Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans

Detalhes bibliográficos
Autor(a) principal: Vicente, Cláudia
Data de Publicação: 2019
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/27522
Resumo: Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda.
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spelling Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetransPratylenchus penetransPectin methyl esteraseSimilar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda.PLoS2020-03-02T10:33:47Z2020-03-022019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/27522http://hdl.handle.net/10174/27522engVicente, C.S.L., L.G. Nemchinov, M. Mota, J.D. Eisenback, K. Kamo, and P. Vieira. 2019. Identification and characterization of the first pectin methylesterase gene found in the root lesion nematode Pratylenchus penetrans. PLoS One. 2019 Feb 22;14(2):e0212540. doi: 10.1371/journal.pone.0212540.cvicente@uevora.pt581doi: 10.1371/journal.pone.0212540Vicente, Cláudiainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:22:56Zoai:dspace.uevora.pt:10174/27522Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:32.076841Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
title Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
spellingShingle Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
Vicente, Cláudia
Pratylenchus penetrans
Pectin methyl esterase
title_short Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
title_full Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
title_fullStr Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
title_full_unstemmed Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
title_sort Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
author Vicente, Cláudia
author_facet Vicente, Cláudia
author_role author
dc.contributor.author.fl_str_mv Vicente, Cláudia
dc.subject.por.fl_str_mv Pratylenchus penetrans
Pectin methyl esterase
topic Pratylenchus penetrans
Pectin methyl esterase
description Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01T00:00:00Z
2020-03-02T10:33:47Z
2020-03-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/27522
http://hdl.handle.net/10174/27522
url http://hdl.handle.net/10174/27522
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vicente, C.S.L., L.G. Nemchinov, M. Mota, J.D. Eisenback, K. Kamo, and P. Vieira. 2019. Identification and characterization of the first pectin methylesterase gene found in the root lesion nematode Pratylenchus penetrans. PLoS One. 2019 Feb 22;14(2):e0212540. doi: 10.1371/journal.pone.0212540.
cvicente@uevora.pt
581
doi: 10.1371/journal.pone.0212540
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dc.publisher.none.fl_str_mv PLoS
publisher.none.fl_str_mv PLoS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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