Influence of Ferredoxin, Iron-Sulfur Carrier and Cobalamin Importer Overexpression on the Production of High-value Products by Escherichia coli

• Autor(a) principal: Rebelo Manuel, Rita
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/130110
• topic: Cobalamin-dependent SAM enzymes; Ferredoxin; SufUT proteins; Btu proteins; Biotechnology for Sustainability

"One of the problems in antibiotics production is the difficulty of their synthesis or low yield in the case of complex biological molecules. They have complex biosynthetic pathways, where cobalamin-dependent SAM enzymes play a pivotal role, with an Fe-S cluster in their structure and the necessity of secondary electron transfer proteins for their activity, making the production improvement difficult. An example of these compounds are fosfomycin (Fom3 enzyme), a broadspectrum antibiotic, and thiostrepton (TsrM enzyme), a Gram-positive antibiotic with anticancer properties, both naturally produced by Streptomyces sp..(...)"