Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation

Detalhes bibliográficos
Autor(a) principal: Campos, Elisa
Data de Publicação: 2011
Outros Autores: Moura, Teresa F., Oliva, Abel, Leandro, Paula, Soveral, Graça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/3901
Resumo: NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. May 2011; 408 (3): 477-481.
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spelling Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeationAquaporin 3Glycerol permeabilityWater transportErythrocyteBovineNOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. May 2011; 408 (3): 477-481.In general, erythrocytes are highly permeable to water, urea and glycerol. However, expression of aquaporin isoforms in erythrocytes appears to be species characteristic. In the present study, human (hRBC) and bovine (bRBC) erythrocytes were chosen for comparative studies due to their significant difference in membrane glycerol permeability. Osmotic water permeability (Pf) at 23 ºC was (2.89 ± 0.37) × 10-2 and (5.12 ± 0.61) × 10-2 cm s-1 for human and bovine cells respectively, with similar activation energies for water transport. Glycerol permeability (Pgly) for human ((1.37 ± 0.26) × 10-5 cm s-1) differed in three orders of magnitude from bovine erythrocytes ((5.82 ± 0.37) ×10-8 cm s-1) that also showed higher activation energy for glycerol transport. When compared to human, bovine erythrocytes showed a similar expression pattern of AQP1 glycosylated forms on immunoblot analysis, though in slight higher levels, which could be correlated with the 1.5-fold larger Pf found. However, AQP3 expression was not detectable. Immunofluorescence analysis confirmed the absence of AQP3 expression in bovine erythrocyte membranes. In conclusion, lack of AQP3 in bovine erythrocytes points to the lipid pathway as responsible for glycerol permeation and explains the low glycerol permeability and high Ea for transport observed in ruminants.ElsevierRepositório da Universidade de LisboaCampos, ElisaMoura, Teresa F.Oliva, AbelLeandro, PaulaSoveral, Graça2011-08-10T13:05:44Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/3901engBiochemical and Biophysical Research Communications. May 2011; 408 (3): 477-4810006-291Xhttp://dx.doi.org/10.1016/j.bbrc.2011.04.057info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:44:29Zoai:repositorio.ul.pt:10451/3901Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:29:36.736999Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
title Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
spellingShingle Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
Campos, Elisa
Aquaporin 3
Glycerol permeability
Water transport
Erythrocyte
Bovine
title_short Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
title_full Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
title_fullStr Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
title_full_unstemmed Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
title_sort Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
author Campos, Elisa
author_facet Campos, Elisa
Moura, Teresa F.
Oliva, Abel
Leandro, Paula
Soveral, Graça
author_role author
author2 Moura, Teresa F.
Oliva, Abel
Leandro, Paula
Soveral, Graça
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Campos, Elisa
Moura, Teresa F.
Oliva, Abel
Leandro, Paula
Soveral, Graça
dc.subject.por.fl_str_mv Aquaporin 3
Glycerol permeability
Water transport
Erythrocyte
Bovine
topic Aquaporin 3
Glycerol permeability
Water transport
Erythrocyte
Bovine
description NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. May 2011; 408 (3): 477-481.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-10T13:05:44Z
2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/3901
url http://hdl.handle.net/10451/3901
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochemical and Biophysical Research Communications. May 2011; 408 (3): 477-481
0006-291X
http://dx.doi.org/10.1016/j.bbrc.2011.04.057
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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