Discovery of the first pectin methylesterase gene in a plant-parasitic nematode
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/29023 https://doi.org/10.1094/MPMI-32- 10-S1.1 |
Resumo: | The root lesion nematode (RLN) Pratylenchus penetrans is considered one of the most damaging plant-parasitic nematode (PPN) species worldwide affecting important agronomic and horticultural crops (e.g. potato, corn). The successful invasion of roots by RLNs is related to their ability to overcome the barrier imposed by the plant cell wall. Like other PPNs, RLNs are equipped with a protrusible stylet that mechanically disrupts the cell wall and through which cell wall-degrading enzymes are secreted to facilitate penetration and migration of the nematode through host roots. Herein, we identified and characterized a pectin methylesterase gene for P. penetrans. Sequence analysis confirm the eukaryotic gene structure of Pp-pme. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato Virus X-based vector. PMEs have been so far described for plants, fungus, bacteria, and to a restrict number of insects. To our knowledge, this is the first report a PME within the phylum Nematoda. |
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Discovery of the first pectin methylesterase gene in a plant-parasitic nematodePectin EsteraseThe root lesion nematode (RLN) Pratylenchus penetrans is considered one of the most damaging plant-parasitic nematode (PPN) species worldwide affecting important agronomic and horticultural crops (e.g. potato, corn). The successful invasion of roots by RLNs is related to their ability to overcome the barrier imposed by the plant cell wall. Like other PPNs, RLNs are equipped with a protrusible stylet that mechanically disrupts the cell wall and through which cell wall-degrading enzymes are secreted to facilitate penetration and migration of the nematode through host roots. Herein, we identified and characterized a pectin methylesterase gene for P. penetrans. Sequence analysis confirm the eukaryotic gene structure of Pp-pme. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato Virus X-based vector. PMEs have been so far described for plants, fungus, bacteria, and to a restrict number of insects. To our knowledge, this is the first report a PME within the phylum Nematoda.Molecular Plant-Microbe Interactions2021-01-29T18:29:44Z2021-01-292019-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/29023http://hdl.handle.net/10174/29023https://doi.org/10.1094/MPMI-32- 10-S1.1engnaonaosimcvicente@uevora.ptndmmota@uevora.ptndndVicente, ClaudiaNemchinov, LevMota, ManuelKamo, KVieira, Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:25:39Zoai:dspace.uevora.pt:10174/29023Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:18:44.012743Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Discovery of the first pectin methylesterase gene in a plant-parasitic nematode |
title |
Discovery of the first pectin methylesterase gene in a plant-parasitic nematode |
spellingShingle |
Discovery of the first pectin methylesterase gene in a plant-parasitic nematode Vicente, Claudia Pectin Esterase |
title_short |
Discovery of the first pectin methylesterase gene in a plant-parasitic nematode |
title_full |
Discovery of the first pectin methylesterase gene in a plant-parasitic nematode |
title_fullStr |
Discovery of the first pectin methylesterase gene in a plant-parasitic nematode |
title_full_unstemmed |
Discovery of the first pectin methylesterase gene in a plant-parasitic nematode |
title_sort |
Discovery of the first pectin methylesterase gene in a plant-parasitic nematode |
author |
Vicente, Claudia |
author_facet |
Vicente, Claudia Nemchinov, Lev Mota, Manuel Kamo, K Vieira, Paulo |
author_role |
author |
author2 |
Nemchinov, Lev Mota, Manuel Kamo, K Vieira, Paulo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Vicente, Claudia Nemchinov, Lev Mota, Manuel Kamo, K Vieira, Paulo |
dc.subject.por.fl_str_mv |
Pectin Esterase |
topic |
Pectin Esterase |
description |
The root lesion nematode (RLN) Pratylenchus penetrans is considered one of the most damaging plant-parasitic nematode (PPN) species worldwide affecting important agronomic and horticultural crops (e.g. potato, corn). The successful invasion of roots by RLNs is related to their ability to overcome the barrier imposed by the plant cell wall. Like other PPNs, RLNs are equipped with a protrusible stylet that mechanically disrupts the cell wall and through which cell wall-degrading enzymes are secreted to facilitate penetration and migration of the nematode through host roots. Herein, we identified and characterized a pectin methylesterase gene for P. penetrans. Sequence analysis confirm the eukaryotic gene structure of Pp-pme. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato Virus X-based vector. PMEs have been so far described for plants, fungus, bacteria, and to a restrict number of insects. To our knowledge, this is the first report a PME within the phylum Nematoda. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-01T00:00:00Z 2021-01-29T18:29:44Z 2021-01-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/29023 http://hdl.handle.net/10174/29023 https://doi.org/10.1094/MPMI-32- 10-S1.1 |
url |
http://hdl.handle.net/10174/29023 https://doi.org/10.1094/MPMI-32- 10-S1.1 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
nao nao sim cvicente@uevora.pt nd mmota@uevora.pt nd nd |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Molecular Plant-Microbe Interactions |
publisher.none.fl_str_mv |
Molecular Plant-Microbe Interactions |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136669065019392 |