Platelet lysate-based pro-angiogenic nanocoatings

Detalhes bibliográficos
Autor(a) principal: Oliveira, Sara M.
Data de Publicação: 2016
Outros Autores: Pirraco, Rogério P., Marques, A. P., Santo, Vítor E., Gomes, Manuela E., Reis, R. L., Mano, J. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/40302
Resumo: Human platelet lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenicassociated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides. Statement of Significance The presence, or fast induction, of a stable and mature vasculature inside 3D constructs is crucial for new tissue formation and its viability. This has been one of the major tissue engineering challenges, limiting the dimensions of efficient tissue constructs. Many approaches based on cells, growth factors, 3D bioprinting and channel incorporation have been proposed. Herein, we explored a versatile technique, layer-by-layer assembling in combination with platelet lysate (PL), that is a cost-effective source of many potent pro-angiogenic proteins and growth factors. Results suggest that the combination of PL with sulfated polyelectrolytes might be used to introduce interfaces onto 2D/3D constructs with potential to induce the formation of cell-based tubular structures.
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spelling Platelet lysate-based pro-angiogenic nanocoatingsLayer-by-layer assemblingInstructive surfacesPlatelet lysateGrowth factorsAngiogenesisEndothelial cellsScience & TechnologyHuman platelet lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenicassociated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides. Statement of Significance The presence, or fast induction, of a stable and mature vasculature inside 3D constructs is crucial for new tissue formation and its viability. This has been one of the major tissue engineering challenges, limiting the dimensions of efficient tissue constructs. Many approaches based on cells, growth factors, 3D bioprinting and channel incorporation have been proposed. Herein, we explored a versatile technique, layer-by-layer assembling in combination with platelet lysate (PL), that is a cost-effective source of many potent pro-angiogenic proteins and growth factors. Results suggest that the combination of PL with sulfated polyelectrolytes might be used to introduce interfaces onto 2D/3D constructs with potential to induce the formation of cell-based tubular structures.The research leading to these results has received funding from European Union's Seventh Framework Program (FP7/2007-2013) under grant agreement na REGPOT-CT2012-316331 - POLARIS and FP7-KBBE-2010-4-266033 - SPECIAL. This work was also supported by the European Research Council grant agreement ERC-2012-ADG-20120216-321266 for the project ComplexiTE. Portuguese Foundation for Science and Technology is gratefully acknowledged for fellowship of Sara M. Oliveira (SFRH/BD/70107/2010). The researcher contract of R.P. Pirraco through RL3-TECT-NORTE-01-0124-FEDER-000020, co-financed by North Portugal Regional Operational Program (ON.2-O Novo Norte), under the National Strategic Reference Framework, through the European Regional Development Fund is also acknowledged.ElsevierUniversidade do MinhoOliveira, Sara M.Pirraco, Rogério P.Marques, A. P.Santo, Vítor E.Gomes, Manuela E.Reis, R. L.Mano, J. F.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40302engOliveira S. M., Pirraco R. P., Marques A. P., Santo V. E., Gomes M. E., Reis R. L., Mano J. F. Platelet lysate-based pro-angiogenic nanocoatings, Acta Biomaterialia, pp. in press, doi:10.1016/j.actbio.2015.12.028, 20151742-706110.1016/j.actbio.2015.12.02826708711http://www.sciencedirect.com/science/article/pii/S1742706115302658info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:41:34Zoai:repositorium.sdum.uminho.pt:1822/40302Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:38:34.048090Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Platelet lysate-based pro-angiogenic nanocoatings
title Platelet lysate-based pro-angiogenic nanocoatings
spellingShingle Platelet lysate-based pro-angiogenic nanocoatings
Oliveira, Sara M.
Layer-by-layer assembling
Instructive surfaces
Platelet lysate
Growth factors
Angiogenesis
Endothelial cells
Science & Technology
title_short Platelet lysate-based pro-angiogenic nanocoatings
title_full Platelet lysate-based pro-angiogenic nanocoatings
title_fullStr Platelet lysate-based pro-angiogenic nanocoatings
title_full_unstemmed Platelet lysate-based pro-angiogenic nanocoatings
title_sort Platelet lysate-based pro-angiogenic nanocoatings
author Oliveira, Sara M.
author_facet Oliveira, Sara M.
Pirraco, Rogério P.
Marques, A. P.
Santo, Vítor E.
Gomes, Manuela E.
Reis, R. L.
Mano, J. F.
author_role author
author2 Pirraco, Rogério P.
Marques, A. P.
Santo, Vítor E.
Gomes, Manuela E.
Reis, R. L.
Mano, J. F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, Sara M.
Pirraco, Rogério P.
Marques, A. P.
Santo, Vítor E.
Gomes, Manuela E.
Reis, R. L.
Mano, J. F.
dc.subject.por.fl_str_mv Layer-by-layer assembling
Instructive surfaces
Platelet lysate
Growth factors
Angiogenesis
Endothelial cells
Science & Technology
topic Layer-by-layer assembling
Instructive surfaces
Platelet lysate
Growth factors
Angiogenesis
Endothelial cells
Science & Technology
description Human platelet lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenicassociated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides. Statement of Significance The presence, or fast induction, of a stable and mature vasculature inside 3D constructs is crucial for new tissue formation and its viability. This has been one of the major tissue engineering challenges, limiting the dimensions of efficient tissue constructs. Many approaches based on cells, growth factors, 3D bioprinting and channel incorporation have been proposed. Herein, we explored a versatile technique, layer-by-layer assembling in combination with platelet lysate (PL), that is a cost-effective source of many potent pro-angiogenic proteins and growth factors. Results suggest that the combination of PL with sulfated polyelectrolytes might be used to introduce interfaces onto 2D/3D constructs with potential to induce the formation of cell-based tubular structures.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/40302
url http://hdl.handle.net/1822/40302
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oliveira S. M., Pirraco R. P., Marques A. P., Santo V. E., Gomes M. E., Reis R. L., Mano J. F. Platelet lysate-based pro-angiogenic nanocoatings, Acta Biomaterialia, pp. in press, doi:10.1016/j.actbio.2015.12.028, 2015
1742-7061
10.1016/j.actbio.2015.12.028
26708711
http://www.sciencedirect.com/science/article/pii/S1742706115302658
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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