Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Margarida
Data de Publicação: 2013
Outros Autores: de la Torre, Beatriz G., Andreu, David, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10768
Resumo: © 2013 Elsevier B.V. All rights reserved.
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spelling Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytesNucleolar targeting peptideCell penetrating peptideLymphocyteMonocyteUptakeKinetics© 2013 Elsevier B.V. All rights reserved.Background: Nucleolar targeting peptides (NrTPs), resulting from structural minimization of the rattlesnake toxin crotamine, are a novel family of cell-penetrating peptides (CPPs) shown to internalize and deliver cargos into different cell types. Methods: In this study, we address NrTP kinetics of translocation into primary cells. We used flow cytometry to measure the intracellular uptake of rhodamine B-labeled NrTPs in peripheral blood mononucleated cells (PBMCs). Results: The kinetic profiles for each peptide are concentration-independent but significantly different among NrTPs, pointing out for the amino acid sequence importance. Arginine-containing peptides (NrTP7 and Tat48–60, used for comparison) were found to be more toxic than lysine-containing ones, as expected. On the other hand, one same peptide behaves differently in each of the lymphocyte and monocyte cell populations, suggesting differences in entry mechanism that in turn reflect diversity in cell functionality. Uptake results obtained at 4 °C or using chemical endocytosis inhibitors support the importance of non-endocytic mechanisms in the cellular internalization of NrTP1 and NrTP5, while confirming endocytosis as the main mechanism of NrTPs entry. Conclusion: Overall, both direct translocation and endocytosis mechanisms play a role in NrTP entry. Yet, there is predominance of endocytosis-mediated mechanisms. NrTPs (especially NrTP6) are an excellent intracellular delivery tool, with efficient internalization and no toxicity. General significance: This work validates NrTPs as potential therapeutic tools for, e.g., cancer or inhibition of viral replication and establishes a new comparative and quantitative method to test CPP efficiency.This work was funded by Fundação para a Ciência e a Tecnologia — Ministério da Educação e Ciência (FCT-MEC, Portugal), including M.R. PhD fellowship SFRH/BD/37432/2007, Spanish Ministry of Economy and Competitiveness (MINECO, grant SAF2011-24899), Generalitat de Catalunya (2009 SGR492) and FP7-PEOPLE IRSES project MEMPEPACROSS (European Union).ElsevierRepositório da Universidade de LisboaRodrigues, Margaridade la Torre, Beatriz G.Andreu, DavidSantos, Nuno C.2014-03-21T14:40:15Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10768engBiochimica et Biophysica Acta 1830 (2013) 4554–45630006-3002http://dx.doi.org/10.1016/j.bbagen.2013.05.020metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:56:26Zoai:repositorio.ul.pt:10451/10768Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:34:40.198992Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes
title Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes
spellingShingle Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes
Rodrigues, Margarida
Nucleolar targeting peptide
Cell penetrating peptide
Lymphocyte
Monocyte
Uptake
Kinetics
title_short Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes
title_full Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes
title_fullStr Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes
title_full_unstemmed Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes
title_sort Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes
author Rodrigues, Margarida
author_facet Rodrigues, Margarida
de la Torre, Beatriz G.
Andreu, David
Santos, Nuno C.
author_role author
author2 de la Torre, Beatriz G.
Andreu, David
Santos, Nuno C.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rodrigues, Margarida
de la Torre, Beatriz G.
Andreu, David
Santos, Nuno C.
dc.subject.por.fl_str_mv Nucleolar targeting peptide
Cell penetrating peptide
Lymphocyte
Monocyte
Uptake
Kinetics
topic Nucleolar targeting peptide
Cell penetrating peptide
Lymphocyte
Monocyte
Uptake
Kinetics
description © 2013 Elsevier B.V. All rights reserved.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-03-21T14:40:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10768
url http://hdl.handle.net/10451/10768
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta 1830 (2013) 4554–4563
0006-3002
http://dx.doi.org/10.1016/j.bbagen.2013.05.020
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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