Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/32110 |
Resumo: | The impact of metals (Cd, Cr, Cu and Zn) on growth, cell volume and cell division of the freshwater alga Pseudokirchneriella subcapitata exposed over a period of 72 h was investigated. The algal cells were exposed to three nominal concentrations of each metal: low (closed to 72 h-EC10 values), intermediate (closed to 72 h-EC50 values) and high (upper than 72 h-EC90 values). The exposure to low metal concentrations resulted in a decrease of cell volume. On the contrary, for the highest metal concentrations an increase of cell volume was observed; this effect was particularly notorious for Cd and less pronounced for Zn. Two behaviours were found when algal cells were exposed to intermediate concentrations of metals: Cu(II) and Cr(VI) induced a reduction of cell volume, while Cd(II) and Zn(II) provoked an opposite effect. The simultaneous nucleus staining and cell image analysis, allowed distinguishing three phases in P. subcapitata cell cycle: growth of mother cell; cell division, which includes two divisions of the nucleus; and, release of four autospores. The exposure of P. subcapitata cells to the highest metal concentrations resulted in the arrest of cell growth before the first nucleus division [for Cr(VI) and Cu(II)] or after the second nucleus division but before the cytokinesis (release of autospores) when exposed to Cd(II). The different impact of metals on algal cell volume and cell-cycle progression, suggests that different toxicity mechanisms underlie the action of different metals studied. The simultaneous nucleus staining and cell image analysis, used in the present work, can be a useful tool in the analysis of the toxicity of the pollutants, in P. subcapitata, and help in the elucidation of their different modes of action. |
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Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stressAlgal growthBiovolumeCell divisionCell proliferationCell sizeMetal toxicityScience & TechnologyThe impact of metals (Cd, Cr, Cu and Zn) on growth, cell volume and cell division of the freshwater alga Pseudokirchneriella subcapitata exposed over a period of 72 h was investigated. The algal cells were exposed to three nominal concentrations of each metal: low (closed to 72 h-EC10 values), intermediate (closed to 72 h-EC50 values) and high (upper than 72 h-EC90 values). The exposure to low metal concentrations resulted in a decrease of cell volume. On the contrary, for the highest metal concentrations an increase of cell volume was observed; this effect was particularly notorious for Cd and less pronounced for Zn. Two behaviours were found when algal cells were exposed to intermediate concentrations of metals: Cu(II) and Cr(VI) induced a reduction of cell volume, while Cd(II) and Zn(II) provoked an opposite effect. The simultaneous nucleus staining and cell image analysis, allowed distinguishing three phases in P. subcapitata cell cycle: growth of mother cell; cell division, which includes two divisions of the nucleus; and, release of four autospores. The exposure of P. subcapitata cells to the highest metal concentrations resulted in the arrest of cell growth before the first nucleus division [for Cr(VI) and Cu(II)] or after the second nucleus division but before the cytokinesis (release of autospores) when exposed to Cd(II). The different impact of metals on algal cell volume and cell-cycle progression, suggests that different toxicity mechanisms underlie the action of different metals studied. The simultaneous nucleus staining and cell image analysis, used in the present work, can be a useful tool in the analysis of the toxicity of the pollutants, in P. subcapitata, and help in the elucidation of their different modes of action.The authors thank the Fundacao para a Ciencia e a Tecnologia (FCT) through the Portuguese Government for their financial support of this work through the strategic project-LA23/2013-2014 to IBB. Manuela D. Machado gratefully acknowledges the post-doctoral grant from FCT (SFRH/BPD/72816/2010).Elsevier B.V.Universidade do MinhoMachado, Manuela D.Soares, Eduardo V.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32110engMachado, Manuela D.; Soares, Eduardo V., Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress. Aquatic Toxicology, 147, 1-6, 20141879-15140166-445X10.1016/j.aquatox.2013.11.01724342441www.elsevier.com/locate/aquatoxinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:31Zoai:repositorium.sdum.uminho.pt:1822/32110Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:45.718062Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress |
title |
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress |
spellingShingle |
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress Machado, Manuela D. Algal growth Biovolume Cell division Cell proliferation Cell size Metal toxicity Science & Technology |
title_short |
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress |
title_full |
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress |
title_fullStr |
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress |
title_full_unstemmed |
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress |
title_sort |
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress |
author |
Machado, Manuela D. |
author_facet |
Machado, Manuela D. Soares, Eduardo V. |
author_role |
author |
author2 |
Soares, Eduardo V. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Machado, Manuela D. Soares, Eduardo V. |
dc.subject.por.fl_str_mv |
Algal growth Biovolume Cell division Cell proliferation Cell size Metal toxicity Science & Technology |
topic |
Algal growth Biovolume Cell division Cell proliferation Cell size Metal toxicity Science & Technology |
description |
The impact of metals (Cd, Cr, Cu and Zn) on growth, cell volume and cell division of the freshwater alga Pseudokirchneriella subcapitata exposed over a period of 72 h was investigated. The algal cells were exposed to three nominal concentrations of each metal: low (closed to 72 h-EC10 values), intermediate (closed to 72 h-EC50 values) and high (upper than 72 h-EC90 values). The exposure to low metal concentrations resulted in a decrease of cell volume. On the contrary, for the highest metal concentrations an increase of cell volume was observed; this effect was particularly notorious for Cd and less pronounced for Zn. Two behaviours were found when algal cells were exposed to intermediate concentrations of metals: Cu(II) and Cr(VI) induced a reduction of cell volume, while Cd(II) and Zn(II) provoked an opposite effect. The simultaneous nucleus staining and cell image analysis, allowed distinguishing three phases in P. subcapitata cell cycle: growth of mother cell; cell division, which includes two divisions of the nucleus; and, release of four autospores. The exposure of P. subcapitata cells to the highest metal concentrations resulted in the arrest of cell growth before the first nucleus division [for Cr(VI) and Cu(II)] or after the second nucleus division but before the cytokinesis (release of autospores) when exposed to Cd(II). The different impact of metals on algal cell volume and cell-cycle progression, suggests that different toxicity mechanisms underlie the action of different metals studied. The simultaneous nucleus staining and cell image analysis, used in the present work, can be a useful tool in the analysis of the toxicity of the pollutants, in P. subcapitata, and help in the elucidation of their different modes of action. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/32110 |
url |
http://hdl.handle.net/1822/32110 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Machado, Manuela D.; Soares, Eduardo V., Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress. Aquatic Toxicology, 147, 1-6, 2014 1879-1514 0166-445X 10.1016/j.aquatox.2013.11.017 24342441 www.elsevier.com/locate/aquatox |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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