Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori

Detalhes bibliográficos
Autor(a) principal: Ferreira, Rute
Data de Publicação: 2022
Outros Autores: Sousa, Cláudia, Gonçalves, Raquel F.S., Pinheiro, Ana Cristina, Oleastro, Mónica, Wagemans, Jeroen, Lavigne, Rob, Figueiredo, Ceu, Azeredo, Joana, Melo, Luís D.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8610
Resumo: This article belongs to the Special Issue Helicobacter: Infection, Diagnosis and Treatment 2.0.
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spelling Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pyloriHelicobacter pyloriProphagePhage TherapyGenomic AnalysisInfecções GastrointestinaisThis article belongs to the Special Issue Helicobacter: Infection, Diagnosis and Treatment 2.0.Helicobacter pylori, a significant human gastric pathogen, has been demonstrating increased antibiotic resistance, causing difficulties in infection treatment. It is therefore important to develop alternatives or complementary approaches to antibiotics to tackle H. pylori infections, and (bacterio) phages have proven to be effective antibacterial agents. In this work, prophage isolation was attempted using H. pylori strains and UV radiation. One phage was isolated and further characterized to assess potential phage-inspired therapeutic alternatives to H. pylori infections. HPy1R is a new podovirus prophage with a genome length of 31,162 bp, 37.1% GC, encoding 36 predicted proteins, of which 17 were identified as structural. Phage particles remained stable at 37 C, from pH 3 to 11, for 24 h in standard assays. Moreover, when submitted to an in vitro gastric digestion model, only a small decrease was observed in the gastric phase, suggesting that it is adapted to the gastric tract environment. Together with its other characteristics, its capability to suppress H. pylori population levels for up to 24 h post-infection at multiplicities of infection of 0.01, 0.1, and 1 suggests that this newly isolated phage is a potential candidate for phage therapy in the absence of strictly lytic phages.This study was supported by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of the UIDB/04469/2020 unit, and Project Helicophage PTDC/SAU-PUB/29182/2017 (POCI-01-0145-FEDER-029182). R.F. and R.F.S.G. acknowledge the FCT grants SFRH/BD/146496/2019 and SFRH/BD/140182/2018, respectively.MDPIRepositório Científico do Instituto Nacional de SaúdeFerreira, RuteSousa, CláudiaGonçalves, Raquel F.S.Pinheiro, Ana CristinaOleastro, MónicaWagemans, JeroenLavigne, RobFigueiredo, CeuAzeredo, JoanaMelo, Luís D.R.2023-04-10T14:37:12Z2022-07-172022-07-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8610engInt J Mol Sci. 2022 Jul 17;23(14):7885. doi: 10.3390/ijms231478851661-659610.3390/ijms23147885info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:37Zoai:repositorio.insa.pt:10400.18/8610Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:10.055979Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
title Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
spellingShingle Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
Ferreira, Rute
Helicobacter pylori
Prophage
Phage Therapy
Genomic Analysis
Infecções Gastrointestinais
title_short Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
title_full Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
title_fullStr Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
title_full_unstemmed Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
title_sort Characterization and Genomic Analysis of a New Phage Infecting Helicobacter pylori
author Ferreira, Rute
author_facet Ferreira, Rute
Sousa, Cláudia
Gonçalves, Raquel F.S.
Pinheiro, Ana Cristina
Oleastro, Mónica
Wagemans, Jeroen
Lavigne, Rob
Figueiredo, Ceu
Azeredo, Joana
Melo, Luís D.R.
author_role author
author2 Sousa, Cláudia
Gonçalves, Raquel F.S.
Pinheiro, Ana Cristina
Oleastro, Mónica
Wagemans, Jeroen
Lavigne, Rob
Figueiredo, Ceu
Azeredo, Joana
Melo, Luís D.R.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Ferreira, Rute
Sousa, Cláudia
Gonçalves, Raquel F.S.
Pinheiro, Ana Cristina
Oleastro, Mónica
Wagemans, Jeroen
Lavigne, Rob
Figueiredo, Ceu
Azeredo, Joana
Melo, Luís D.R.
dc.subject.por.fl_str_mv Helicobacter pylori
Prophage
Phage Therapy
Genomic Analysis
Infecções Gastrointestinais
topic Helicobacter pylori
Prophage
Phage Therapy
Genomic Analysis
Infecções Gastrointestinais
description This article belongs to the Special Issue Helicobacter: Infection, Diagnosis and Treatment 2.0.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-17
2022-07-17T00:00:00Z
2023-04-10T14:37:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8610
url http://hdl.handle.net/10400.18/8610
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Mol Sci. 2022 Jul 17;23(14):7885. doi: 10.3390/ijms23147885
1661-6596
10.3390/ijms23147885
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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