Matrisome Profiling During Intervertebral Disc Development And Ageing
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/108375 https://doi.org/10.1038/s41598-017-11960-0 |
Resumo: | Intervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM profiling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, was performed to define the NP matrisome. The enrichment of Collagen XII and XIV in foetus, Fibronectin and Prolargin in elder NPs and Collagen XI in young ones was independently validated. This study provides the first matrisome database of healthy discs during development and ageing, which is key to determine the pathways and processes that maintain disc homeostasis. The factors identified may help to explain age-associated IVD degeneration or constitute putative effectors for disc regeneration. |
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Matrisome Profiling During Intervertebral Disc Development And AgeingAgingAnimalsCattleChromatography, LiquidComputational BiologyExtracellular MatrixExtracellular Matrix ProteinsIntervertebral DiscIntervertebral Disc DegenerationNucleus PulposusReproducibility of ResultsTandem Mass SpectrometryProteomeProteomicsIntervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM profiling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, was performed to define the NP matrisome. The enrichment of Collagen XII and XIV in foetus, Fibronectin and Prolargin in elder NPs and Collagen XI in young ones was independently validated. This study provides the first matrisome database of healthy discs during development and ageing, which is key to determine the pathways and processes that maintain disc homeostasis. The factors identified may help to explain age-associated IVD degeneration or constitute putative effectors for disc regeneration.Springer Nature2017-09-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108375http://hdl.handle.net/10316/108375https://doi.org/10.1038/s41598-017-11960-0engCaldeira, Joana B.Santa, CátiaOsório, HugoMolinos, MariaManadas, BrunoGonçalves, RaquelBarbosa, Márioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-28T08:17:49Zoai:estudogeral.uc.pt:10316/108375Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:40.535815Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Matrisome Profiling During Intervertebral Disc Development And Ageing |
title |
Matrisome Profiling During Intervertebral Disc Development And Ageing |
spellingShingle |
Matrisome Profiling During Intervertebral Disc Development And Ageing Caldeira, Joana B. Aging Animals Cattle Chromatography, Liquid Computational Biology Extracellular Matrix Extracellular Matrix Proteins Intervertebral Disc Intervertebral Disc Degeneration Nucleus Pulposus Reproducibility of Results Tandem Mass Spectrometry Proteome Proteomics |
title_short |
Matrisome Profiling During Intervertebral Disc Development And Ageing |
title_full |
Matrisome Profiling During Intervertebral Disc Development And Ageing |
title_fullStr |
Matrisome Profiling During Intervertebral Disc Development And Ageing |
title_full_unstemmed |
Matrisome Profiling During Intervertebral Disc Development And Ageing |
title_sort |
Matrisome Profiling During Intervertebral Disc Development And Ageing |
author |
Caldeira, Joana B. |
author_facet |
Caldeira, Joana B. Santa, Cátia Osório, Hugo Molinos, Maria Manadas, Bruno Gonçalves, Raquel Barbosa, Mário |
author_role |
author |
author2 |
Santa, Cátia Osório, Hugo Molinos, Maria Manadas, Bruno Gonçalves, Raquel Barbosa, Mário |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Caldeira, Joana B. Santa, Cátia Osório, Hugo Molinos, Maria Manadas, Bruno Gonçalves, Raquel Barbosa, Mário |
dc.subject.por.fl_str_mv |
Aging Animals Cattle Chromatography, Liquid Computational Biology Extracellular Matrix Extracellular Matrix Proteins Intervertebral Disc Intervertebral Disc Degeneration Nucleus Pulposus Reproducibility of Results Tandem Mass Spectrometry Proteome Proteomics |
topic |
Aging Animals Cattle Chromatography, Liquid Computational Biology Extracellular Matrix Extracellular Matrix Proteins Intervertebral Disc Intervertebral Disc Degeneration Nucleus Pulposus Reproducibility of Results Tandem Mass Spectrometry Proteome Proteomics |
description |
Intervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM profiling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, was performed to define the NP matrisome. The enrichment of Collagen XII and XIV in foetus, Fibronectin and Prolargin in elder NPs and Collagen XI in young ones was independently validated. This study provides the first matrisome database of healthy discs during development and ageing, which is key to determine the pathways and processes that maintain disc homeostasis. The factors identified may help to explain age-associated IVD degeneration or constitute putative effectors for disc regeneration. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/108375 http://hdl.handle.net/10316/108375 https://doi.org/10.1038/s41598-017-11960-0 |
url |
http://hdl.handle.net/10316/108375 https://doi.org/10.1038/s41598-017-11960-0 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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