Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)

Detalhes bibliográficos
Autor(a) principal: Waldschmidt,Ana Maria
Data de Publicação: 1997
Outros Autores: Salomão,Tânia Maria Fernandes, Barros,Everaldo Gonçalves de, Campos,Lúcio de Antônio Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Genetics
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300011
Resumo: The objective of the present study was to test three different procedures for DNA extraction of Melipona quadrifasciata based on existing methods for DNA extraction of Apis, plants and fungi. These methods differ in the concentrations of specific substances in the extraction buffer. The results demonstrate that the method used for Apis is not adequate for DNA extraction from M. quadrifasciata. On the other hand, with minor modifications this method and the methods for plants and fungi were adequate for DNA extraction of this stingless bee, both for adults and larvae
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spelling Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)The objective of the present study was to test three different procedures for DNA extraction of Melipona quadrifasciata based on existing methods for DNA extraction of Apis, plants and fungi. These methods differ in the concentrations of specific substances in the extraction buffer. The results demonstrate that the method used for Apis is not adequate for DNA extraction from M. quadrifasciata. On the other hand, with minor modifications this method and the methods for plants and fungi were adequate for DNA extraction of this stingless bee, both for adults and larvaeSociedade Brasileira de Genética1997-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300011Brazilian Journal of Genetics v.20 n.3 1997reponame:Brazilian Journal of Geneticsinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S0100-84551997000300011info:eu-repo/semantics/openAccessWaldschmidt,Ana MariaSalomão,Tânia Maria FernandesBarros,Everaldo Gonçalves deCampos,Lúcio de Antônio Oliveiraeng1998-10-06T00:00:00Zoai:scielo:S0100-84551997000300011Revistahttps://www.gmb.org.br/brazilian-journal-of-geneticsONGhttps://old.scielo.br/oai/scielo-oai.phpsede@sgb.org.br || sede@sgb.org.br0100-84550100-8455opendoar:1998-10-06T00:00Brazilian Journal of Genetics - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
title Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
spellingShingle Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
Waldschmidt,Ana Maria
title_short Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
title_full Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
title_fullStr Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
title_full_unstemmed Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
title_sort Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
author Waldschmidt,Ana Maria
author_facet Waldschmidt,Ana Maria
Salomão,Tânia Maria Fernandes
Barros,Everaldo Gonçalves de
Campos,Lúcio de Antônio Oliveira
author_role author
author2 Salomão,Tânia Maria Fernandes
Barros,Everaldo Gonçalves de
Campos,Lúcio de Antônio Oliveira
author2_role author
author
author
dc.contributor.author.fl_str_mv Waldschmidt,Ana Maria
Salomão,Tânia Maria Fernandes
Barros,Everaldo Gonçalves de
Campos,Lúcio de Antônio Oliveira
description The objective of the present study was to test three different procedures for DNA extraction of Melipona quadrifasciata based on existing methods for DNA extraction of Apis, plants and fungi. These methods differ in the concentrations of specific substances in the extraction buffer. The results demonstrate that the method used for Apis is not adequate for DNA extraction from M. quadrifasciata. On the other hand, with minor modifications this method and the methods for plants and fungi were adequate for DNA extraction of this stingless bee, both for adults and larvae
publishDate 1997
dc.date.none.fl_str_mv 1997-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-84551997000300011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Brazilian Journal of Genetics v.20 n.3 1997
reponame:Brazilian Journal of Genetics
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Brazilian Journal of Genetics
collection Brazilian Journal of Genetics
repository.name.fl_str_mv Brazilian Journal of Genetics - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv sede@sgb.org.br || sede@sgb.org.br
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