Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/33755 |
Resumo: | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol25-hydroxycholesterolFusionMeasles virusPeptide© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Measles virus (MV) infection is re-emerging, despite the availability of an effective vaccine. The mechanism of MV entry into a target cell relies on coordinated action between the MV hemagglutinin (H) receptor binding protein and the fusion envelope glycoprotein (F) which mediates fusion between the viral and cell membranes. Peptides derived from the C-terminal heptad repeat (HRC) of F can interfere with this process, blocking MV infection. As previously described, biophysical properties of HRC-derived peptides modulate their antiviral potency. In this work, we characterized a MV peptide fusion inhibitor conjugated to 25-hydroxycholesterol (25HC), a cholesterol derivative with intrinsic antiviral activity, and evaluated its interaction with membrane model systems and human blood cells. The peptide (MV–HC) has a 90% inhibitory concentration (IC90) several logs more advantageous than the equivalent peptide bearing a polyethylene glycol (PEG)-cholesterol moiety. In membrane interaction studies, MV–HC shows a preference for pure 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) monolayers and membranes rich in sphingomyelin, and interacts less with POPC:cholesterol membranes. MV–HC tends to self-aggregate in aqueous solution, in a concentration-dependent manner. Our results suggest that increased membrane interaction dynamicity results from 25HC conjugation, with a concomitant increase in peptide antiviral efficacy.This work was supported by Fundação para a Ciência e Tecnologia—Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) grant PTDC/BBB-BQB/3494/2014, and NIH AI119762, AI121349, and NS091263 to M.P. B.G. also acknowledges FCT-MCTES fellowship SPRH/BD/52431/2013.MDPIRepositório da Universidade de LisboaGomes, BárbaraSantos, Nuno C.Porotto, Matteo2018-06-05T14:05:03Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/33755engMolecules 2017, 22, 18691420-304910.3390/molecules22111869info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:28:45Zoai:repositorio.ul.pt:10451/33755Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:48:40.608055Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol |
title |
Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol |
spellingShingle |
Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol Gomes, Bárbara 25-hydroxycholesterol Fusion Measles virus Peptide |
title_short |
Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol |
title_full |
Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol |
title_fullStr |
Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol |
title_full_unstemmed |
Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol |
title_sort |
Biophysical properties and antiviral activities of measles fusion protein derived peptide conjugated with 25-Hydroxycholesterol |
author |
Gomes, Bárbara |
author_facet |
Gomes, Bárbara Santos, Nuno C. Porotto, Matteo |
author_role |
author |
author2 |
Santos, Nuno C. Porotto, Matteo |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Gomes, Bárbara Santos, Nuno C. Porotto, Matteo |
dc.subject.por.fl_str_mv |
25-hydroxycholesterol Fusion Measles virus Peptide |
topic |
25-hydroxycholesterol Fusion Measles virus Peptide |
description |
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z 2018-06-05T14:05:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/33755 |
url |
http://hdl.handle.net/10451/33755 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules 2017, 22, 1869 1420-3049 10.3390/molecules22111869 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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