Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/22242 |
Resumo: | Research Areas: Microbiology |
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Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCMStreptococcus canismultilocus sequence typingM-like protein (SCM) genewildlifegenomeResearch Areas: MicrobiologyABSTRACT - Streptococcus canis is an animal pathogen which occasionally causes infections in humans. The S. canis M-like protein (SCM) encoded by the scm gene, is its best characterized virulence factor but previous studies suggested it could be absent in a substantial fraction of isolates. We studied the distribution and variability of the scm gene in 188 S. canis isolates recovered from companion animals (n = 152), wild animal species (n = 20), and humans (n = 14). Multilocus sequence typing, including the first characterization of wildlife isolates, showed that the same lineages are present in all animal hosts, raising the possibility of extensive circulation between species. Whole-genome analysis revealed that emm-like genes found previously in S. canis correspond to divergent scm genes, indicating that what was previously believed to correspond to two genes is in fact the same scm locus. We designed primers allowing for the first time the successful amplification of the scm gene in all isolates. Analysis of the scm sequences identified 12 distinct types, which could be divided into two clusters: group I (76%, n = 142) and group II (24%, n = 46) sharing little sequence similarity. The predicted group I SCM showed extensive similarity with each other outside of the N-terminal hypervariable region and a conserved IgG binding domain. This domain was absent from group II SCM variants found in isolates previously thought to lack the scm gene, which also showed greater amino acid variability. Further studies are necessary to elucidate the possible host interacting partners of the group II SCM variants and their role in virulence.Frontiers MediaRepositório da Universidade de LisboaPinho, MDFoster, GPomba, C.Machado, MPBaily, JLKuiken, TMelo-Cristino, JRamirez, MVaz, TGiao, M2021-10-13T23:04:47Z2019-03-292019-03-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22242engPinho MD, Foster G, Pomba C, Machado MP, Baily JL, Kuiken T, Melo-Cristino J, Ramirez M et al. 2019. Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM. Frontiers in Microbiology 10:6311664-302X10.3389/fmicb.2019.00631info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:48Zoai:www.repository.utl.pt:10400.5/22242Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:42.933360Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM |
title |
Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM |
spellingShingle |
Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM Pinho, MD Streptococcus canis multilocus sequence typing M-like protein (SCM) gene wildlife genome |
title_short |
Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM |
title_full |
Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM |
title_fullStr |
Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM |
title_full_unstemmed |
Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM |
title_sort |
Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM |
author |
Pinho, MD |
author_facet |
Pinho, MD Foster, G Pomba, C. Machado, MP Baily, JL Kuiken, T Melo-Cristino, J Ramirez, M Vaz, T Giao, M |
author_role |
author |
author2 |
Foster, G Pomba, C. Machado, MP Baily, JL Kuiken, T Melo-Cristino, J Ramirez, M Vaz, T Giao, M |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Pinho, MD Foster, G Pomba, C. Machado, MP Baily, JL Kuiken, T Melo-Cristino, J Ramirez, M Vaz, T Giao, M |
dc.subject.por.fl_str_mv |
Streptococcus canis multilocus sequence typing M-like protein (SCM) gene wildlife genome |
topic |
Streptococcus canis multilocus sequence typing M-like protein (SCM) gene wildlife genome |
description |
Research Areas: Microbiology |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-03-29 2019-03-29T00:00:00Z 2021-10-13T23:04:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/22242 |
url |
http://hdl.handle.net/10400.5/22242 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pinho MD, Foster G, Pomba C, Machado MP, Baily JL, Kuiken T, Melo-Cristino J, Ramirez M et al. 2019. Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM. Frontiers in Microbiology 10:631 1664-302X 10.3389/fmicb.2019.00631 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131160322768896 |