Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/11481 |
Resumo: | DNA has been described as a structural component of the extracellular matrix (ECM) in bacterial biofilms. In Candida albicans, there is a scarce knowledge concerning the contribution of extracellular DNA (eDNA) to biofilm matrix and overall structure. This work examined the presence and quantified the amount of eDNA in C. albicans biofilm ECM and the effect of DNase treatment and the addition of exogenous DNA on C. albicans biofilm development as indicators of a role for eDNA in biofilm development. We were able to detect the accumulation of eDNA in biofilm ECM extracted from C. albicans biofilms formed under conditions of flow, although the quantity of eDNA detected differed according to growth conditions, in particular with regards to the medium used to grow the biofilms. Experiments with C. albicans biofilms formed statically using a microtiter plate model indicated that the addition of exogenous DNA (>160 ng/ml) increases biofilm biomass and, conversely, DNase treatment (>0.03 mg/ml) decreases biofilm biomass at later time points of biofilm development. We present evidence for the role of eDNA in C. albicans biofilm structure and formation, consistent with eDNA being a key element of the ECM in mature C. albicans biofilms and playing a predominant role in biofilm structural integrity and maintenance. |
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Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilmsC. albicansBiofilmExtracellular matrixExtracellular DNADNaseScience & TechnologyDNA has been described as a structural component of the extracellular matrix (ECM) in bacterial biofilms. In Candida albicans, there is a scarce knowledge concerning the contribution of extracellular DNA (eDNA) to biofilm matrix and overall structure. This work examined the presence and quantified the amount of eDNA in C. albicans biofilm ECM and the effect of DNase treatment and the addition of exogenous DNA on C. albicans biofilm development as indicators of a role for eDNA in biofilm development. We were able to detect the accumulation of eDNA in biofilm ECM extracted from C. albicans biofilms formed under conditions of flow, although the quantity of eDNA detected differed according to growth conditions, in particular with regards to the medium used to grow the biofilms. Experiments with C. albicans biofilms formed statically using a microtiter plate model indicated that the addition of exogenous DNA (>160 ng/ml) increases biofilm biomass and, conversely, DNase treatment (>0.03 mg/ml) decreases biofilm biomass at later time points of biofilm development. We present evidence for the role of eDNA in C. albicans biofilm structure and formation, consistent with eDNA being a key element of the ECM in mature C. albicans biofilms and playing a predominant role in biofilm structural integrity and maintenance.National Institute of Dental & Craniofacial ResearchFundação para a Ciência e Tecnologia (FCT) - SFRH/BD/28222/2006National Institute of Allergy and Infectious DiseasesSpringerUniversidade do MinhoMartins, Margarida Isabel Barros CoelhoUppuluri, PriyaThomas, Derek P.Cleary, Ian A.Henriques, MarianaLopez-Ribot, José L.Oliveira, Rosário20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/11481eng"Mycopathologia". ISSN 0301-486X. 169:5 (2010) 323-331.0301-486X10.1007/s11046-009-9264-y20012895info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:27Zoai:repositorium.sdum.uminho.pt:1822/11481Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:40:56.563972Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms |
title |
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms |
spellingShingle |
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms Martins, Margarida Isabel Barros Coelho C. albicans Biofilm Extracellular matrix Extracellular DNA DNase Science & Technology |
title_short |
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms |
title_full |
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms |
title_fullStr |
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms |
title_full_unstemmed |
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms |
title_sort |
Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms |
author |
Martins, Margarida Isabel Barros Coelho |
author_facet |
Martins, Margarida Isabel Barros Coelho Uppuluri, Priya Thomas, Derek P. Cleary, Ian A. Henriques, Mariana Lopez-Ribot, José L. Oliveira, Rosário |
author_role |
author |
author2 |
Uppuluri, Priya Thomas, Derek P. Cleary, Ian A. Henriques, Mariana Lopez-Ribot, José L. Oliveira, Rosário |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Margarida Isabel Barros Coelho Uppuluri, Priya Thomas, Derek P. Cleary, Ian A. Henriques, Mariana Lopez-Ribot, José L. Oliveira, Rosário |
dc.subject.por.fl_str_mv |
C. albicans Biofilm Extracellular matrix Extracellular DNA DNase Science & Technology |
topic |
C. albicans Biofilm Extracellular matrix Extracellular DNA DNase Science & Technology |
description |
DNA has been described as a structural component of the extracellular matrix (ECM) in bacterial biofilms. In Candida albicans, there is a scarce knowledge concerning the contribution of extracellular DNA (eDNA) to biofilm matrix and overall structure. This work examined the presence and quantified the amount of eDNA in C. albicans biofilm ECM and the effect of DNase treatment and the addition of exogenous DNA on C. albicans biofilm development as indicators of a role for eDNA in biofilm development. We were able to detect the accumulation of eDNA in biofilm ECM extracted from C. albicans biofilms formed under conditions of flow, although the quantity of eDNA detected differed according to growth conditions, in particular with regards to the medium used to grow the biofilms. Experiments with C. albicans biofilms formed statically using a microtiter plate model indicated that the addition of exogenous DNA (>160 ng/ml) increases biofilm biomass and, conversely, DNase treatment (>0.03 mg/ml) decreases biofilm biomass at later time points of biofilm development. We present evidence for the role of eDNA in C. albicans biofilm structure and formation, consistent with eDNA being a key element of the ECM in mature C. albicans biofilms and playing a predominant role in biofilm structural integrity and maintenance. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/11481 |
url |
http://hdl.handle.net/1822/11481 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Mycopathologia". ISSN 0301-486X. 169:5 (2010) 323-331. 0301-486X 10.1007/s11046-009-9264-y 20012895 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132956560719872 |