Establishment of a genomic DNA extraction protocol for bamboo species
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.19084/RCA17336 |
Resumo: | The bamboos Bambusa vulgaris and Guadua angustifolia are considered the most important species in South America. Generally, bamboo taxonomy is conducted by analysis of plants morphology, which sometimes may present morphological similarities, complicating the achievement of precise results. This fact can be changed by application of molecular biology methods; however, the Brazilian research of bamboo in molecular level is still limited. The Brazilian state of Mato Grosso do Sul has fragments of a natural bamboo forest with external characteristics similar to Guadua. In order to provide information about bamboo species, this paper evaluates the application of a modified protocol for DNA extraction from young leaves of two accesses of Guadua cultivated in Campo Grande/MS. It also evaluates the amplification efficiency of the matK and rbcL regions of the DNA isolated. It was possible to extract a mean of 487.5 ng μL-1 of DNA for both species with a purity level of 1.91. The molecular marker rbcL amplified the regions of 300 bp for both species, while the marker matK amplified the regions of 850 bp, also for both accesses. Results show that the modified protocol is useful for bamboos DNA extraction, with high concentration, quality, and able to be amplified by PCR. |
id |
RCAP_bfc23727dc0c1a3a30ee78537df614f8 |
---|---|
oai_identifier_str |
oai:ojs.revistas.rcaap.pt:article/16749 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Establishment of a genomic DNA extraction protocol for bamboo speciesEstabelecimento de um protocolo de extração de DNA genómico para espécies de bambuGeralThe bamboos Bambusa vulgaris and Guadua angustifolia are considered the most important species in South America. Generally, bamboo taxonomy is conducted by analysis of plants morphology, which sometimes may present morphological similarities, complicating the achievement of precise results. This fact can be changed by application of molecular biology methods; however, the Brazilian research of bamboo in molecular level is still limited. The Brazilian state of Mato Grosso do Sul has fragments of a natural bamboo forest with external characteristics similar to Guadua. In order to provide information about bamboo species, this paper evaluates the application of a modified protocol for DNA extraction from young leaves of two accesses of Guadua cultivated in Campo Grande/MS. It also evaluates the amplification efficiency of the matK and rbcL regions of the DNA isolated. It was possible to extract a mean of 487.5 ng μL-1 of DNA for both species with a purity level of 1.91. The molecular marker rbcL amplified the regions of 300 bp for both species, while the marker matK amplified the regions of 850 bp, also for both accesses. Results show that the modified protocol is useful for bamboos DNA extraction, with high concentration, quality, and able to be amplified by PCR.Os bambus Bambusa vulgaris e Guadua angustifolia são considerados as espécies mais importantes na América do Sul. Geralmente, a taxonômia de bambus é realizada pela análise da morfologia das plantas, que algumas vezes apresentam similaridades morfológicas, dificultando a obtenção de resultados precisos. Isso pode ser mudado pelo uso de métodos de biologia molecular, no entanto as pesquisas brasileiras com bamboo a nível molecular ainda é limitada. O estado do Mato Grosso do Sul possui fragmentos de florestas de bambu com características semelhantes ao Guadua. Com o intuito de fornecer informações sobre espécies de bambu, o presente trabalho avalia a aplicação de um protocolo modificado de extração de DNA de folhas jovens de duas amostras de Guaduacultivados em Campo Grande/MS. Também é avaliado a eficiência na amplificação das regiões matK e rbcL do DNA isolado. Foi possível extrair em média 487,5 ng μL-1 de DNA para ambas as espécies com nível de pureza de 1,91. O marcador molecular rbcL amplificou a região de 300 pb, enquanto que o marcador matK amplificou a região de 850 pb, ambos para os dois acessos. Resultados mostram que o protocolo modificado é útil para a extração de DNA de bambus, com alta quantidade, qualidade, e capaz de ser amplificado por PCR.Sociedade de Ciências Agrárias de Portugal2019-01-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://doi.org/10.19084/RCA17336eng2183-041X0871-018XSilva, Rudieli M.Ribeiro, Nathalia P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T09:24:51Zoai:ojs.revistas.rcaap.pt:article/16749Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:31:04.208454Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Establishment of a genomic DNA extraction protocol for bamboo species Estabelecimento de um protocolo de extração de DNA genómico para espécies de bambu |
title |
Establishment of a genomic DNA extraction protocol for bamboo species |
spellingShingle |
Establishment of a genomic DNA extraction protocol for bamboo species Silva, Rudieli M. Geral |
title_short |
Establishment of a genomic DNA extraction protocol for bamboo species |
title_full |
Establishment of a genomic DNA extraction protocol for bamboo species |
title_fullStr |
Establishment of a genomic DNA extraction protocol for bamboo species |
title_full_unstemmed |
Establishment of a genomic DNA extraction protocol for bamboo species |
title_sort |
Establishment of a genomic DNA extraction protocol for bamboo species |
author |
Silva, Rudieli M. |
author_facet |
Silva, Rudieli M. Ribeiro, Nathalia P. |
author_role |
author |
author2 |
Ribeiro, Nathalia P. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Silva, Rudieli M. Ribeiro, Nathalia P. |
dc.subject.por.fl_str_mv |
Geral |
topic |
Geral |
description |
The bamboos Bambusa vulgaris and Guadua angustifolia are considered the most important species in South America. Generally, bamboo taxonomy is conducted by analysis of plants morphology, which sometimes may present morphological similarities, complicating the achievement of precise results. This fact can be changed by application of molecular biology methods; however, the Brazilian research of bamboo in molecular level is still limited. The Brazilian state of Mato Grosso do Sul has fragments of a natural bamboo forest with external characteristics similar to Guadua. In order to provide information about bamboo species, this paper evaluates the application of a modified protocol for DNA extraction from young leaves of two accesses of Guadua cultivated in Campo Grande/MS. It also evaluates the amplification efficiency of the matK and rbcL regions of the DNA isolated. It was possible to extract a mean of 487.5 ng μL-1 of DNA for both species with a purity level of 1.91. The molecular marker rbcL amplified the regions of 300 bp for both species, while the marker matK amplified the regions of 850 bp, also for both accesses. Results show that the modified protocol is useful for bamboos DNA extraction, with high concentration, quality, and able to be amplified by PCR. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-18T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.19084/RCA17336 |
url |
https://doi.org/10.19084/RCA17336 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2183-041X 0871-018X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799130176818249728 |