LytA-based identification methods can misidentify Streptococcus pneumoniae
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1016/j.diagmicrobio.2016.03.018 |
Resumo: | During surveillance studies we detected, among over 1500 presumptive pneumococci, 11 isolates displaying conflicting or novel results when characterized by widely accepted phenotypic (optochin susceptibility and bile solubility) and genotypic (lytA-BsaAI-RFLP and MLST) identification methods. We aimed to determine the genetic basis for the unexpected results given by lytA-BsaAI-RFLP and investigate the accuracy of the WHO recommended lytA real-time PCR assay to classify these 11 isolates. Three novel lytA-BsaAI-RFLP signatures were found (one in pneumococcus and two in S. mitis). In addition, one pneumococcus displayed the atypical lytA-BsaAI-RFLP signature characteristic of non-pneumococci and two S. pseudopneumoniae displayed the typical lytA-BsaAI-RFLP pattern characteristic of pneumococci. lytA real-time PCR misidentified these three isolates. In conclusion, identification of pneumococci by lytA real-time PCR, and other lytA-based methodologies, may lead to false results. This is of particular relevance in the increasingly frequent colonization studies relying solely on culture-independent methods. |
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LytA-based identification methods can misidentify Streptococcus pneumoniaeIdentificationLytAMolecular methodsReal-time PCRS. pneumoniaeS. pseudopneumoniaeMicrobiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingDuring surveillance studies we detected, among over 1500 presumptive pneumococci, 11 isolates displaying conflicting or novel results when characterized by widely accepted phenotypic (optochin susceptibility and bile solubility) and genotypic (lytA-BsaAI-RFLP and MLST) identification methods. We aimed to determine the genetic basis for the unexpected results given by lytA-BsaAI-RFLP and investigate the accuracy of the WHO recommended lytA real-time PCR assay to classify these 11 isolates. Three novel lytA-BsaAI-RFLP signatures were found (one in pneumococcus and two in S. mitis). In addition, one pneumococcus displayed the atypical lytA-BsaAI-RFLP signature characteristic of non-pneumococci and two S. pseudopneumoniae displayed the typical lytA-BsaAI-RFLP pattern characteristic of pneumococci. lytA real-time PCR misidentified these three isolates. In conclusion, identification of pneumococci by lytA real-time PCR, and other lytA-based methodologies, may lead to false results. This is of particular relevance in the increasingly frequent colonization studies relying solely on culture-independent methods.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)Instituto de Higiene e Medicina Tropical (IHMT)Population health, policies and services (PPS)Global Health and Tropical Medicine (GHTM)RUNSimões, Alexandra S.Tavares, Débora A.Rolo, DoraArdanuy, CarmenGoossens, HermanHenriques-Normark, BirgittaLinares, Josefinade Lencastre, HermíniaSá-Leão, Raquel2018-05-22T22:03:32Z2016-06-012016-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttps://doi.org/10.1016/j.diagmicrobio.2016.03.018eng0732-8893PURE: 2455793http://www.scopus.com/inward/record.url?scp=84963955118&partnerID=8YFLogxKhttps://doi.org/10.1016/j.diagmicrobio.2016.03.018info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:59Zoai:run.unl.pt:10362/37653Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:55.304964Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
LytA-based identification methods can misidentify Streptococcus pneumoniae |
title |
LytA-based identification methods can misidentify Streptococcus pneumoniae |
spellingShingle |
LytA-based identification methods can misidentify Streptococcus pneumoniae Simões, Alexandra S. Identification LytA Molecular methods Real-time PCR S. pneumoniae S. pseudopneumoniae Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
title_short |
LytA-based identification methods can misidentify Streptococcus pneumoniae |
title_full |
LytA-based identification methods can misidentify Streptococcus pneumoniae |
title_fullStr |
LytA-based identification methods can misidentify Streptococcus pneumoniae |
title_full_unstemmed |
LytA-based identification methods can misidentify Streptococcus pneumoniae |
title_sort |
LytA-based identification methods can misidentify Streptococcus pneumoniae |
author |
Simões, Alexandra S. |
author_facet |
Simões, Alexandra S. Tavares, Débora A. Rolo, Dora Ardanuy, Carmen Goossens, Herman Henriques-Normark, Birgitta Linares, Josefina de Lencastre, Hermínia Sá-Leão, Raquel |
author_role |
author |
author2 |
Tavares, Débora A. Rolo, Dora Ardanuy, Carmen Goossens, Herman Henriques-Normark, Birgitta Linares, Josefina de Lencastre, Hermínia Sá-Leão, Raquel |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Instituto de Higiene e Medicina Tropical (IHMT) Population health, policies and services (PPS) Global Health and Tropical Medicine (GHTM) RUN |
dc.contributor.author.fl_str_mv |
Simões, Alexandra S. Tavares, Débora A. Rolo, Dora Ardanuy, Carmen Goossens, Herman Henriques-Normark, Birgitta Linares, Josefina de Lencastre, Hermínia Sá-Leão, Raquel |
dc.subject.por.fl_str_mv |
Identification LytA Molecular methods Real-time PCR S. pneumoniae S. pseudopneumoniae Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
topic |
Identification LytA Molecular methods Real-time PCR S. pneumoniae S. pseudopneumoniae Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
description |
During surveillance studies we detected, among over 1500 presumptive pneumococci, 11 isolates displaying conflicting or novel results when characterized by widely accepted phenotypic (optochin susceptibility and bile solubility) and genotypic (lytA-BsaAI-RFLP and MLST) identification methods. We aimed to determine the genetic basis for the unexpected results given by lytA-BsaAI-RFLP and investigate the accuracy of the WHO recommended lytA real-time PCR assay to classify these 11 isolates. Three novel lytA-BsaAI-RFLP signatures were found (one in pneumococcus and two in S. mitis). In addition, one pneumococcus displayed the atypical lytA-BsaAI-RFLP signature characteristic of non-pneumococci and two S. pseudopneumoniae displayed the typical lytA-BsaAI-RFLP pattern characteristic of pneumococci. lytA real-time PCR misidentified these three isolates. In conclusion, identification of pneumococci by lytA real-time PCR, and other lytA-based methodologies, may lead to false results. This is of particular relevance in the increasingly frequent colonization studies relying solely on culture-independent methods. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 2016-06-01T00:00:00Z 2018-05-22T22:03:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.diagmicrobio.2016.03.018 |
url |
https://doi.org/10.1016/j.diagmicrobio.2016.03.018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0732-8893 PURE: 2455793 http://www.scopus.com/inward/record.url?scp=84963955118&partnerID=8YFLogxK https://doi.org/10.1016/j.diagmicrobio.2016.03.018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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