Streptococcus pneumoniae carriage studies in adults

Detalhes bibliográficos
Autor(a) principal: Miellet, Willem R.
Data de Publicação: 2023
Outros Autores: Almeida, Sónia T., Trzciński, Krzysztof, Sá-Leão, Raquel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158520
Resumo: Funding Information: The authors declare that this study received funding from GlaxoSmithKline Biologicals SA. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication. Funding for this study was provided in part (SA and RS-L) by LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020 – Programa Operacional Competitividade e Internacionalização), the authors received no other form of compensation or financial support related to the development of the manuscript. Funding Information: KT reports receiving consultation and speaking fees and funds for unrestricted research grants from Pfizer, and funds for unrestricted research grants and consultation fees from Merck Sharp & Dohme, all paid directly to his home institution and none related to the present work. RS-L reports receiving consultation and speaking fees from Pfizer and consulting fees from Merck (US). RS-L reports also receiving funds for unrestricted research grants from Pfizer and Merck Sharp & Dohme not related to the present work, paid directly to her home institution. Publisher Copyright: Copyright © 2023 Miellet, Almeida, Trzciński and Sá-Leão.
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spelling Streptococcus pneumoniae carriage studies in adultsImportance, challenges, and key issues to consider when using quantitative PCR-based approachesadultcolonizationnasopharynxoropharynxqPCRsalivaserotypeStreptococcus pneumoniaeMicrobiologyMicrobiology (medical)SDG 3 - Good Health and Well-beingFunding Information: The authors declare that this study received funding from GlaxoSmithKline Biologicals SA. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication. Funding for this study was provided in part (SA and RS-L) by LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020 – Programa Operacional Competitividade e Internacionalização), the authors received no other form of compensation or financial support related to the development of the manuscript. Funding Information: KT reports receiving consultation and speaking fees and funds for unrestricted research grants from Pfizer, and funds for unrestricted research grants and consultation fees from Merck Sharp & Dohme, all paid directly to his home institution and none related to the present work. RS-L reports receiving consultation and speaking fees from Pfizer and consulting fees from Merck (US). RS-L reports also receiving funds for unrestricted research grants from Pfizer and Merck Sharp & Dohme not related to the present work, paid directly to her home institution. Publisher Copyright: Copyright © 2023 Miellet, Almeida, Trzciński and Sá-Leão.Streptococcus pneumoniae causes significant morbidity and mortality among older adults. Detection of pneumococcal carriage is an accepted endpoint in pneumococcal conjugate vaccine studies. However, low sensitivity of culture-based approaches and nasopharyngeal samples have hampered adult S. pneumoniae carriage studies in the past. In contrast, detection of adult S. pneumoniae carriers with qPCR-based approaches can achieve high sensitivity and specificity and qPCR-based testing of oral samples improves accuracy of adult carriage detection. In this Viewpoint we outline a strategy for accurate qPCR-based testing. We recommend a dual-target approach for S. pneumoniae qPCR detection as no genetic target is universally present among or solely unique to it. Furthermore, we advise the evaluation of concordance among quantified qPCR targets to improve the accuracy of S. pneumoniae testing and qPCR-based serotyping. We do not recommend omission of qPCR-based oral sample testing as it will likely result in an underestimation of true adult carrier rates.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNMiellet, Willem R.Almeida, Sónia T.Trzciński, KrzysztofSá-Leão, Raquel2023-09-30T22:18:30Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158520eng1664-302XPURE: 72102602https://doi.org/10.3389/fmicb.2023.1122276info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:57Zoai:run.unl.pt:10362/158520Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:11.036783Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Streptococcus pneumoniae carriage studies in adults
Importance, challenges, and key issues to consider when using quantitative PCR-based approaches
title Streptococcus pneumoniae carriage studies in adults
spellingShingle Streptococcus pneumoniae carriage studies in adults
Miellet, Willem R.
adult
colonization
nasopharynx
oropharynx
qPCR
saliva
serotype
Streptococcus pneumoniae
Microbiology
Microbiology (medical)
SDG 3 - Good Health and Well-being
title_short Streptococcus pneumoniae carriage studies in adults
title_full Streptococcus pneumoniae carriage studies in adults
title_fullStr Streptococcus pneumoniae carriage studies in adults
title_full_unstemmed Streptococcus pneumoniae carriage studies in adults
title_sort Streptococcus pneumoniae carriage studies in adults
author Miellet, Willem R.
author_facet Miellet, Willem R.
Almeida, Sónia T.
Trzciński, Krzysztof
Sá-Leão, Raquel
author_role author
author2 Almeida, Sónia T.
Trzciński, Krzysztof
Sá-Leão, Raquel
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Miellet, Willem R.
Almeida, Sónia T.
Trzciński, Krzysztof
Sá-Leão, Raquel
dc.subject.por.fl_str_mv adult
colonization
nasopharynx
oropharynx
qPCR
saliva
serotype
Streptococcus pneumoniae
Microbiology
Microbiology (medical)
SDG 3 - Good Health and Well-being
topic adult
colonization
nasopharynx
oropharynx
qPCR
saliva
serotype
Streptococcus pneumoniae
Microbiology
Microbiology (medical)
SDG 3 - Good Health and Well-being
description Funding Information: The authors declare that this study received funding from GlaxoSmithKline Biologicals SA. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication. Funding for this study was provided in part (SA and RS-L) by LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020 – Programa Operacional Competitividade e Internacionalização), the authors received no other form of compensation or financial support related to the development of the manuscript. Funding Information: KT reports receiving consultation and speaking fees and funds for unrestricted research grants from Pfizer, and funds for unrestricted research grants and consultation fees from Merck Sharp & Dohme, all paid directly to his home institution and none related to the present work. RS-L reports receiving consultation and speaking fees from Pfizer and consulting fees from Merck (US). RS-L reports also receiving funds for unrestricted research grants from Pfizer and Merck Sharp & Dohme not related to the present work, paid directly to her home institution. Publisher Copyright: Copyright © 2023 Miellet, Almeida, Trzciński and Sá-Leão.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:18:30Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158520
url http://hdl.handle.net/10362/158520
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-302X
PURE: 72102602
https://doi.org/10.3389/fmicb.2023.1122276
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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