Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/27522 |
Resumo: | Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetransPratylenchus penetransPectin methyl esteraseSimilar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda.PLoS2020-03-02T10:33:47Z2020-03-022019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/27522http://hdl.handle.net/10174/27522engVicente, C.S.L., L.G. Nemchinov, M. Mota, J.D. Eisenback, K. Kamo, and P. Vieira. 2019. Identification and characterization of the first pectin methylesterase gene found in the root lesion nematode Pratylenchus penetrans. PLoS One. 2019 Feb 22;14(2):e0212540. doi: 10.1371/journal.pone.0212540.cvicente@uevora.pt581doi: 10.1371/journal.pone.0212540Vicente, Cláudiainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:22:56Zoai:dspace.uevora.pt:10174/27522Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:32.076841Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans |
title |
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans |
spellingShingle |
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans Vicente, Cláudia Pratylenchus penetrans Pectin methyl esterase |
title_short |
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans |
title_full |
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans |
title_fullStr |
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans |
title_full_unstemmed |
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans |
title_sort |
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans |
author |
Vicente, Cláudia |
author_facet |
Vicente, Cláudia |
author_role |
author |
dc.contributor.author.fl_str_mv |
Vicente, Cláudia |
dc.subject.por.fl_str_mv |
Pratylenchus penetrans Pectin methyl esterase |
topic |
Pratylenchus penetrans Pectin methyl esterase |
description |
Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01T00:00:00Z 2020-03-02T10:33:47Z 2020-03-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/27522 http://hdl.handle.net/10174/27522 |
url |
http://hdl.handle.net/10174/27522 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vicente, C.S.L., L.G. Nemchinov, M. Mota, J.D. Eisenback, K. Kamo, and P. Vieira. 2019. Identification and characterization of the first pectin methylesterase gene found in the root lesion nematode Pratylenchus penetrans. PLoS One. 2019 Feb 22;14(2):e0212540. doi: 10.1371/journal.pone.0212540. cvicente@uevora.pt 581 doi: 10.1371/journal.pone.0212540 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
PLoS |
publisher.none.fl_str_mv |
PLoS |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136657695309824 |