Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression

Detalhes bibliográficos
Autor(a) principal: Nunes-Cabaço, H.
Data de Publicação: 2010
Outros Autores: C Ribot, Julie, Caramalho, Íris, Serra-Caetano, Ana, Silva-Santos, Bruno, E Sousa, Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/11747
Resumo: © 2010 Australasian Society for Immunology Inc. All rights reserved
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spelling Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expressionFoxp3Human T-cell developmentMurine T-cell developmentRegulatory T cellsThymus© 2010 Australasian Society for Immunology Inc. All rights reservedThe thymus generates a T-cell lineage dedicated to immune regulation, 'naturally occurring' regulatory T cells, best specified by the forkhead family transcription factor Foxp3. Here, we have conducted a parallel study in humans and mice where we have dissected the earliest stages of Foxp3 induction during thymocyte development. By analyzing a large collection of 21 human thymuses we show that Foxp3 can be consistently detected in CD4 immature single positive thymocytes that precede the CD4(+)CD8(+) (double positive, DP) stage. The reduced levels of CD3 expression found at this stage of human thymocyte development raise the question of TCR (T-cell receptor) requirement for Foxp3 induction. We further show that, in mice, Foxp3 expression was also detected in pre-DP thymocytes of TCRalpha-sufficient but not in TCRalpha-deficient animals, genetically showing the TCR dependence of Foxp3 expression at pre-DP stages of T-cell development.This work was supported by grant (PTDC/SAU-MI/66248/2006) from ‘Fundação para a Ciência e a Tecnologia’ (FCT) and by ‘Programa Operacional Ciência e Inovação 2010’ (POCI2010) to AES and by a European Molecular Biology Organization Installation Grant to BSS. HNC received a scholarship from FCT co-financed by POCI 2010, and JCR received a post-doctoral fellowship from FCT.Nature Publishing GroupRepositório da Universidade de LisboaNunes-Cabaço, H.C Ribot, JulieCaramalho, ÍrisSerra-Caetano, AnaSilva-Santos, BrunoE Sousa, Ana2014-08-27T09:52:20Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/11747engImmunology and Cell Biology (2010), 1–60818-9641http://dx.doi.org/10.1038/icb.2010.4metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:58:12Zoai:repositorio.ul.pt:10451/11747Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:35:21.962277Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression
title Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression
spellingShingle Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression
Nunes-Cabaço, H.
Foxp3
Human T-cell development
Murine T-cell development
Regulatory T cells
Thymus
title_short Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression
title_full Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression
title_fullStr Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression
title_full_unstemmed Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression
title_sort Foxp3 induction in human and murine thymus precedes the CD4+ CD8+ stage but requires early T-cell receptor expression
author Nunes-Cabaço, H.
author_facet Nunes-Cabaço, H.
C Ribot, Julie
Caramalho, Íris
Serra-Caetano, Ana
Silva-Santos, Bruno
E Sousa, Ana
author_role author
author2 C Ribot, Julie
Caramalho, Íris
Serra-Caetano, Ana
Silva-Santos, Bruno
E Sousa, Ana
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Nunes-Cabaço, H.
C Ribot, Julie
Caramalho, Íris
Serra-Caetano, Ana
Silva-Santos, Bruno
E Sousa, Ana
dc.subject.por.fl_str_mv Foxp3
Human T-cell development
Murine T-cell development
Regulatory T cells
Thymus
topic Foxp3
Human T-cell development
Murine T-cell development
Regulatory T cells
Thymus
description © 2010 Australasian Society for Immunology Inc. All rights reserved
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2014-08-27T09:52:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/11747
url http://hdl.handle.net/10451/11747
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Immunology and Cell Biology (2010), 1–6
0818-9641
http://dx.doi.org/10.1038/icb.2010.4
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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