Immunology and mammary cancer development: addressing the role of mast cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/31169 |
Resumo: | Background: Mammary cancer is one of the most frequent cancers worldwide. Mast cells are among the cells of tumor microenvironment and have been associated with increased angiogenesis and poor prognosis. Despite this, the role of mast cells on mammary cancer is not fully elucidated. In this way, this work studied the role of mast cells in a rat model of mammary cancer chemically-induced. Material and methods: All experiments were performed in accordance with the Portuguese and European legislation on the protection of animals used for scientific purposes. The experiments were approved by the Portuguese (no.008961) and University (CE_12-2013) Ethics Committees. Thirty-four female Sprague-Dawley rats were randomly divided into five experimental groups. At seven weeks of age, mammary tumors’ development was induced in animals from groups I, II, III (n=10+10+10) by a single intraperitoneal injection of the carcinogen N-methyl-N-nitrosourea (MNU). Groups II and IV (n=2) were treated with ketotifen in drinking water (1 mg/kg/day, 7 days/week) immediately after the MNU administration for 18 weeks, while the group III received the ketotifen after the development of the first mammary tumor. Groups I and V (n=2) received only water. Animals were sacrificed at 25 weeks of age by an overdose of ketamine and xylazine, followed by an exsanguination by cardiac puncture. Mammary tumors were collected and immersed in formalin for posterior analysis. Tumors’ vascularization, proliferation and apoptosis were also assessed by immunohistochemistry (Vascular Endothelial Growth Factor (VEGF)-A, Ki-67, and caspases-3 and -9). Results: Animals from groups IV and V did not develop any mammary tumor. Twenty-one animals (six animals from group I, eight animals from group II and seven animals from group III) developed a total of 58 mammary tumors, mainly classified as papillary non-invasive carcinomas. Tumors’ vascularization was similar among groups (p>0.05). Mammary tumors from group II exhibited the lowest proliferation (p<0.05) and apoptotic indexes. Conclusions: The mainly positive effect of the ketotifen administration seems to be the reduction of tumor proliferation when the drug was administered before mammary tumor development. |
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Immunology and mammary cancer development: addressing the role of mast cellsBackground: Mammary cancer is one of the most frequent cancers worldwide. Mast cells are among the cells of tumor microenvironment and have been associated with increased angiogenesis and poor prognosis. Despite this, the role of mast cells on mammary cancer is not fully elucidated. In this way, this work studied the role of mast cells in a rat model of mammary cancer chemically-induced. Material and methods: All experiments were performed in accordance with the Portuguese and European legislation on the protection of animals used for scientific purposes. The experiments were approved by the Portuguese (no.008961) and University (CE_12-2013) Ethics Committees. Thirty-four female Sprague-Dawley rats were randomly divided into five experimental groups. At seven weeks of age, mammary tumors’ development was induced in animals from groups I, II, III (n=10+10+10) by a single intraperitoneal injection of the carcinogen N-methyl-N-nitrosourea (MNU). Groups II and IV (n=2) were treated with ketotifen in drinking water (1 mg/kg/day, 7 days/week) immediately after the MNU administration for 18 weeks, while the group III received the ketotifen after the development of the first mammary tumor. Groups I and V (n=2) received only water. Animals were sacrificed at 25 weeks of age by an overdose of ketamine and xylazine, followed by an exsanguination by cardiac puncture. Mammary tumors were collected and immersed in formalin for posterior analysis. Tumors’ vascularization, proliferation and apoptosis were also assessed by immunohistochemistry (Vascular Endothelial Growth Factor (VEGF)-A, Ki-67, and caspases-3 and -9). Results: Animals from groups IV and V did not develop any mammary tumor. Twenty-one animals (six animals from group I, eight animals from group II and seven animals from group III) developed a total of 58 mammary tumors, mainly classified as papillary non-invasive carcinomas. Tumors’ vascularization was similar among groups (p>0.05). Mammary tumors from group II exhibited the lowest proliferation (p<0.05) and apoptotic indexes. Conclusions: The mainly positive effect of the ketotifen administration seems to be the reduction of tumor proliferation when the drug was administered before mammary tumor development.European Society for Clinical Investigation (ESCI) Virtual Meeting 2020 - COVID edition2022-02-23T15:05:14Z2022-02-232020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/31169http://hdl.handle.net/10174/31169engFaustino-Rocha AI, Oliveira PA, Gama A, Ferreira R, Ginja M. 2020. Immunology and mammary cancer development: addressing the role of mast cells. European Society for Clinical Investigation (ESCI) Virtual Meeting 2020 - COVID edition, 20 a 30 de setembro.simnaonaoanafaustino@uevora.ptndndndnd206Faustino-Rocha, Ana IsabelOliveira, Paula AlexandraGama, AdelinaFerreira, RitaGinja, Márioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:27:38Zoai:dspace.uevora.pt:10174/31169Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:19:34.744040Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Immunology and mammary cancer development: addressing the role of mast cells |
title |
Immunology and mammary cancer development: addressing the role of mast cells |
spellingShingle |
Immunology and mammary cancer development: addressing the role of mast cells Faustino-Rocha, Ana Isabel |
title_short |
Immunology and mammary cancer development: addressing the role of mast cells |
title_full |
Immunology and mammary cancer development: addressing the role of mast cells |
title_fullStr |
Immunology and mammary cancer development: addressing the role of mast cells |
title_full_unstemmed |
Immunology and mammary cancer development: addressing the role of mast cells |
title_sort |
Immunology and mammary cancer development: addressing the role of mast cells |
author |
Faustino-Rocha, Ana Isabel |
author_facet |
Faustino-Rocha, Ana Isabel Oliveira, Paula Alexandra Gama, Adelina Ferreira, Rita Ginja, Mário |
author_role |
author |
author2 |
Oliveira, Paula Alexandra Gama, Adelina Ferreira, Rita Ginja, Mário |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Faustino-Rocha, Ana Isabel Oliveira, Paula Alexandra Gama, Adelina Ferreira, Rita Ginja, Mário |
description |
Background: Mammary cancer is one of the most frequent cancers worldwide. Mast cells are among the cells of tumor microenvironment and have been associated with increased angiogenesis and poor prognosis. Despite this, the role of mast cells on mammary cancer is not fully elucidated. In this way, this work studied the role of mast cells in a rat model of mammary cancer chemically-induced. Material and methods: All experiments were performed in accordance with the Portuguese and European legislation on the protection of animals used for scientific purposes. The experiments were approved by the Portuguese (no.008961) and University (CE_12-2013) Ethics Committees. Thirty-four female Sprague-Dawley rats were randomly divided into five experimental groups. At seven weeks of age, mammary tumors’ development was induced in animals from groups I, II, III (n=10+10+10) by a single intraperitoneal injection of the carcinogen N-methyl-N-nitrosourea (MNU). Groups II and IV (n=2) were treated with ketotifen in drinking water (1 mg/kg/day, 7 days/week) immediately after the MNU administration for 18 weeks, while the group III received the ketotifen after the development of the first mammary tumor. Groups I and V (n=2) received only water. Animals were sacrificed at 25 weeks of age by an overdose of ketamine and xylazine, followed by an exsanguination by cardiac puncture. Mammary tumors were collected and immersed in formalin for posterior analysis. Tumors’ vascularization, proliferation and apoptosis were also assessed by immunohistochemistry (Vascular Endothelial Growth Factor (VEGF)-A, Ki-67, and caspases-3 and -9). Results: Animals from groups IV and V did not develop any mammary tumor. Twenty-one animals (six animals from group I, eight animals from group II and seven animals from group III) developed a total of 58 mammary tumors, mainly classified as papillary non-invasive carcinomas. Tumors’ vascularization was similar among groups (p>0.05). Mammary tumors from group II exhibited the lowest proliferation (p<0.05) and apoptotic indexes. Conclusions: The mainly positive effect of the ketotifen administration seems to be the reduction of tumor proliferation when the drug was administered before mammary tumor development. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01T00:00:00Z 2022-02-23T15:05:14Z 2022-02-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/31169 http://hdl.handle.net/10174/31169 |
url |
http://hdl.handle.net/10174/31169 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Faustino-Rocha AI, Oliveira PA, Gama A, Ferreira R, Ginja M. 2020. Immunology and mammary cancer development: addressing the role of mast cells. European Society for Clinical Investigation (ESCI) Virtual Meeting 2020 - COVID edition, 20 a 30 de setembro. sim nao nao anafaustino@uevora.pt nd nd nd nd 206 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
European Society for Clinical Investigation (ESCI) Virtual Meeting 2020 - COVID edition |
publisher.none.fl_str_mv |
European Society for Clinical Investigation (ESCI) Virtual Meeting 2020 - COVID edition |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136677103403008 |