Discovery of the first pectin methylesterase gene in a plant-parasitic nematode

Detalhes bibliográficos
Autor(a) principal: Vicente, Claudia
Data de Publicação: 2019
Outros Autores: Nemchinov, Lev, Mota, Manuel, Kamo, K, Vieira, Paulo
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/29023
https://doi.org/10.1094/MPMI-32- 10-S1.1
Resumo: The root lesion nematode (RLN) Pratylenchus penetrans is considered one of the most damaging plant-parasitic nematode (PPN) species worldwide affecting important agronomic and horticultural crops (e.g. potato, corn). The successful invasion of roots by RLNs is related to their ability to overcome the barrier imposed by the plant cell wall. Like other PPNs, RLNs are equipped with a protrusible stylet that mechanically disrupts the cell wall and through which cell wall-degrading enzymes are secreted to facilitate penetration and migration of the nematode through host roots. Herein, we identified and characterized a pectin methylesterase gene for P. penetrans. Sequence analysis confirm the eukaryotic gene structure of Pp-pme. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato Virus X-based vector. PMEs have been so far described for plants, fungus, bacteria, and to a restrict number of insects. To our knowledge, this is the first report a PME within the phylum Nematoda.
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spelling Discovery of the first pectin methylesterase gene in a plant-parasitic nematodePectin EsteraseThe root lesion nematode (RLN) Pratylenchus penetrans is considered one of the most damaging plant-parasitic nematode (PPN) species worldwide affecting important agronomic and horticultural crops (e.g. potato, corn). The successful invasion of roots by RLNs is related to their ability to overcome the barrier imposed by the plant cell wall. Like other PPNs, RLNs are equipped with a protrusible stylet that mechanically disrupts the cell wall and through which cell wall-degrading enzymes are secreted to facilitate penetration and migration of the nematode through host roots. Herein, we identified and characterized a pectin methylesterase gene for P. penetrans. Sequence analysis confirm the eukaryotic gene structure of Pp-pme. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato Virus X-based vector. PMEs have been so far described for plants, fungus, bacteria, and to a restrict number of insects. To our knowledge, this is the first report a PME within the phylum Nematoda.Molecular Plant-Microbe Interactions2021-01-29T18:29:44Z2021-01-292019-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/29023http://hdl.handle.net/10174/29023https://doi.org/10.1094/MPMI-32- 10-S1.1engnaonaosimcvicente@uevora.ptndmmota@uevora.ptndndVicente, ClaudiaNemchinov, LevMota, ManuelKamo, KVieira, Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:25:39Zoai:dspace.uevora.pt:10174/29023Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:18:44.012743Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
title Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
spellingShingle Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
Vicente, Claudia
Pectin Esterase
title_short Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
title_full Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
title_fullStr Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
title_full_unstemmed Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
title_sort Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
author Vicente, Claudia
author_facet Vicente, Claudia
Nemchinov, Lev
Mota, Manuel
Kamo, K
Vieira, Paulo
author_role author
author2 Nemchinov, Lev
Mota, Manuel
Kamo, K
Vieira, Paulo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vicente, Claudia
Nemchinov, Lev
Mota, Manuel
Kamo, K
Vieira, Paulo
dc.subject.por.fl_str_mv Pectin Esterase
topic Pectin Esterase
description The root lesion nematode (RLN) Pratylenchus penetrans is considered one of the most damaging plant-parasitic nematode (PPN) species worldwide affecting important agronomic and horticultural crops (e.g. potato, corn). The successful invasion of roots by RLNs is related to their ability to overcome the barrier imposed by the plant cell wall. Like other PPNs, RLNs are equipped with a protrusible stylet that mechanically disrupts the cell wall and through which cell wall-degrading enzymes are secreted to facilitate penetration and migration of the nematode through host roots. Herein, we identified and characterized a pectin methylesterase gene for P. penetrans. Sequence analysis confirm the eukaryotic gene structure of Pp-pme. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato Virus X-based vector. PMEs have been so far described for plants, fungus, bacteria, and to a restrict number of insects. To our knowledge, this is the first report a PME within the phylum Nematoda.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-01T00:00:00Z
2021-01-29T18:29:44Z
2021-01-29
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/29023
http://hdl.handle.net/10174/29023
https://doi.org/10.1094/MPMI-32- 10-S1.1
url http://hdl.handle.net/10174/29023
https://doi.org/10.1094/MPMI-32- 10-S1.1
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cvicente@uevora.pt
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mmota@uevora.pt
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dc.publisher.none.fl_str_mv Molecular Plant-Microbe Interactions
publisher.none.fl_str_mv Molecular Plant-Microbe Interactions
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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