Ocular injectable formulation assessment for oxidized dextran-based hydrogels
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/14495 https://doi.org/10.1016/j.actbio.2009.02.008 |
Resumo: | Initiator-free injectable hydrogels are very interesting for drug and/or cell delivery applications, since they can be administered in a minimally invasive way, and avoid the use of potentially harmful chemical initiators. In the current work, oxidized dextran crosslinked with adipic acid dihydrazide hydrogels were further characterized and tuned to produce formulations, with the aim of producing an injectable formulation for the possible treatment of posterior eye diseases. The gelation rate and the hydrogel dissolution profile were shown to be dependent on the balance between the degree of dextran oxidation, and the concentration of both components. For the in vitro studies, rabbit corneal endothelial cells were seeded on the hydrogels to assess cytotoxicity. Hydrogels prepared with low oxidized dextrans were able to promote cell adhesion and proliferation to confluence in just 24 h, while more highly oxidized samples promoted cell adhesion and proliferation, but without achieving confluence. Cell viability studies were performed using MTS assays to verify the non-cytotoxicity of hydrogels and their degradation byproducts, rendering these formulations attractive for further in vivo studies. |
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Ocular injectable formulation assessment for oxidized dextran-based hydrogelsInjectableHydrogelOxidized DextranIn vitroOcular deliveryInitiator-free injectable hydrogels are very interesting for drug and/or cell delivery applications, since they can be administered in a minimally invasive way, and avoid the use of potentially harmful chemical initiators. In the current work, oxidized dextran crosslinked with adipic acid dihydrazide hydrogels were further characterized and tuned to produce formulations, with the aim of producing an injectable formulation for the possible treatment of posterior eye diseases. The gelation rate and the hydrogel dissolution profile were shown to be dependent on the balance between the degree of dextran oxidation, and the concentration of both components. For the in vitro studies, rabbit corneal endothelial cells were seeded on the hydrogels to assess cytotoxicity. Hydrogels prepared with low oxidized dextrans were able to promote cell adhesion and proliferation to confluence in just 24 h, while more highly oxidized samples promoted cell adhesion and proliferation, but without achieving confluence. Cell viability studies were performed using MTS assays to verify the non-cytotoxicity of hydrogels and their degradation byproducts, rendering these formulations attractive for further in vivo studies.Instituto de Investigação Interdisciplinar (financial support of JM – III/BIO/20/2005); Portuguese Foundation for Science and Technology (in the form of fellowship to IJC - SFRH/BPD/19776/2004)Elsevier2009-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/14495http://hdl.handle.net/10316/14495https://doi.org/10.1016/j.actbio.2009.02.008engMAIA, João [et al.] - Ocular injectable formulation assessment for oxidized dextran-based hydrogels. "Acta Biomaterialia". ISSN 1742-7061. 5:6 (2009) 1948-19551742-7061Maia, JoãoRibeiro, Maximiano P.Ventura, CarlaCarvalho, Rui A.Correia, Ilídio J.Gil, Maria H.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-23T08:42:46Zoai:estudogeral.uc.pt:10316/14495Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:59:21.307274Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ocular injectable formulation assessment for oxidized dextran-based hydrogels |
title |
Ocular injectable formulation assessment for oxidized dextran-based hydrogels |
spellingShingle |
Ocular injectable formulation assessment for oxidized dextran-based hydrogels Maia, João Injectable Hydrogel Oxidized Dextran In vitro Ocular delivery |
title_short |
Ocular injectable formulation assessment for oxidized dextran-based hydrogels |
title_full |
Ocular injectable formulation assessment for oxidized dextran-based hydrogels |
title_fullStr |
Ocular injectable formulation assessment for oxidized dextran-based hydrogels |
title_full_unstemmed |
Ocular injectable formulation assessment for oxidized dextran-based hydrogels |
title_sort |
Ocular injectable formulation assessment for oxidized dextran-based hydrogels |
author |
Maia, João |
author_facet |
Maia, João Ribeiro, Maximiano P. Ventura, Carla Carvalho, Rui A. Correia, Ilídio J. Gil, Maria H. |
author_role |
author |
author2 |
Ribeiro, Maximiano P. Ventura, Carla Carvalho, Rui A. Correia, Ilídio J. Gil, Maria H. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Maia, João Ribeiro, Maximiano P. Ventura, Carla Carvalho, Rui A. Correia, Ilídio J. Gil, Maria H. |
dc.subject.por.fl_str_mv |
Injectable Hydrogel Oxidized Dextran In vitro Ocular delivery |
topic |
Injectable Hydrogel Oxidized Dextran In vitro Ocular delivery |
description |
Initiator-free injectable hydrogels are very interesting for drug and/or cell delivery applications, since they can be administered in a minimally invasive way, and avoid the use of potentially harmful chemical initiators. In the current work, oxidized dextran crosslinked with adipic acid dihydrazide hydrogels were further characterized and tuned to produce formulations, with the aim of producing an injectable formulation for the possible treatment of posterior eye diseases. The gelation rate and the hydrogel dissolution profile were shown to be dependent on the balance between the degree of dextran oxidation, and the concentration of both components. For the in vitro studies, rabbit corneal endothelial cells were seeded on the hydrogels to assess cytotoxicity. Hydrogels prepared with low oxidized dextrans were able to promote cell adhesion and proliferation to confluence in just 24 h, while more highly oxidized samples promoted cell adhesion and proliferation, but without achieving confluence. Cell viability studies were performed using MTS assays to verify the non-cytotoxicity of hydrogels and their degradation byproducts, rendering these formulations attractive for further in vivo studies. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/14495 http://hdl.handle.net/10316/14495 https://doi.org/10.1016/j.actbio.2009.02.008 |
url |
http://hdl.handle.net/10316/14495 https://doi.org/10.1016/j.actbio.2009.02.008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
MAIA, João [et al.] - Ocular injectable formulation assessment for oxidized dextran-based hydrogels. "Acta Biomaterialia". ISSN 1742-7061. 5:6 (2009) 1948-1955 1742-7061 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133885303357440 |