Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/84873 |
Resumo: | Mussels secrete protein-based byssal threads to tether to rocks, ships, and other organisms underwater. The secreted marine mussel adhesive proteins (MAPs) contain the peculiar amino acid L-3,4-dihydroxyphenylalanine (DOPA), whose catechol group content contributes greatly to their outstanding adhesive properties. Inspired by such mussel bioadhesion, we demonstrate that catechol-modified polysaccharides can be used to obtain adhesive membranes using the compaction of polyelectrolyte complexes (CoPEC) method. It is a simple and versatile approach that uses polyelectrolyte complexes as building blocks that coalesce and dry as membrane constructs simply as a result of sedimentation and mild temperature. We used two natural and biocompatible polymers: chitosan (CHI) as a polycation and hyaluronic acid (HA) as a polyanion. The CoPEC technique also allowed the entrapment of ternary bioactive glass nanoparticles to stimulate mineralization. Moreover, combinations of these polymers modified with catechol groups were made to enhance the adhesive properties of the assembled membranes. Extensive physico-chemical characterization was performed to investigate the successful production of composite CoPEC membranes in terms of surface morphology, wettability, stability, mechanical performance, in vitro bioactivity, and cellular behavior. Considering the promising properties exhibited by the obtained membranes, new adhesives suitable for the regeneration of hard tissues can be envisaged. |
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Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regenerationPolyelectrolyte complexationChitosanHyaluronic acidCatecholBioactive glass nanoparticlesScience & TechnologyMussels secrete protein-based byssal threads to tether to rocks, ships, and other organisms underwater. The secreted marine mussel adhesive proteins (MAPs) contain the peculiar amino acid L-3,4-dihydroxyphenylalanine (DOPA), whose catechol group content contributes greatly to their outstanding adhesive properties. Inspired by such mussel bioadhesion, we demonstrate that catechol-modified polysaccharides can be used to obtain adhesive membranes using the compaction of polyelectrolyte complexes (CoPEC) method. It is a simple and versatile approach that uses polyelectrolyte complexes as building blocks that coalesce and dry as membrane constructs simply as a result of sedimentation and mild temperature. We used two natural and biocompatible polymers: chitosan (CHI) as a polycation and hyaluronic acid (HA) as a polyanion. The CoPEC technique also allowed the entrapment of ternary bioactive glass nanoparticles to stimulate mineralization. Moreover, combinations of these polymers modified with catechol groups were made to enhance the adhesive properties of the assembled membranes. Extensive physico-chemical characterization was performed to investigate the successful production of composite CoPEC membranes in terms of surface morphology, wettability, stability, mechanical performance, in vitro bioactivity, and cellular behavior. Considering the promising properties exhibited by the obtained membranes, new adhesives suitable for the regeneration of hard tissues can be envisaged.This research was funded by the Portuguese Foundation for Science and Technology (FCT, project numbers PTDC/NAN-MAT/31036/2017 and PTDC/BTM-MAT/28123/2017) and European program FEDER/FEEI. RRC acknowledges FCT for support through grant CEECIND/02842/2017.Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoFonseca, Mário C.Vale, A. CatarinaCosta, Rui R.Reis, R. L.Alves, N. M.20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/84873engFonseca, M.C.; Vale, A.C.; Costa, R.R.; Reis, R.L.; Alves, N.M. Exploiting Polyelectrolyte Complexation for the Development of Adhesive and Bioactive Membranes Envisaging Guided Tissue Regeneration. J. Funct. Biomater. 2023, 14, 3. https://doi.org/10.3390/jfb140100032079-498310.3390/jfb140100033https://www.mdpi.com/2079-4983/14/1/3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-23T01:28:00Zoai:repositorium.sdum.uminho.pt:1822/84873Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:32.900875Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration |
title |
Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration |
spellingShingle |
Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration Fonseca, Mário C. Polyelectrolyte complexation Chitosan Hyaluronic acid Catechol Bioactive glass nanoparticles Science & Technology |
title_short |
Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration |
title_full |
Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration |
title_fullStr |
Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration |
title_full_unstemmed |
Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration |
title_sort |
Exploiting polyelectrolyte complexation for the development of adhesive and bioactive membranes envisaging guided tissue regeneration |
author |
Fonseca, Mário C. |
author_facet |
Fonseca, Mário C. Vale, A. Catarina Costa, Rui R. Reis, R. L. Alves, N. M. |
author_role |
author |
author2 |
Vale, A. Catarina Costa, Rui R. Reis, R. L. Alves, N. M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fonseca, Mário C. Vale, A. Catarina Costa, Rui R. Reis, R. L. Alves, N. M. |
dc.subject.por.fl_str_mv |
Polyelectrolyte complexation Chitosan Hyaluronic acid Catechol Bioactive glass nanoparticles Science & Technology |
topic |
Polyelectrolyte complexation Chitosan Hyaluronic acid Catechol Bioactive glass nanoparticles Science & Technology |
description |
Mussels secrete protein-based byssal threads to tether to rocks, ships, and other organisms underwater. The secreted marine mussel adhesive proteins (MAPs) contain the peculiar amino acid L-3,4-dihydroxyphenylalanine (DOPA), whose catechol group content contributes greatly to their outstanding adhesive properties. Inspired by such mussel bioadhesion, we demonstrate that catechol-modified polysaccharides can be used to obtain adhesive membranes using the compaction of polyelectrolyte complexes (CoPEC) method. It is a simple and versatile approach that uses polyelectrolyte complexes as building blocks that coalesce and dry as membrane constructs simply as a result of sedimentation and mild temperature. We used two natural and biocompatible polymers: chitosan (CHI) as a polycation and hyaluronic acid (HA) as a polyanion. The CoPEC technique also allowed the entrapment of ternary bioactive glass nanoparticles to stimulate mineralization. Moreover, combinations of these polymers modified with catechol groups were made to enhance the adhesive properties of the assembled membranes. Extensive physico-chemical characterization was performed to investigate the successful production of composite CoPEC membranes in terms of surface morphology, wettability, stability, mechanical performance, in vitro bioactivity, and cellular behavior. Considering the promising properties exhibited by the obtained membranes, new adhesives suitable for the regeneration of hard tissues can be envisaged. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/84873 |
url |
https://hdl.handle.net/1822/84873 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fonseca, M.C.; Vale, A.C.; Costa, R.R.; Reis, R.L.; Alves, N.M. Exploiting Polyelectrolyte Complexation for the Development of Adhesive and Bioactive Membranes Envisaging Guided Tissue Regeneration. J. Funct. Biomater. 2023, 14, 3. https://doi.org/10.3390/jfb14010003 2079-4983 10.3390/jfb14010003 3 https://www.mdpi.com/2079-4983/14/1/3 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132288874708992 |