Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/20002297.2017.1385372 http://hdl.handle.net/11449/163552 |
Resumo: | Background: Infections caused by Candida spp. have been associated with formation of a biofilm, i.e. a complex microstructure of cells adhering to a surface and embedded within an extracellular matrix (ECM). Methods: The ECMs of a wild-type (WT, SN425) and two Candida albicans mutant strains, Delta/Delta tec1 (CJN2330) and Delta/Delta efg1 (CJN2302), were evaluated. Colony-forming units (cfu), total biomass (mg), water-soluble polysaccharides (WSPs), alkali-soluble polysaccharides (ASPs), proteins (insoluble part of biofilms and matrix proteins), and extracellular DNA (eDNA) were quantified. Variable-pressure scanning electron microscopy and confocal scanning laser microscopy were performed. The biovolume (mu m(3)/mu m(2)) and maximum thickness (mu m) of the biofilms were quantified using COMSTAT2. Results: ASP content was highest in WT (mean +/- SD: 74.5 +/- 22.0 mu g), followed by Delta/Delta tec1 (44.0 +/- 24.1 mu g) and Delta/Delta efg1 (14.7 +/- 5.0 mu g). The protein correlated with ASPs (r = 0.666) and with matrix proteins (r = 0.670) in the WT strain. The population in Delta/Delta efg1 correlated with the protein (r = 0.734) and its biofilms exhibited the lowest biomass and biovolume, and maximum thickness. In Delta/Delta tec1, ASP correlated with eDNA (r = 0.678). Conclusion: ASP production may be linked to C. albicans cell filamentous morphology. |
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Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphologyCandida albicansbiofilmEFG1TEC1extracellular matrixBackground: Infections caused by Candida spp. have been associated with formation of a biofilm, i.e. a complex microstructure of cells adhering to a surface and embedded within an extracellular matrix (ECM). Methods: The ECMs of a wild-type (WT, SN425) and two Candida albicans mutant strains, Delta/Delta tec1 (CJN2330) and Delta/Delta efg1 (CJN2302), were evaluated. Colony-forming units (cfu), total biomass (mg), water-soluble polysaccharides (WSPs), alkali-soluble polysaccharides (ASPs), proteins (insoluble part of biofilms and matrix proteins), and extracellular DNA (eDNA) were quantified. Variable-pressure scanning electron microscopy and confocal scanning laser microscopy were performed. The biovolume (mu m(3)/mu m(2)) and maximum thickness (mu m) of the biofilms were quantified using COMSTAT2. Results: ASP content was highest in WT (mean +/- SD: 74.5 +/- 22.0 mu g), followed by Delta/Delta tec1 (44.0 +/- 24.1 mu g) and Delta/Delta efg1 (14.7 +/- 5.0 mu g). The protein correlated with ASPs (r = 0.666) and with matrix proteins (r = 0.670) in the WT strain. The population in Delta/Delta efg1 correlated with the protein (r = 0.734) and its biofilms exhibited the lowest biomass and biovolume, and maximum thickness. In Delta/Delta tec1, ASP correlated with eDNA (r = 0.678). Conclusion: ASP production may be linked to C. albicans cell filamentous morphology.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, BrazilIndiana Univ, Sch Dent, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN 46202 USASao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, BrazilFAPESP: 2014/18804-1FAPESP: 2016/00256-3FAPESP: 2014/50857-8CNPq: 465360/2014-9Taylor & Francis LtdUniversidade Estadual Paulista (Unesp)Indiana UnivDias Panariello, Beatriz Helena [UNESP]Klein, Marlise I. [UNESP]Pavarina, Ana Claudia [UNESP]Duarte, Simone2018-11-26T17:42:30Z2018-11-26T17:42:30Z2017-10-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1080/20002297.2017.1385372Journal Of Oral Microbiology. Abingdon: Taylor & Francis Ltd, v. 9, 11 p., 2017.2000-2297http://hdl.handle.net/11449/16355210.1080/20002297.2017.1385372WOS:000416980700001WOS000416980700001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Oral Microbiology1,541info:eu-repo/semantics/openAccess2023-10-20T06:04:04Zoai:repositorio.unesp.br:11449/163552Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-20T06:04:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology |
title |
Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology |
spellingShingle |
Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology Dias Panariello, Beatriz Helena [UNESP] Candida albicans biofilm EFG1 TEC1 extracellular matrix |
title_short |
Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology |
title_full |
Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology |
title_fullStr |
Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology |
title_full_unstemmed |
Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology |
title_sort |
Inactivation of genes TEC1 and EFG1 in Candida albicans influences extracellular matrix composition and biofilm morphology |
author |
Dias Panariello, Beatriz Helena [UNESP] |
author_facet |
Dias Panariello, Beatriz Helena [UNESP] Klein, Marlise I. [UNESP] Pavarina, Ana Claudia [UNESP] Duarte, Simone |
author_role |
author |
author2 |
Klein, Marlise I. [UNESP] Pavarina, Ana Claudia [UNESP] Duarte, Simone |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Indiana Univ |
dc.contributor.author.fl_str_mv |
Dias Panariello, Beatriz Helena [UNESP] Klein, Marlise I. [UNESP] Pavarina, Ana Claudia [UNESP] Duarte, Simone |
dc.subject.por.fl_str_mv |
Candida albicans biofilm EFG1 TEC1 extracellular matrix |
topic |
Candida albicans biofilm EFG1 TEC1 extracellular matrix |
description |
Background: Infections caused by Candida spp. have been associated with formation of a biofilm, i.e. a complex microstructure of cells adhering to a surface and embedded within an extracellular matrix (ECM). Methods: The ECMs of a wild-type (WT, SN425) and two Candida albicans mutant strains, Delta/Delta tec1 (CJN2330) and Delta/Delta efg1 (CJN2302), were evaluated. Colony-forming units (cfu), total biomass (mg), water-soluble polysaccharides (WSPs), alkali-soluble polysaccharides (ASPs), proteins (insoluble part of biofilms and matrix proteins), and extracellular DNA (eDNA) were quantified. Variable-pressure scanning electron microscopy and confocal scanning laser microscopy were performed. The biovolume (mu m(3)/mu m(2)) and maximum thickness (mu m) of the biofilms were quantified using COMSTAT2. Results: ASP content was highest in WT (mean +/- SD: 74.5 +/- 22.0 mu g), followed by Delta/Delta tec1 (44.0 +/- 24.1 mu g) and Delta/Delta efg1 (14.7 +/- 5.0 mu g). The protein correlated with ASPs (r = 0.666) and with matrix proteins (r = 0.670) in the WT strain. The population in Delta/Delta efg1 correlated with the protein (r = 0.734) and its biofilms exhibited the lowest biomass and biovolume, and maximum thickness. In Delta/Delta tec1, ASP correlated with eDNA (r = 0.678). Conclusion: ASP production may be linked to C. albicans cell filamentous morphology. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-17 2018-11-26T17:42:30Z 2018-11-26T17:42:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/20002297.2017.1385372 Journal Of Oral Microbiology. Abingdon: Taylor & Francis Ltd, v. 9, 11 p., 2017. 2000-2297 http://hdl.handle.net/11449/163552 10.1080/20002297.2017.1385372 WOS:000416980700001 WOS000416980700001.pdf |
url |
http://dx.doi.org/10.1080/20002297.2017.1385372 http://hdl.handle.net/11449/163552 |
identifier_str_mv |
Journal Of Oral Microbiology. Abingdon: Taylor & Francis Ltd, v. 9, 11 p., 2017. 2000-2297 10.1080/20002297.2017.1385372 WOS:000416980700001 WOS000416980700001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Oral Microbiology 1,541 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis Ltd |
publisher.none.fl_str_mv |
Taylor & Francis Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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