Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders
Main Author: | |
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Publication Date: | 2011 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/13441 |
Summary: | Psoralens are often used to treat skin disorders such as psoriasis, vitiligo and others. The toxicity and fast degradation of these drugs can be diminished by encapsulation in drug delivery systems (DDS). Nanoparticles (NPs) containing the benzopsoralen (BP) (3-ethoxy carbonyl-2H-benzofuro[3,2-e]-1-benzopiran-2-one) were prepared by the solvent evaporation technique, and parameters such as particle size, zeta potential, drug encapsulation efficiency, and external morphology were evaluated. The analysis revealed that the NPs are spherical and possessed a smooth external surface with diameter of 815 ± 80 nm, they present low tendency toward aggregation, as confirmed by their zeta potential (+17.3±2.9 mV) and the encapsulation efficiency obtained was 74%. The intracellular distribution of NPs as well as their uptake by tissues was monitored by using laser confocal microscopy and transmission electron microscopy. The use of benzopsoralen in association with ultraviolet light (360 nm) revealed morphological characteristics of cell damage such as cytosolic vesiculation, mitochondria condensation, and swelling of both the granular endoplasmic reticulum and the nuclear membrane. The primary target of DDS and drugs in vascular system are endothelial cells and an attractive strategy for modifying vascular function in various pathological states of skin disorders, cancer and inflammation. The result presented in this work indicates that PLGA NP could be a promising delivery system for benzopsoralen in connection with ultraviolet irradiation therapy (PUVA) for further application in different therapies. |
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Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disordersNanoparticlesBiological applications of polymersDrug-delivery systemMicroencapsulationTEMScience & TechnologyPsoralens are often used to treat skin disorders such as psoriasis, vitiligo and others. The toxicity and fast degradation of these drugs can be diminished by encapsulation in drug delivery systems (DDS). Nanoparticles (NPs) containing the benzopsoralen (BP) (3-ethoxy carbonyl-2H-benzofuro[3,2-e]-1-benzopiran-2-one) were prepared by the solvent evaporation technique, and parameters such as particle size, zeta potential, drug encapsulation efficiency, and external morphology were evaluated. The analysis revealed that the NPs are spherical and possessed a smooth external surface with diameter of 815 ± 80 nm, they present low tendency toward aggregation, as confirmed by their zeta potential (+17.3±2.9 mV) and the encapsulation efficiency obtained was 74%. The intracellular distribution of NPs as well as their uptake by tissues was monitored by using laser confocal microscopy and transmission electron microscopy. The use of benzopsoralen in association with ultraviolet light (360 nm) revealed morphological characteristics of cell damage such as cytosolic vesiculation, mitochondria condensation, and swelling of both the granular endoplasmic reticulum and the nuclear membrane. The primary target of DDS and drugs in vascular system are endothelial cells and an attractive strategy for modifying vascular function in various pathological states of skin disorders, cancer and inflammation. The result presented in this work indicates that PLGA NP could be a promising delivery system for benzopsoralen in connection with ultraviolet irradiation therapy (PUVA) for further application in different therapies.CNPq , CAPES, FAPESP (Braisl)WileyUniversidade do MinhoGomes, Anderson JLunardi, Laurelucia O.Caetano, Flavio H.Machado, António E. H.Campos, Ana M. F. OliveiraBendhack, Lusiane M.Lunardi, Claure N.2011-03-032011-03-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/13441eng1097-462810.1002/app.33427info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:39Zoai:repositorium.sdum.uminho.pt:1822/13441Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:21.705835Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders |
title |
Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders |
spellingShingle |
Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders Gomes, Anderson J Nanoparticles Biological applications of polymers Drug-delivery system Microencapsulation TEM Science & Technology |
title_short |
Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders |
title_full |
Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders |
title_fullStr |
Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders |
title_full_unstemmed |
Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders |
title_sort |
Biodegradable nanoparticles containing benzopsoralens : an attractive strategy for modifying vascular function in pathological skin disorders |
author |
Gomes, Anderson J |
author_facet |
Gomes, Anderson J Lunardi, Laurelucia O. Caetano, Flavio H. Machado, António E. H. Campos, Ana M. F. Oliveira Bendhack, Lusiane M. Lunardi, Claure N. |
author_role |
author |
author2 |
Lunardi, Laurelucia O. Caetano, Flavio H. Machado, António E. H. Campos, Ana M. F. Oliveira Bendhack, Lusiane M. Lunardi, Claure N. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gomes, Anderson J Lunardi, Laurelucia O. Caetano, Flavio H. Machado, António E. H. Campos, Ana M. F. Oliveira Bendhack, Lusiane M. Lunardi, Claure N. |
dc.subject.por.fl_str_mv |
Nanoparticles Biological applications of polymers Drug-delivery system Microencapsulation TEM Science & Technology |
topic |
Nanoparticles Biological applications of polymers Drug-delivery system Microencapsulation TEM Science & Technology |
description |
Psoralens are often used to treat skin disorders such as psoriasis, vitiligo and others. The toxicity and fast degradation of these drugs can be diminished by encapsulation in drug delivery systems (DDS). Nanoparticles (NPs) containing the benzopsoralen (BP) (3-ethoxy carbonyl-2H-benzofuro[3,2-e]-1-benzopiran-2-one) were prepared by the solvent evaporation technique, and parameters such as particle size, zeta potential, drug encapsulation efficiency, and external morphology were evaluated. The analysis revealed that the NPs are spherical and possessed a smooth external surface with diameter of 815 ± 80 nm, they present low tendency toward aggregation, as confirmed by their zeta potential (+17.3±2.9 mV) and the encapsulation efficiency obtained was 74%. The intracellular distribution of NPs as well as their uptake by tissues was monitored by using laser confocal microscopy and transmission electron microscopy. The use of benzopsoralen in association with ultraviolet light (360 nm) revealed morphological characteristics of cell damage such as cytosolic vesiculation, mitochondria condensation, and swelling of both the granular endoplasmic reticulum and the nuclear membrane. The primary target of DDS and drugs in vascular system are endothelial cells and an attractive strategy for modifying vascular function in various pathological states of skin disorders, cancer and inflammation. The result presented in this work indicates that PLGA NP could be a promising delivery system for benzopsoralen in connection with ultraviolet irradiation therapy (PUVA) for further application in different therapies. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-03 2011-03-03T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/13441 |
url |
http://hdl.handle.net/1822/13441 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1097-4628 10.1002/app.33427 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132976284434432 |