Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates

Detalhes bibliográficos
Autor(a) principal: González-Díaz, Aida
Data de Publicação: 2019
Outros Autores: Tubau, Fe, Pinto, Miguel, Sierra, Yanik, Cubero, Meritxell, Càmara, Jordi, Ayats, Josefina, Bajanca-Lavado, Paula, Ardanuy, Carmen, Marti, Sara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6519
Resumo: Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30872664/
id RCAP_c199cb9a15fcbf3ad89c813bf929653c
oai_identifier_str oai:repositorio.insa.pt:10400.18/6519
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolatesHaemophilus parainfluenzaeExtensively Drug-resistatGenitourinary TractCapsuleInfecções RespiratóriasFree PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30872664/The human commensal Haemophilus parainfluenzae is emerging as an opportunistic multidrug-resistant pathogen. The objectives of this work were to characterise a new capsular operon of extensively drug-resistant (XDR) H. parainfluenzae clinical isolates and study their resistance mechanisms using whole-genome sequencing. All strains were resistant to: ß-lactams, via amino acid changes in PBP3 (S385T, I442F, V511A, N526K and V562I); quinolones, by alterations in GyrA (S84F and D88Y) and ParC (S84F and S138T); chloramphenicol, through the presence of catS; macrolides, via the presence of mel and mef(E)-carrying MEGA element; and tetracycline, through the presence of tet(M) and/or tet(B). Phylogenetic analysis revealed high genomic diversity when compared to the H. parainfluenzae genomes available on the NCBI, the isolates from this study being closely related to the Swiss XDR AE-2096513. A full capsular operon showing homology to that of H. influenzae was identified, in accordance with the observation of a capsular structure by TEM. This study describes for the first time a capsular operon in H. parainfluenzae, a major determinant of pathogenicity that may contribute to increased virulence in XDR clinical isolates. Moreover, phylogenetic analysis suggests the possible spread of an XDR-encapsulated strain in Europe.This study has been funded by Instituto de Salud Carlos III through the Projects from the Fondo de Investigaciones Sanitarias “PI16/00977” and CIBER de Enfermedades Respiratorias (CIBERES - CB06/06/0037), and Co-funded by the European Regional Development Fund/European Social Fund (ERDF/ESF, “Investing in your future”).Nature ResearchRepositório Científico do Instituto Nacional de SaúdeGonzález-Díaz, AidaTubau, FePinto, MiguelSierra, YanikCubero, MeritxellCàmara, JordiAyats, JosefinaBajanca-Lavado, PaulaArdanuy, CarmenMarti, Sara2020-04-25T11:17:01Z2019-03-142019-03-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6519engSci Rep . 2019 Mar 14;9(1):4481. doi: 10.1038/s41598-019-40812-22045-232210.1038/s41598-019-40812-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:42Zoai:repositorio.insa.pt:10400.18/6519Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:33.752344Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
title Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
spellingShingle Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
González-Díaz, Aida
Haemophilus parainfluenzae
Extensively Drug-resistat
Genitourinary Tract
Capsule
Infecções Respiratórias
title_short Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
title_full Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
title_fullStr Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
title_full_unstemmed Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
title_sort Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
author González-Díaz, Aida
author_facet González-Díaz, Aida
Tubau, Fe
Pinto, Miguel
Sierra, Yanik
Cubero, Meritxell
Càmara, Jordi
Ayats, Josefina
Bajanca-Lavado, Paula
Ardanuy, Carmen
Marti, Sara
author_role author
author2 Tubau, Fe
Pinto, Miguel
Sierra, Yanik
Cubero, Meritxell
Càmara, Jordi
Ayats, Josefina
Bajanca-Lavado, Paula
Ardanuy, Carmen
Marti, Sara
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv González-Díaz, Aida
Tubau, Fe
Pinto, Miguel
Sierra, Yanik
Cubero, Meritxell
Càmara, Jordi
Ayats, Josefina
Bajanca-Lavado, Paula
Ardanuy, Carmen
Marti, Sara
dc.subject.por.fl_str_mv Haemophilus parainfluenzae
Extensively Drug-resistat
Genitourinary Tract
Capsule
Infecções Respiratórias
topic Haemophilus parainfluenzae
Extensively Drug-resistat
Genitourinary Tract
Capsule
Infecções Respiratórias
description Free PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30872664/
publishDate 2019
dc.date.none.fl_str_mv 2019-03-14
2019-03-14T00:00:00Z
2020-04-25T11:17:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6519
url http://hdl.handle.net/10400.18/6519
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sci Rep . 2019 Mar 14;9(1):4481. doi: 10.1038/s41598-019-40812-2
2045-2322
10.1038/s41598-019-40812-2
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132160726138880

Registros relacionados