SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/14546 |
Resumo: | The honeybee (Apis mellifera) has been threatened by multiple factors including pests and pathogens, pesticidesand loss of locally adapted gene complexes due to replacement and introgression. In western Europe, the geneticintegrity of the native A. m. mellifera (M-lineage) is endangered due to trading and intensive queen breeding withcommercial subspecies of eastern European ancestry (C-lineage). Effective conservation actions require reliablemolecular tools to identify pure-bred A. m. mellifera colonies. Microsatellites have been preferred for identificationof A. m. mellifera stocks across conservation centres. However, owing to high throughput, easy transferabilitybetween laboratories and low genotyping error, SNPs promise to become popular. Here, we compared the resolvingpower of a widely utilized microsatellite set to detect structure and introgression with that of different sets that com-bine a variable number of SNPs selected for their information content and genomic proximity to the microsatelliteloci. Contrary to every SNP data set, microsatellites did not discriminate between the two lineages in the PCA space.Mean introgression proportions were identical across the two marker types, although at the individual level,microsatellites’ performance was relatively poor at the upper range of Q-values, a result reflected by their lower pre-cision. Our results suggest that SNPs are more accurate and powerful than microsatellites for identification of A. m.mellifera colonies, especially when they are selected by information content. |
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SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera)Apis mellifera melliferaDark European honeybeeHoneybee conservationINtrogressionMicrosatellitesSNPsThe honeybee (Apis mellifera) has been threatened by multiple factors including pests and pathogens, pesticidesand loss of locally adapted gene complexes due to replacement and introgression. In western Europe, the geneticintegrity of the native A. m. mellifera (M-lineage) is endangered due to trading and intensive queen breeding withcommercial subspecies of eastern European ancestry (C-lineage). Effective conservation actions require reliablemolecular tools to identify pure-bred A. m. mellifera colonies. Microsatellites have been preferred for identificationof A. m. mellifera stocks across conservation centres. However, owing to high throughput, easy transferabilitybetween laboratories and low genotyping error, SNPs promise to become popular. Here, we compared the resolvingpower of a widely utilized microsatellite set to detect structure and introgression with that of different sets that com-bine a variable number of SNPs selected for their information content and genomic proximity to the microsatelliteloci. Contrary to every SNP data set, microsatellites did not discriminate between the two lineages in the PCA space.Mean introgression proportions were identical across the two marker types, although at the individual level,microsatellites’ performance was relatively poor at the upper range of Q-values, a result reflected by their lower pre-cision. Our results suggest that SNPs are more accurate and powerful than microsatellites for identification of A. m.mellifera colonies, especially when they are selected by information content.WileyBiblioteca Digital do IPBMuñoz, IreneHenriques, DoraJara, LauraJohnston, J. SpencerChávez-Galarza, JulioDe la Rúa, PilarPinto, M. Alice2017-09-25T16:46:10Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/14546engSNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera). Molecular Ecology Resources. ISSN 1755-098X. 17:4, p. 783-7951755-098X10.1111/1755-0998.12637info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:34:10Zoai:bibliotecadigital.ipb.pt:10198/14546Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:22.692926Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera) |
title |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera) |
spellingShingle |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera) Muñoz, Irene Apis mellifera mellifera Dark European honeybee Honeybee conservation INtrogression Microsatellites SNPs |
title_short |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera) |
title_full |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera) |
title_fullStr |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera) |
title_full_unstemmed |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera) |
title_sort |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera) |
author |
Muñoz, Irene |
author_facet |
Muñoz, Irene Henriques, Dora Jara, Laura Johnston, J. Spencer Chávez-Galarza, Julio De la Rúa, Pilar Pinto, M. Alice |
author_role |
author |
author2 |
Henriques, Dora Jara, Laura Johnston, J. Spencer Chávez-Galarza, Julio De la Rúa, Pilar Pinto, M. Alice |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Muñoz, Irene Henriques, Dora Jara, Laura Johnston, J. Spencer Chávez-Galarza, Julio De la Rúa, Pilar Pinto, M. Alice |
dc.subject.por.fl_str_mv |
Apis mellifera mellifera Dark European honeybee Honeybee conservation INtrogression Microsatellites SNPs |
topic |
Apis mellifera mellifera Dark European honeybee Honeybee conservation INtrogression Microsatellites SNPs |
description |
The honeybee (Apis mellifera) has been threatened by multiple factors including pests and pathogens, pesticidesand loss of locally adapted gene complexes due to replacement and introgression. In western Europe, the geneticintegrity of the native A. m. mellifera (M-lineage) is endangered due to trading and intensive queen breeding withcommercial subspecies of eastern European ancestry (C-lineage). Effective conservation actions require reliablemolecular tools to identify pure-bred A. m. mellifera colonies. Microsatellites have been preferred for identificationof A. m. mellifera stocks across conservation centres. However, owing to high throughput, easy transferabilitybetween laboratories and low genotyping error, SNPs promise to become popular. Here, we compared the resolvingpower of a widely utilized microsatellite set to detect structure and introgression with that of different sets that com-bine a variable number of SNPs selected for their information content and genomic proximity to the microsatelliteloci. Contrary to every SNP data set, microsatellites did not discriminate between the two lineages in the PCA space.Mean introgression proportions were identical across the two marker types, although at the individual level,microsatellites’ performance was relatively poor at the upper range of Q-values, a result reflected by their lower pre-cision. Our results suggest that SNPs are more accurate and powerful than microsatellites for identification of A. m.mellifera colonies, especially when they are selected by information content. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-25T16:46:10Z 2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/14546 |
url |
http://hdl.handle.net/10198/14546 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
SNPs selected by information content outperform randomly selected microsatellite loci for delineating genetic identification and introgression in the endangered dark European honeybee (Apis mellifera mellifera). Molecular Ecology Resources. ISSN 1755-098X. 17:4, p. 783-795 1755-098X 10.1111/1755-0998.12637 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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