Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/7799 |
Resumo: | This article proposes a simple "in-situ" pulse respirometric method for the estimation of four important kinetic and stoichiometric parameters. The method is validated in a suspended biomass nitrifying reactor for the determination of (i) maximum oxygen uptake rate (OURexmax), (ii) oxidation yield (fE), (iii) biomass growth yield (fS), and (iv) affinity constant (KS). OURexmax and fE were directly obtained from respirograms. In the presented case study, a minimum substrate pulse of 10 mgNH4+-NL-1 was necessary to determine OURexmax which was 61.15 ± 4.09 mgO2 L-1 h-1 (5 repetitions). A linear correlation (r2 = 0.93) obtained between OURexmax and the biomass concentration in the reactor suggests that biomass concentration can be estimated from respirometric experiments. The substrate oxidation yield, fE, was determined along 60 days of continuous operation with an average error of 5.6%. The biomass growth yield was indirectly estimated from the substrate oxidation yield fE. The average obtained value (0.10 ± 0.04 mgCOD mg-1COD) was in accordance with the fS estimation by the traditional COD mass balance method under steady-state conditions (0.09 ± 0.01). The affinity constant KS was indirectly estimated after fitting the ascending part of the respirogram to a theoretical model. An average value of 0.48 ± 0.08 mgNH4+-NL-1 was obtained, which is in the range of affinity constants reported in the literature for the nitrification process (0.16-2 mgNH4+-NL-1). |
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Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometryRespirometryGrowth yieldIn-situ pulse techniqueKinetic parametersScience & TechnologyThis article proposes a simple "in-situ" pulse respirometric method for the estimation of four important kinetic and stoichiometric parameters. The method is validated in a suspended biomass nitrifying reactor for the determination of (i) maximum oxygen uptake rate (OURexmax), (ii) oxidation yield (fE), (iii) biomass growth yield (fS), and (iv) affinity constant (KS). OURexmax and fE were directly obtained from respirograms. In the presented case study, a minimum substrate pulse of 10 mgNH4+-NL-1 was necessary to determine OURexmax which was 61.15 ± 4.09 mgO2 L-1 h-1 (5 repetitions). A linear correlation (r2 = 0.93) obtained between OURexmax and the biomass concentration in the reactor suggests that biomass concentration can be estimated from respirometric experiments. The substrate oxidation yield, fE, was determined along 60 days of continuous operation with an average error of 5.6%. The biomass growth yield was indirectly estimated from the substrate oxidation yield fE. The average obtained value (0.10 ± 0.04 mgCOD mg-1COD) was in accordance with the fS estimation by the traditional COD mass balance method under steady-state conditions (0.09 ± 0.01). The affinity constant KS was indirectly estimated after fitting the ascending part of the respirogram to a theoretical model. An average value of 0.48 ± 0.08 mgNH4+-NL-1 was obtained, which is in the range of affinity constants reported in the literature for the nitrification process (0.16-2 mgNH4+-NL-1).European Union in the Framework of the Marie Curie ActionsFundação para a Ciência e a Tecnologia (FCT)Consejo Nacional de Ciencia y TecnologíaJohn Wiley and SonsUniversidade do MinhoOrdaz, AlbertoOliveira, Catarina S.Aguilar, RicardoCarrión, ManuelFerreira, Eugénio C.Alves, M. M.Thalasso, Frédéric2008-052008-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/7799eng"Biotechnology and Bioengineering". ISSN 0006-3592. 100:1 (May 2008) 94-102.0006-359210.1002/bit.2172318078297info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:16:18Zoai:repositorium.sdum.uminho.pt:1822/7799Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:08:49.778710Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry |
title |
Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry |
spellingShingle |
Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry Ordaz, Alberto Respirometry Growth yield In-situ pulse technique Kinetic parameters Science & Technology |
title_short |
Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry |
title_full |
Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry |
title_fullStr |
Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry |
title_full_unstemmed |
Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry |
title_sort |
Kinetic and stoichiometric parameters estimation in a nitrifying bubble column through ‘‘in-situ’’ pulse respirometry |
author |
Ordaz, Alberto |
author_facet |
Ordaz, Alberto Oliveira, Catarina S. Aguilar, Ricardo Carrión, Manuel Ferreira, Eugénio C. Alves, M. M. Thalasso, Frédéric |
author_role |
author |
author2 |
Oliveira, Catarina S. Aguilar, Ricardo Carrión, Manuel Ferreira, Eugénio C. Alves, M. M. Thalasso, Frédéric |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ordaz, Alberto Oliveira, Catarina S. Aguilar, Ricardo Carrión, Manuel Ferreira, Eugénio C. Alves, M. M. Thalasso, Frédéric |
dc.subject.por.fl_str_mv |
Respirometry Growth yield In-situ pulse technique Kinetic parameters Science & Technology |
topic |
Respirometry Growth yield In-situ pulse technique Kinetic parameters Science & Technology |
description |
This article proposes a simple "in-situ" pulse respirometric method for the estimation of four important kinetic and stoichiometric parameters. The method is validated in a suspended biomass nitrifying reactor for the determination of (i) maximum oxygen uptake rate (OURexmax), (ii) oxidation yield (fE), (iii) biomass growth yield (fS), and (iv) affinity constant (KS). OURexmax and fE were directly obtained from respirograms. In the presented case study, a minimum substrate pulse of 10 mgNH4+-NL-1 was necessary to determine OURexmax which was 61.15 ± 4.09 mgO2 L-1 h-1 (5 repetitions). A linear correlation (r2 = 0.93) obtained between OURexmax and the biomass concentration in the reactor suggests that biomass concentration can be estimated from respirometric experiments. The substrate oxidation yield, fE, was determined along 60 days of continuous operation with an average error of 5.6%. The biomass growth yield was indirectly estimated from the substrate oxidation yield fE. The average obtained value (0.10 ± 0.04 mgCOD mg-1COD) was in accordance with the fS estimation by the traditional COD mass balance method under steady-state conditions (0.09 ± 0.01). The affinity constant KS was indirectly estimated after fitting the ascending part of the respirogram to a theoretical model. An average value of 0.48 ± 0.08 mgNH4+-NL-1 was obtained, which is in the range of affinity constants reported in the literature for the nitrification process (0.16-2 mgNH4+-NL-1). |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-05 2008-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/7799 |
url |
https://hdl.handle.net/1822/7799 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Biotechnology and Bioengineering". ISSN 0006-3592. 100:1 (May 2008) 94-102. 0006-3592 10.1002/bit.21723 18078297 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132511676137472 |