Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/74920 |
Resumo: | Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth. By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process. Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions. The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering. Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60â 170 µm, and a stiffness ranging from 1 to 2 MPa. Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture. Besides, cellular proliferation was higher on the scaffolds coated with silk. These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering. |
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Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approachBeta-tricalcium phosphateBMP-2Bone regenerationChitosanSilk Fibroinβ-Tricalcium phosphateScience & TechnologyBone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth. By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process. Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions. The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering. Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60â 170 µm, and a stiffness ranging from 1 to 2 MPa. Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture. Besides, cellular proliferation was higher on the scaffolds coated with silk. These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.The authors especially acknowledge financial support from Coordenaç˜ao de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)—PVE/Project 407035/2013-3. This work was also financially sup ported by Portuguese FCT (PD/BD/135247/2017, SFRH/BPD/93697/2013, SFRH/BPD/100760/2014), PhD programme in Advanced Therapies for Health (PATH) (PD/00169/2013), FCT R&D&I projects with references PTDC/BII-BIO/31570/2017, PTDC/CTM-CTM//29813/2017, PTDC/BII-BIO/28870/2017 and R&D&I Structured Projects with reference NORTE-01-0145-FEDER-000021.IOP PublishingUniversidade do MinhoPiaia, LyaSilva, Simone S.Gomes, Joana M.Franco, Albina RibeiroFernandes, Emanuel MoutaLobo, Flávia Cristina MarquesRodrigues, Luísa C.Leonor, I. B.Fredel, Márcio CSalmoria, Gean V.Hotza, DachamirReis, R. L.20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/74920engPiaia, L., Silva, S. S., Gomes, J. M., R Franco, A., Fernandes, E. M., Lobo, F. C. M., … Reis, R. L. (2021, November 17). Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach. Biomedical Materials. IOP Publishing. http://doi.org/10.1088/1748-605x/ac355a1757-899X10.1088/1748-605X/ac355a34785622https://doi.org/10.1088/1748-605X/ac355ainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-02T01:19:29Zoai:repositorium.sdum.uminho.pt:1822/74920Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:01:26.613934Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach |
title |
Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach |
spellingShingle |
Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach Piaia, Lya Beta-tricalcium phosphate BMP-2 Bone regeneration Chitosan Silk Fibroin β-Tricalcium phosphate Science & Technology |
title_short |
Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach |
title_full |
Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach |
title_fullStr |
Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach |
title_full_unstemmed |
Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach |
title_sort |
Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach |
author |
Piaia, Lya |
author_facet |
Piaia, Lya Silva, Simone S. Gomes, Joana M. Franco, Albina Ribeiro Fernandes, Emanuel Mouta Lobo, Flávia Cristina Marques Rodrigues, Luísa C. Leonor, I. B. Fredel, Márcio C Salmoria, Gean V. Hotza, Dachamir Reis, R. L. |
author_role |
author |
author2 |
Silva, Simone S. Gomes, Joana M. Franco, Albina Ribeiro Fernandes, Emanuel Mouta Lobo, Flávia Cristina Marques Rodrigues, Luísa C. Leonor, I. B. Fredel, Márcio C Salmoria, Gean V. Hotza, Dachamir Reis, R. L. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Piaia, Lya Silva, Simone S. Gomes, Joana M. Franco, Albina Ribeiro Fernandes, Emanuel Mouta Lobo, Flávia Cristina Marques Rodrigues, Luísa C. Leonor, I. B. Fredel, Márcio C Salmoria, Gean V. Hotza, Dachamir Reis, R. L. |
dc.subject.por.fl_str_mv |
Beta-tricalcium phosphate BMP-2 Bone regeneration Chitosan Silk Fibroin β-Tricalcium phosphate Science & Technology |
topic |
Beta-tricalcium phosphate BMP-2 Bone regeneration Chitosan Silk Fibroin β-Tricalcium phosphate Science & Technology |
description |
Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth. By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process. Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions. The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering. Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60â 170 µm, and a stiffness ranging from 1 to 2 MPa. Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture. Besides, cellular proliferation was higher on the scaffolds coated with silk. These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/74920 |
url |
https://hdl.handle.net/1822/74920 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Piaia, L., Silva, S. S., Gomes, J. M., R Franco, A., Fernandes, E. M., Lobo, F. C. M., … Reis, R. L. (2021, November 17). Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach. Biomedical Materials. IOP Publishing. http://doi.org/10.1088/1748-605x/ac355a 1757-899X 10.1088/1748-605X/ac355a 34785622 https://doi.org/10.1088/1748-605X/ac355a |
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info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IOP Publishing |
publisher.none.fl_str_mv |
IOP Publishing |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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