Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/3901 |
Resumo: | NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. May 2011; 408 (3): 477-481. |
id |
RCAP_c412b79faddcd25d96837ca47b3eabda |
---|---|
oai_identifier_str |
oai:repositorio.ul.pt:10451/3901 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeationAquaporin 3Glycerol permeabilityWater transportErythrocyteBovineNOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. May 2011; 408 (3): 477-481.In general, erythrocytes are highly permeable to water, urea and glycerol. However, expression of aquaporin isoforms in erythrocytes appears to be species characteristic. In the present study, human (hRBC) and bovine (bRBC) erythrocytes were chosen for comparative studies due to their significant difference in membrane glycerol permeability. Osmotic water permeability (Pf) at 23 ºC was (2.89 ± 0.37) × 10-2 and (5.12 ± 0.61) × 10-2 cm s-1 for human and bovine cells respectively, with similar activation energies for water transport. Glycerol permeability (Pgly) for human ((1.37 ± 0.26) × 10-5 cm s-1) differed in three orders of magnitude from bovine erythrocytes ((5.82 ± 0.37) ×10-8 cm s-1) that also showed higher activation energy for glycerol transport. When compared to human, bovine erythrocytes showed a similar expression pattern of AQP1 glycosylated forms on immunoblot analysis, though in slight higher levels, which could be correlated with the 1.5-fold larger Pf found. However, AQP3 expression was not detectable. Immunofluorescence analysis confirmed the absence of AQP3 expression in bovine erythrocyte membranes. In conclusion, lack of AQP3 in bovine erythrocytes points to the lipid pathway as responsible for glycerol permeation and explains the low glycerol permeability and high Ea for transport observed in ruminants.ElsevierRepositório da Universidade de LisboaCampos, ElisaMoura, Teresa F.Oliva, AbelLeandro, PaulaSoveral, Graça2011-08-10T13:05:44Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/3901engBiochemical and Biophysical Research Communications. May 2011; 408 (3): 477-4810006-291Xhttp://dx.doi.org/10.1016/j.bbrc.2011.04.057info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:44:29Zoai:repositorio.ul.pt:10451/3901Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:29:36.736999Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation |
title |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation |
spellingShingle |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation Campos, Elisa Aquaporin 3 Glycerol permeability Water transport Erythrocyte Bovine |
title_short |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation |
title_full |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation |
title_fullStr |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation |
title_full_unstemmed |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation |
title_sort |
Lack of Aquaporin 3 in bovine erythrocyte membranes correlates with low glycerol permeation |
author |
Campos, Elisa |
author_facet |
Campos, Elisa Moura, Teresa F. Oliva, Abel Leandro, Paula Soveral, Graça |
author_role |
author |
author2 |
Moura, Teresa F. Oliva, Abel Leandro, Paula Soveral, Graça |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Campos, Elisa Moura, Teresa F. Oliva, Abel Leandro, Paula Soveral, Graça |
dc.subject.por.fl_str_mv |
Aquaporin 3 Glycerol permeability Water transport Erythrocyte Bovine |
topic |
Aquaporin 3 Glycerol permeability Water transport Erythrocyte Bovine |
description |
NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. May 2011; 408 (3): 477-481. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-10T13:05:44Z 2011 2011-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/3901 |
url |
http://hdl.handle.net/10451/3901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemical and Biophysical Research Communications. May 2011; 408 (3): 477-481 0006-291X http://dx.doi.org/10.1016/j.bbrc.2011.04.057 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134181047926784 |