Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.

Detalhes bibliográficos
Autor(a) principal: Sá-Nogueira, Isabel de
Data de Publicação: 2008
Outros Autores: Sanctis, Daniele de, Bento, Isabel, Inácio, José Manuel, Custódio, Sónia, Carrondo, Maria Arménia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/4202
Resumo: Acta Crystallographica F64 (2008) 636-638
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spelling Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.Acta Crystallographica F64 (2008) 636-638Two Bacillus subtilis extracellular endo-1,5-alpha-L-arabinanases, AbnA and Abn2, belonging to glycoside hydrolase family 43 have been identified. The recently characterized Abn2 protein hydrolyzes arabinan and has low identity to other reported 1,5-alpha-L-arabinanases. Abn2 and its selenomethionine (SeMet) derivative have been purified and crystallized. Crystals appeared in two different space groups: P1, with unit-cell parameters a = 51.9, b = 57.6, c = 86.2 A, alpha = 82.3, beta = 87.9, gamma = 63.6 degrees , and P2(1)2(1)2(1), with unit-cell parameters a = 57.9, b = 163.3, c = 202.0 A. X-ray data have been collected for the native and the SeMet derivative to 1.9 and 2.7 A resolution, respectively. An initial model of Abn2 is being built in the SeMet-phased map.This work was partially supported by grant No. POCI/AGR/60236/2004 from Fundacao para a Ciencia e Tecnologia (FCT) and FEDER to IdS-N and fellowship SFRH/BD/18238/2004 from FCT to JMI.International Union of CrystallographyRUNSá-Nogueira, Isabel deSanctis, Daniele deBento, IsabelInácio, José ManuelCustódio, SóniaCarrondo, Maria Arménia2010-10-25T11:20:11Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/4202engde Sanctis D, Bento I, Inácio JM, Custódio S, de Sá-Nogueira I, and Carrondo MA (2008). Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(Pt 7):636-638.1744-3091info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:33:53Zoai:run.unl.pt:10362/4202Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:15:35.679606Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.
title Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.
spellingShingle Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.
Sá-Nogueira, Isabel de
title_short Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.
title_full Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.
title_fullStr Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.
title_full_unstemmed Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.
title_sort Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis.
author Sá-Nogueira, Isabel de
author_facet Sá-Nogueira, Isabel de
Sanctis, Daniele de
Bento, Isabel
Inácio, José Manuel
Custódio, Sónia
Carrondo, Maria Arménia
author_role author
author2 Sanctis, Daniele de
Bento, Isabel
Inácio, José Manuel
Custódio, Sónia
Carrondo, Maria Arménia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Sá-Nogueira, Isabel de
Sanctis, Daniele de
Bento, Isabel
Inácio, José Manuel
Custódio, Sónia
Carrondo, Maria Arménia
description Acta Crystallographica F64 (2008) 636-638
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2010-10-25T11:20:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/4202
url http://hdl.handle.net/10362/4202
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv de Sanctis D, Bento I, Inácio JM, Custódio S, de Sá-Nogueira I, and Carrondo MA (2008). Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(Pt 7):636-638.
1744-3091
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv International Union of Crystallography
publisher.none.fl_str_mv International Union of Crystallography
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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