Characterization and genomic analysis of a new phage infecting Helicobacter pylori
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/78871 |
Resumo: | Helicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio)phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. pylori strains and UV radiation. One phage was isolated and further characterized to assess potential phage-inspired therapeutic alternatives to H. pylori infections. HPy1R is a new podovirus prophage with a genome length of 31,162 bp, 37.1% GC, encoding 36 predicted proteins, of which 17 were identified as structural. Phage particles remained stable at 37 °C, from pH 3 to 11, for 24 h in standard assays. Moreover, when submitted to an in vitro gastric digestion model, only a small decrease was observed in the gastric phase, suggesting that it is adapted to the gastric tract environment. Together with its other characteristics, its capability to suppress H. pylori population levels for up to 24 h post-infection at multiplicities of infection of 0.01, 0.1, and 1 suggests that this newly isolated phage is a potential candidate for phage therapy in the absence of strictly lytic phages. |
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Characterization and genomic analysis of a new phage infecting Helicobacter pyloriHelicobacter pyloriprophagephage therapygenomic analysisScience & TechnologyHelicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio)phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. pylori strains and UV radiation. One phage was isolated and further characterized to assess potential phage-inspired therapeutic alternatives to H. pylori infections. HPy1R is a new podovirus prophage with a genome length of 31,162 bp, 37.1% GC, encoding 36 predicted proteins, of which 17 were identified as structural. Phage particles remained stable at 37 °C, from pH 3 to 11, for 24 h in standard assays. Moreover, when submitted to an in vitro gastric digestion model, only a small decrease was observed in the gastric phase, suggesting that it is adapted to the gastric tract environment. Together with its other characteristics, its capability to suppress H. pylori population levels for up to 24 h post-infection at multiplicities of infection of 0.01, 0.1, and 1 suggests that this newly isolated phage is a potential candidate for phage therapy in the absence of strictly lytic phages.This study was supported by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of the UIDB/04469/2020 unit, and Project Helicophage PTDC/SAU-PUB/29182/2017 (POCI-01-0145-FEDER-029182). R.F. and R.F.S.G. acknowledge the FCT grants SFRH/BD/146496/2019 and SFRH/BD/140182/2018, respectivelyinfo:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoFerreira, Rute Vanessa NovaisSousa, Cláudia Sofia CunhaGonçalves, RaquelPinheiro, A. C.Oleastro, MónicaWagemans, JeroenLavigne, RobFigueiredo, CeuAzeredo, JoanaMelo, Luís Daniel Rodrigues2022-07-172022-07-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/78871engFerreira, R.; Sousa, Cláudia; Gonçalves, Raquel F. S.; Pinheiro, Ana Cristina; Oleastro, Mónica; Wagemans, Jeroen; Lavigne, Rob; Figueiredo, Ceu; Azeredo, Joana; Melo, Luís Daniel Rodrigues, Characterization and genomic analysis of a new phage infecting Helicobacter pylori. International Journal of Molecular Sciences, 23(14), 7885, 20221424-67831422006710.3390/ijms2314788535887231https://www.mdpi.com/1422-0067/23/14/7885info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:54:25Zoai:repositorium.sdum.uminho.pt:1822/78871Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:59.133628Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization and genomic analysis of a new phage infecting Helicobacter pylori |
title |
Characterization and genomic analysis of a new phage infecting Helicobacter pylori |
spellingShingle |
Characterization and genomic analysis of a new phage infecting Helicobacter pylori Ferreira, Rute Vanessa Novais Helicobacter pylori prophage phage therapy genomic analysis Science & Technology |
title_short |
Characterization and genomic analysis of a new phage infecting Helicobacter pylori |
title_full |
Characterization and genomic analysis of a new phage infecting Helicobacter pylori |
title_fullStr |
Characterization and genomic analysis of a new phage infecting Helicobacter pylori |
title_full_unstemmed |
Characterization and genomic analysis of a new phage infecting Helicobacter pylori |
title_sort |
Characterization and genomic analysis of a new phage infecting Helicobacter pylori |
author |
Ferreira, Rute Vanessa Novais |
author_facet |
Ferreira, Rute Vanessa Novais Sousa, Cláudia Sofia Cunha Gonçalves, Raquel Pinheiro, A. C. Oleastro, Mónica Wagemans, Jeroen Lavigne, Rob Figueiredo, Ceu Azeredo, Joana Melo, Luís Daniel Rodrigues |
author_role |
author |
author2 |
Sousa, Cláudia Sofia Cunha Gonçalves, Raquel Pinheiro, A. C. Oleastro, Mónica Wagemans, Jeroen Lavigne, Rob Figueiredo, Ceu Azeredo, Joana Melo, Luís Daniel Rodrigues |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ferreira, Rute Vanessa Novais Sousa, Cláudia Sofia Cunha Gonçalves, Raquel Pinheiro, A. C. Oleastro, Mónica Wagemans, Jeroen Lavigne, Rob Figueiredo, Ceu Azeredo, Joana Melo, Luís Daniel Rodrigues |
dc.subject.por.fl_str_mv |
Helicobacter pylori prophage phage therapy genomic analysis Science & Technology |
topic |
Helicobacter pylori prophage phage therapy genomic analysis Science & Technology |
description |
Helicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio)phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. pylori strains and UV radiation. One phage was isolated and further characterized to assess potential phage-inspired therapeutic alternatives to H. pylori infections. HPy1R is a new podovirus prophage with a genome length of 31,162 bp, 37.1% GC, encoding 36 predicted proteins, of which 17 were identified as structural. Phage particles remained stable at 37 °C, from pH 3 to 11, for 24 h in standard assays. Moreover, when submitted to an in vitro gastric digestion model, only a small decrease was observed in the gastric phase, suggesting that it is adapted to the gastric tract environment. Together with its other characteristics, its capability to suppress H. pylori population levels for up to 24 h post-infection at multiplicities of infection of 0.01, 0.1, and 1 suggests that this newly isolated phage is a potential candidate for phage therapy in the absence of strictly lytic phages. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-17 2022-07-17T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/78871 |
url |
https://hdl.handle.net/1822/78871 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ferreira, R.; Sousa, Cláudia; Gonçalves, Raquel F. S.; Pinheiro, Ana Cristina; Oleastro, Mónica; Wagemans, Jeroen; Lavigne, Rob; Figueiredo, Ceu; Azeredo, Joana; Melo, Luís Daniel Rodrigues, Characterization and genomic analysis of a new phage infecting Helicobacter pylori. International Journal of Molecular Sciences, 23(14), 7885, 2022 1424-6783 14220067 10.3390/ijms23147885 35887231 https://www.mdpi.com/1422-0067/23/14/7885 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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