Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/142146 |
Resumo: | Funding: This work was supported by an operating grant from the Canadian Institutes of Health Research to W. W. Lautt. P. Sadri was supported by an NSERC Postdoctoral Fellowship. R. A. Afonso was supported by a PhD Fellowship (SFRH/BD/ 9082/2002) from the Foundation for Science and Technology (FCT). |
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Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucroseFeedingGlucoseHISSInsulin sensitivityMealRISTSucroseMedicine (miscellaneous)Funding: This work was supported by an operating grant from the Canadian Institutes of Health Research to W. W. Lautt. P. Sadri was supported by an NSERC Postdoctoral Fellowship. R. A. Afonso was supported by a PhD Fellowship (SFRH/BD/ 9082/2002) from the Foundation for Science and Technology (FCT).We have recently shown that meal-induced insulin sensitization (MIS) occurs after feeding and decreases progressively to insignificance after 24 h of fasting and is caused by action of a hepatic insulin sensitizing substance (HISS). In order to carry out quantitative studies of MIS, some standardized meal intake is required. Our objective was to establish animal models to be tested in both the conscious and anaesthetized state using intragastric injection of liquid meals in order to quantify MIS. Insulin sensitivity was assessed before and 90 min after the meal using the rapid insulin sensitivity test (RIST) which is a transient euglycaemic clamp. Rats tested in the conscious state were instrumented under anaesthesia 6-9 d prior to testing with catheters in the carotid artery, jugular vein and stomach. Meals, injected into the stomach, consisted of a liquid mixed meal, sucrose, glucose or water. The glucose sequestration in response to insulin increased by 90 % and 61 % following the liquid mixed meal (10 ml/kg) in conscious and anaesthetized rats, respectively. Glucose, sucrose and water did not effectively activate MIS. MIS was completely reversed in the conscious model by atropine and completely prevented from developing in the anaesthetized model that had previously undergone hepatic denervation. Gastric administration of a liquid mixed meal but not glucose or sucrose is capable of activating MIS for purposes of mechanistic studies and quantification of the MIS process. The feeding signal is mediated by the hepatic parasympathetic nerves.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNSadri, ParissaReid, Maria A GAfonso, Ricardo A.Schafer, JoshuaLegare, Dallas J.Macedo, M. PaulaLautt, W. Wayne2024-01-24T01:31:44Z2006-022006-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/10362/142146eng0007-1145PURE: 2923420https://doi.org/10.1079/BJN20051644info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:19:35Zoai:run.unl.pt:10362/142146Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:09.935785Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose |
| title |
Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose |
| spellingShingle |
Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose Sadri, Parissa Feeding Glucose HISS Insulin sensitivity Meal RIST Sucrose Medicine (miscellaneous) |
| title_short |
Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose |
| title_full |
Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose |
| title_fullStr |
Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose |
| title_full_unstemmed |
Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose |
| title_sort |
Meal-induced insulin sensitization in conscious and anaesthetized rat models comparing liquid mixed meal with glucose and sucrose |
| author |
Sadri, Parissa |
| author_facet |
Sadri, Parissa Reid, Maria A G Afonso, Ricardo A. Schafer, Joshua Legare, Dallas J. Macedo, M. Paula Lautt, W. Wayne |
| author_role |
author |
| author2 |
Reid, Maria A G Afonso, Ricardo A. Schafer, Joshua Legare, Dallas J. Macedo, M. Paula Lautt, W. Wayne |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
| dc.contributor.author.fl_str_mv |
Sadri, Parissa Reid, Maria A G Afonso, Ricardo A. Schafer, Joshua Legare, Dallas J. Macedo, M. Paula Lautt, W. Wayne |
| dc.subject.por.fl_str_mv |
Feeding Glucose HISS Insulin sensitivity Meal RIST Sucrose Medicine (miscellaneous) |
| topic |
Feeding Glucose HISS Insulin sensitivity Meal RIST Sucrose Medicine (miscellaneous) |
| description |
Funding: This work was supported by an operating grant from the Canadian Institutes of Health Research to W. W. Lautt. P. Sadri was supported by an NSERC Postdoctoral Fellowship. R. A. Afonso was supported by a PhD Fellowship (SFRH/BD/ 9082/2002) from the Foundation for Science and Technology (FCT). |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-02 2006-02-01T00:00:00Z 2024-01-24T01:31:44Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/142146 |
| url |
http://hdl.handle.net/10362/142146 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0007-1145 PURE: 2923420 https://doi.org/10.1079/BJN20051644 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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8 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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