo: Fundação para a Ciência e Tecnologia (FCT)/ Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado (UID/BIM/50005/2019), and FCT/FEDER/POR Lisboa 2020, Programa Operacional Regional de Lisboa, PORTUGAL 2020 (LISBOA-01-0145-FEDER-016394).. R.G.M. is supported by FCT grants PTDC/BEX-BID/0395/2014 , PTDC/BIA-BID/28441/2017
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network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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spelling Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput dataCo-transcriptional splicingmNET-seqNascent RNARNA polymerase IIMolecular BiologyBiochemistry, Genetics and Molecular Biology(all)Fundação para a Ciência e Tecnologia (FCT)/ Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado (UID/BIM/50005/2019), and FCT/FEDER/POR Lisboa 2020, Programa Operacional Regional de Lisboa, PORTUGAL 2020 (LISBOA-01-0145-FEDER-016394).. R.G.M. is supported by FCT grants PTDC/BEX-BID/0395/2014 , PTDC/BIA-BID/28441/2017Mammalian Native Elongating Transcript sequencing (mNET-seq) is a recently developed technique that generates genome-wide profiles of nascent transcripts associated with RNA polymerase II (Pol II) elongation complexes. The ternary transcription complexes formed by Pol II, DNA template and nascent RNA are first isolated, without crosslinking, by immunoprecipitation with antibodies that specifically recognize the different phosphorylation states of the polymerase large subunit C-terminal domain (CTD). The coordinate of the 3′ end of the RNA in the complexes is then identified by high-throughput sequencing. The main advantage of mNET-seq is that it provides global, bidirectional maps of Pol II CTD phosphorylation-specific nascent transcripts and coupled RNA processing at single nucleotide resolution. Here we describe the general pipeline to prepare and analyse high-throughput data from mNET-seq experiments.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNPrudêncio, PedroRebelo, KennyGrosso, Ana RitaMartinho, Rui GonçaloCarmo-Fonseca, Maria2020-12-05T00:22:21Z2020-06-012020-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/108217eng1046-2023PURE: 14820228https://doi.org/10.1016/j.ymeth.2019.09.003info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:49:08Zoai:run.unl.pt:10362/108217Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:49:08Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data
title Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data
spellingShingle Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data
Prudêncio, Pedro
Co-transcriptional splicing
mNET-seq
Nascent RNA
RNA polymerase II
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
title_short Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data
title_full Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data
title_fullStr Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data
title_full_unstemmed Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data
title_sort Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-throughput data
author Prudêncio, Pedro
author_facet Prudêncio, Pedro
Rebelo, Kenny
Grosso, Ana Rita
Martinho, Rui Gonçalo
Carmo-Fonseca, Maria
author_role author
author2 Rebelo, Kenny
Grosso, Ana Rita
Martinho, Rui Gonçalo
Carmo-Fonseca, Maria
author2_role author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Prudêncio, Pedro
Rebelo, Kenny
Grosso, Ana Rita
Martinho, Rui Gonçalo
Carmo-Fonseca, Maria
dc.subject.por.fl_str_mv Co-transcriptional splicing
mNET-seq
Nascent RNA
RNA polymerase II
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
topic Co-transcriptional splicing
mNET-seq
Nascent RNA
RNA polymerase II
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
description Fundação para a Ciência e Tecnologia (FCT)/ Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado (UID/BIM/50005/2019), and FCT/FEDER/POR Lisboa 2020, Programa Operacional Regional de Lisboa, PORTUGAL 2020 (LISBOA-01-0145-FEDER-016394).. R.G.M. is supported by FCT grants PTDC/BEX-BID/0395/2014 , PTDC/BIA-BID/28441/2017
publishDate 2020
dc.date.none.fl_str_mv 2020-12-05T00:22:21Z
2020-06-01
2020-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/108217
url http://hdl.handle.net/10362/108217
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1046-2023
PURE: 14820228
https://doi.org/10.1016/j.ymeth.2019.09.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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