Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Margarida
Data de Publicação: 2015
Outros Autores: Andreu, David, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/23610
Resumo: © 2015 Wiley Periodicals, Inc.
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spelling Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell typesCell penetrating peptidesNrTPUptake kineticsMicroscopy© 2015 Wiley Periodicals, Inc.Nucleolar targeting peptides (NrTPs) are a family of cell penetrating peptides (CPPs) derived from crotamine, a rattlesnake venom toxin. They were named NrTPs for their remarkable nucleolus-homing properties and have been studied for their potential as drug delivery vehicles. Live cell microscopy experiments were conducted to monitor NrTP uptake and distribution in different cell types, including primary cells (PBMCs and erythrocytes) and different immortalized cell lines (HeLa, BHK21, BV-173, and MOLT-4). Uptake dependence on cell type (primary vs. immortalized, suspension vs. adherent, cancer vs. healthy cells), peptide concentration and cell viability were evaluated. To gain further insight on the internalization mechanism, uptake kinetics was also monitored. Results showed the uptake and distribution pattern as strongly dependent on peptide sequence, peptide concentration and membrane constituents. Under similar conditions, NrTP6 is more internalized than NrTP1, NrTP2 and NrTP5. Additionally, while internalization of NrTP7 and NrTP8 may cause cytotoxicity, NrTP6 is noncytotoxic. Higher peptide concentrations can be correlated to nucleolar targeting, although even at low concentrations a residual number of cells reveal positive nucleolar labeling. NrTPs were successfully internalized into all cell types tested except erythrocytes.Fundação para a Ciência e a Tecnologia—Ministério da Educação e Ciência (FCT-MEC, Portugal), including M.R. PhD fellowship. Contract grant number: SFRH/BD/37432/2007. Contract grant sponsor: Spanish Ministry of Economy and Competitiveness (MINECO). Contract grant number: SAF2011–24899. Contract grant sponsor: Generalitat de Catalunya. Contract grant number: 2009 SGR 492. Contract grant sponsor: FP7-PEOPLE IRSES project MEMPEPACROSS (European Union)WileyRepositório da Universidade de LisboaRodrigues, MargaridaAndreu, DavidSantos, Nuno C.2016-05-05T15:10:18Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23610engPeptide Science Volume 104 / Number 2 / 101-1091097-028210.1002/bip.22610metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:28:27Zoai:repositorio.ul.pt:10451/23610Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:28:27Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types
title Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types
spellingShingle Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types
Rodrigues, Margarida
Cell penetrating peptides
NrTP
Uptake kinetics
Microscopy
title_short Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types
title_full Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types
title_fullStr Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types
title_full_unstemmed Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types
title_sort Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types
author Rodrigues, Margarida
author_facet Rodrigues, Margarida
Andreu, David
Santos, Nuno C.
author_role author
author2 Andreu, David
Santos, Nuno C.
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rodrigues, Margarida
Andreu, David
Santos, Nuno C.
dc.subject.por.fl_str_mv Cell penetrating peptides
NrTP
Uptake kinetics
Microscopy
topic Cell penetrating peptides
NrTP
Uptake kinetics
Microscopy
description © 2015 Wiley Periodicals, Inc.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-05-05T15:10:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/23610
url http://hdl.handle.net/10451/23610
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Peptide Science Volume 104 / Number 2 / 101-109
1097-0282
10.1002/bip.22610
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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