Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract

Detalhes bibliográficos
Autor(a) principal: Marques, Filipe
Data de Publicação: 2011
Outros Autores: Azevedo, F., Johansson, Björn, Oliveira, Rui Pedro Soares de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/15868
Resumo: Many extracts prepared from plants traditionally used for medicinal applications contain a variety of phytochemicals with antioxidant and antigenotoxic activity. In this work we measured the DNA protective effect of extracts of Ginkgo biloba leaves from oxidative stress using Saccharomyces cerevisiae as experimental model. The extract improved viability of yeast cells under oxidative stress imposed by hydrogen peroxide. In accordance with previous reports on antioxidant properties of G. biloba extracts, pre-incubation of yeast cells promoted a decrease in intracellular oxidation. We assessed DNA damage by our recently developed yeast comet assay protocol. Upon oxidative shock, DNA damage decreased in a dose-dependent manner in experiments of pre-incubation and simultaneous incubation with the extract, indicating a direct protective effect. In addition, the extract improved DNA repair rate following oxidative shock as measured by faster disappearance of comet tails. This suggests that the extract stimulates the DNA repair machinery in its DNA protective action in addition to directly protect DNA from oxidation. The observed DNA repair depends on the DNA repair machinery since no DNA repair was observed under restrictive conditions in a conditional mutant of the CDC9 gene (Accession No. Z74212), encoding the DNA ligase involved in the final step of both nucleotide and base excision repair.
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spelling Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extractOxidative stressAntigenotoxicAntioxidantYeast comet assayGinkgo bilobaSaccharomyces cerevisiaeScience & TechnologyMany extracts prepared from plants traditionally used for medicinal applications contain a variety of phytochemicals with antioxidant and antigenotoxic activity. In this work we measured the DNA protective effect of extracts of Ginkgo biloba leaves from oxidative stress using Saccharomyces cerevisiae as experimental model. The extract improved viability of yeast cells under oxidative stress imposed by hydrogen peroxide. In accordance with previous reports on antioxidant properties of G. biloba extracts, pre-incubation of yeast cells promoted a decrease in intracellular oxidation. We assessed DNA damage by our recently developed yeast comet assay protocol. Upon oxidative shock, DNA damage decreased in a dose-dependent manner in experiments of pre-incubation and simultaneous incubation with the extract, indicating a direct protective effect. In addition, the extract improved DNA repair rate following oxidative shock as measured by faster disappearance of comet tails. This suggests that the extract stimulates the DNA repair machinery in its DNA protective action in addition to directly protect DNA from oxidation. The observed DNA repair depends on the DNA repair machinery since no DNA repair was observed under restrictive conditions in a conditional mutant of the CDC9 gene (Accession No. Z74212), encoding the DNA ligase involved in the final step of both nucleotide and base excision repair.ElsevierUniversidade do MinhoMarques, FilipeAzevedo, F.Johansson, BjörnOliveira, Rui Pedro Soares de2011-062011-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/15868eng0278-691510.1016/j.fct.2011.03.02021419822The original publication is available at http://www.sciencedirect.com/science/article/pii/S0278691511000895info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:18Zoai:repositorium.sdum.uminho.pt:1822/15868Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:07.889707Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract
title Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract
spellingShingle Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract
Marques, Filipe
Oxidative stress
Antigenotoxic
Antioxidant
Yeast comet assay
Ginkgo biloba
Saccharomyces cerevisiae
Science & Technology
title_short Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract
title_full Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract
title_fullStr Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract
title_full_unstemmed Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract
title_sort Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf extract
author Marques, Filipe
author_facet Marques, Filipe
Azevedo, F.
Johansson, Björn
Oliveira, Rui Pedro Soares de
author_role author
author2 Azevedo, F.
Johansson, Björn
Oliveira, Rui Pedro Soares de
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Marques, Filipe
Azevedo, F.
Johansson, Björn
Oliveira, Rui Pedro Soares de
dc.subject.por.fl_str_mv Oxidative stress
Antigenotoxic
Antioxidant
Yeast comet assay
Ginkgo biloba
Saccharomyces cerevisiae
Science & Technology
topic Oxidative stress
Antigenotoxic
Antioxidant
Yeast comet assay
Ginkgo biloba
Saccharomyces cerevisiae
Science & Technology
description Many extracts prepared from plants traditionally used for medicinal applications contain a variety of phytochemicals with antioxidant and antigenotoxic activity. In this work we measured the DNA protective effect of extracts of Ginkgo biloba leaves from oxidative stress using Saccharomyces cerevisiae as experimental model. The extract improved viability of yeast cells under oxidative stress imposed by hydrogen peroxide. In accordance with previous reports on antioxidant properties of G. biloba extracts, pre-incubation of yeast cells promoted a decrease in intracellular oxidation. We assessed DNA damage by our recently developed yeast comet assay protocol. Upon oxidative shock, DNA damage decreased in a dose-dependent manner in experiments of pre-incubation and simultaneous incubation with the extract, indicating a direct protective effect. In addition, the extract improved DNA repair rate following oxidative shock as measured by faster disappearance of comet tails. This suggests that the extract stimulates the DNA repair machinery in its DNA protective action in addition to directly protect DNA from oxidation. The observed DNA repair depends on the DNA repair machinery since no DNA repair was observed under restrictive conditions in a conditional mutant of the CDC9 gene (Accession No. Z74212), encoding the DNA ligase involved in the final step of both nucleotide and base excision repair.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
2011-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/15868
url http://hdl.handle.net/1822/15868
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0278-6915
10.1016/j.fct.2011.03.020
21419822
The original publication is available at http://www.sciencedirect.com/science/article/pii/S0278691511000895
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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