Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Detalhes bibliográficos
Autor(a) principal: Fachada, Vasco
Data de Publicação: 2022
Outros Autores: Rahkila, Paavo, Fachada, Nuno, Turpeinen, Tuomas, Kujala, Urho M., Kainulainen, Heikki
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1016/j.acthis.2022.151869
http://hdl.handle.net/10437/12738
Resumo: Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions. Keywords: Lipid droplets, PLIN5, Type II diabetes, Skeletal muscle, Insulin resistance, Fiber type
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spelling Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetesBIOLOGIALÍPIDOSDIABETES TIPO 2MÚSCULOS ESQUELÉTICOSRESISTÊNCIA À INSULINABIOLOGYLIPIDSTYPE 2 DIABETESSKELETAL MUSCLEINSULIN RESISTANCESkeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions. Keywords: Lipid droplets, PLIN5, Type II diabetes, Skeletal muscle, Insulin resistance, Fiber typeElsevier2022-02-25T12:29:06Z2022-02-01T00:00:00Z2022-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1016/j.acthis.2022.151869http://hdl.handle.net/10437/12738engFachada, VascoRahkila, PaavoFachada, NunoTurpeinen, TuomasKujala, Urho M.Kainulainen, Heikkiinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-09T14:03:11Zoai:recil.ensinolusofona.pt:10437/12738Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:11:24.401931Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
title Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
spellingShingle Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
Fachada, Vasco
BIOLOGIA
LÍPIDOS
DIABETES TIPO 2
MÚSCULOS ESQUELÉTICOS
RESISTÊNCIA À INSULINA
BIOLOGY
LIPIDS
TYPE 2 DIABETES
SKELETAL MUSCLE
INSULIN RESISTANCE
title_short Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
title_full Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
title_fullStr Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
title_full_unstemmed Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
title_sort Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
author Fachada, Vasco
author_facet Fachada, Vasco
Rahkila, Paavo
Fachada, Nuno
Turpeinen, Tuomas
Kujala, Urho M.
Kainulainen, Heikki
author_role author
author2 Rahkila, Paavo
Fachada, Nuno
Turpeinen, Tuomas
Kujala, Urho M.
Kainulainen, Heikki
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fachada, Vasco
Rahkila, Paavo
Fachada, Nuno
Turpeinen, Tuomas
Kujala, Urho M.
Kainulainen, Heikki
dc.subject.por.fl_str_mv BIOLOGIA
LÍPIDOS
DIABETES TIPO 2
MÚSCULOS ESQUELÉTICOS
RESISTÊNCIA À INSULINA
BIOLOGY
LIPIDS
TYPE 2 DIABETES
SKELETAL MUSCLE
INSULIN RESISTANCE
topic BIOLOGIA
LÍPIDOS
DIABETES TIPO 2
MÚSCULOS ESQUELÉTICOS
RESISTÊNCIA À INSULINA
BIOLOGY
LIPIDS
TYPE 2 DIABETES
SKELETAL MUSCLE
INSULIN RESISTANCE
description Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions. Keywords: Lipid droplets, PLIN5, Type II diabetes, Skeletal muscle, Insulin resistance, Fiber type
publishDate 2022
dc.date.none.fl_str_mv 2022-02-25T12:29:06Z
2022-02-01T00:00:00Z
2022-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.acthis.2022.151869
http://hdl.handle.net/10437/12738
url https://doi.org/10.1016/j.acthis.2022.151869
http://hdl.handle.net/10437/12738
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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