Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles

Detalhes bibliográficos
Autor(a) principal: Alves-Barroco, Cinthia
Data de Publicação: 2022
Outros Autores: Rivas-García, Lorenzo, Fernandes, Alexandra R., Baptista, Pedro Viana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143487
Resumo: This work was financed by national funds from FCT-Funda??o para a Ci?ncia e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB. FCT?MEC have also acknowledged for SFRH/BD/118350/2016 to CA-B, and Inn-Indigo 00002/2015 RA Detect to LR-G. Publisher Copyright: Copyright © 2022 Alves-Barroco, Rivas-García, Fernandes and Baptista.
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spelling Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy NanoparticlesStreptococcusbiofilm inhibitionbiofilmseradication mature biofilmsgold-silver alloy nanoparticlesMicrobiologyMicrobiology (medical)This work was financed by national funds from FCT-Funda??o para a Ci?ncia e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB. FCT?MEC have also acknowledged for SFRH/BD/118350/2016 to CA-B, and Inn-Indigo 00002/2015 RA Detect to LR-G. Publisher Copyright: Copyright © 2022 Alves-Barroco, Rivas-García, Fernandes and Baptista.Bacterial biofilm is a tri-dimensional complex community of cells at different metabolic stages involved in a matrix of self-produced extracellular polymeric substances. Biofilm formation is part of a defense mechanism that allows the bacteria to survive in hostile environments, such as increasing resistance or tolerance to antimicrobial agents, causing persistent infections hard to treat and impair disease eradication. One such example is bovine mastitis associated with Streptococcus dysgalactiae subsp. dysgalactiae (SDSD), whose worldwide health and economic impact is on the surge. As such, non-conventional nanobased approaches have been proposed as an alternative to tackle biofilm formation and to which pathogenic bacteria fail to adapt. Among these, metallic nanoparticles have gained significant attention, particularly gold and silver nanoparticles, due to their ease of synthesis and impact against microorganism growth. This study provides a proof-of-concept investigation into the use of gold-silver alloy nanoparticles (AuAgNPs) toward eradication of bacterial biofilms. Upon visible light irradiation of AuAgNPs there was considerable disturbance of the biofilms' matrix. The hindering of structural integrity of the biofilm matrix resulted in an increased permeability for entry of antibiotics, which then cause the eradication of biofilm and inhibit subsequent biofilm formation. Additionally, our results that AuAgNPs inhibited the formation of SDSD biofilms via distinct stress pathways that lead to the downregulation of two genes critical for biofilm production, namely, brpA-like encoding biofilm regulatory protein and fbpA fibronectin-binding protein A. This study provides useful information to assist the development of nanoparticle-based strategies for the active treatment of biofilm-related infections triggered by photoirradiation in the visible.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNAlves-Barroco, CinthiaRivas-García, LorenzoFernandes, Alexandra R.Baptista, Pedro Viana2022-09-05T22:19:21Z2022-02-282022-02-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/143487eng1664-302XPURE: 42529736https://doi.org/10.3389/fmicb.2022.841124info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:21:50Zoai:run.unl.pt:10362/143487Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:57.612629Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles
title Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles
spellingShingle Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles
Alves-Barroco, Cinthia
Streptococcus
biofilm inhibition
biofilms
eradication mature biofilms
gold-silver alloy nanoparticles
Microbiology
Microbiology (medical)
title_short Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles
title_full Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles
title_fullStr Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles
title_full_unstemmed Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles
title_sort Light Triggered Enhancement of Antibiotic Efficacy in Biofilm Elimination Mediated by Gold-Silver Alloy Nanoparticles
author Alves-Barroco, Cinthia
author_facet Alves-Barroco, Cinthia
Rivas-García, Lorenzo
Fernandes, Alexandra R.
Baptista, Pedro Viana
author_role author
author2 Rivas-García, Lorenzo
Fernandes, Alexandra R.
Baptista, Pedro Viana
author2_role author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Alves-Barroco, Cinthia
Rivas-García, Lorenzo
Fernandes, Alexandra R.
Baptista, Pedro Viana
dc.subject.por.fl_str_mv Streptococcus
biofilm inhibition
biofilms
eradication mature biofilms
gold-silver alloy nanoparticles
Microbiology
Microbiology (medical)
topic Streptococcus
biofilm inhibition
biofilms
eradication mature biofilms
gold-silver alloy nanoparticles
Microbiology
Microbiology (medical)
description This work was financed by national funds from FCT-Funda??o para a Ci?ncia e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB. FCT?MEC have also acknowledged for SFRH/BD/118350/2016 to CA-B, and Inn-Indigo 00002/2015 RA Detect to LR-G. Publisher Copyright: Copyright © 2022 Alves-Barroco, Rivas-García, Fernandes and Baptista.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-05T22:19:21Z
2022-02-28
2022-02-28T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143487
url http://hdl.handle.net/10362/143487
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-302X
PURE: 42529736
https://doi.org/10.3389/fmicb.2022.841124
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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