Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM

Detalhes bibliográficos
Autor(a) principal: Pinho, MD
Data de Publicação: 2019
Outros Autores: Foster, G, Pomba, C., Machado, MP, Baily, JL, Kuiken, T, Melo-Cristino, J, Ramirez, M, Vaz, T, Giao, M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22242
Resumo: Research Areas: Microbiology
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spelling Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCMStreptococcus canismultilocus sequence typingM-like protein (SCM) genewildlifegenomeResearch Areas: MicrobiologyABSTRACT - Streptococcus canis is an animal pathogen which occasionally causes infections in humans. The S. canis M-like protein (SCM) encoded by the scm gene, is its best characterized virulence factor but previous studies suggested it could be absent in a substantial fraction of isolates. We studied the distribution and variability of the scm gene in 188 S. canis isolates recovered from companion animals (n = 152), wild animal species (n = 20), and humans (n = 14). Multilocus sequence typing, including the first characterization of wildlife isolates, showed that the same lineages are present in all animal hosts, raising the possibility of extensive circulation between species. Whole-genome analysis revealed that emm-like genes found previously in S. canis correspond to divergent scm genes, indicating that what was previously believed to correspond to two genes is in fact the same scm locus. We designed primers allowing for the first time the successful amplification of the scm gene in all isolates. Analysis of the scm sequences identified 12 distinct types, which could be divided into two clusters: group I (76%, n = 142) and group II (24%, n = 46) sharing little sequence similarity. The predicted group I SCM showed extensive similarity with each other outside of the N-terminal hypervariable region and a conserved IgG binding domain. This domain was absent from group II SCM variants found in isolates previously thought to lack the scm gene, which also showed greater amino acid variability. Further studies are necessary to elucidate the possible host interacting partners of the group II SCM variants and their role in virulence.Frontiers MediaRepositório da Universidade de LisboaPinho, MDFoster, GPomba, C.Machado, MPBaily, JLKuiken, TMelo-Cristino, JRamirez, MVaz, TGiao, M2021-10-13T23:04:47Z2019-03-292019-03-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22242engPinho MD, Foster G, Pomba C, Machado MP, Baily JL, Kuiken T, Melo-Cristino J, Ramirez M et al. 2019. Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM. Frontiers in Microbiology 10:6311664-302X10.3389/fmicb.2019.00631info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:48Zoai:www.repository.utl.pt:10400.5/22242Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:42.933360Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
title Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
spellingShingle Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
Pinho, MD
Streptococcus canis
multilocus sequence typing
M-like protein (SCM) gene
wildlife
genome
title_short Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
title_full Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
title_fullStr Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
title_full_unstemmed Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
title_sort Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
author Pinho, MD
author_facet Pinho, MD
Foster, G
Pomba, C.
Machado, MP
Baily, JL
Kuiken, T
Melo-Cristino, J
Ramirez, M
Vaz, T
Giao, M
author_role author
author2 Foster, G
Pomba, C.
Machado, MP
Baily, JL
Kuiken, T
Melo-Cristino, J
Ramirez, M
Vaz, T
Giao, M
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pinho, MD
Foster, G
Pomba, C.
Machado, MP
Baily, JL
Kuiken, T
Melo-Cristino, J
Ramirez, M
Vaz, T
Giao, M
dc.subject.por.fl_str_mv Streptococcus canis
multilocus sequence typing
M-like protein (SCM) gene
wildlife
genome
topic Streptococcus canis
multilocus sequence typing
M-like protein (SCM) gene
wildlife
genome
description Research Areas: Microbiology
publishDate 2019
dc.date.none.fl_str_mv 2019-03-29
2019-03-29T00:00:00Z
2021-10-13T23:04:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22242
url http://hdl.handle.net/10400.5/22242
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pinho MD, Foster G, Pomba C, Machado MP, Baily JL, Kuiken T, Melo-Cristino J, Ramirez M et al. 2019. Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM. Frontiers in Microbiology 10:631
1664-302X
10.3389/fmicb.2019.00631
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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