Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/57041 |
Resumo: | Mannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal denaturation, hence a large number of biotechnological and clinical applications have been put forward. However, the current prohibitive production costs impose severe constraints towards large-scale applications. All known microbial producers synthesize MG from GDP-mannose and 3-phosphoglycerate via a two-step pathway in which mannosyl-3-phosphoglycerate is the intermediate metabolite. In an early work, this pathway was expressed in Saccharomyces cerevisiae with the goal to confirm gene function (Empadinhas et al. in J Bacteriol 186:4075--4084, 2004), but the level of MG accumulation was low. Therefore, in view of the potential biotechnological value of this compound, we decided to invest further effort to convert S. cerevisiae into an efficient cell factory for MG production. |
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Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimizationMannosylglycerateCompatible soluteGDP-mannoseYeast cell factoryMetabolic engineeringChemostat cultivationScience & TechnologyMannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal denaturation, hence a large number of biotechnological and clinical applications have been put forward. However, the current prohibitive production costs impose severe constraints towards large-scale applications. All known microbial producers synthesize MG from GDP-mannose and 3-phosphoglycerate via a two-step pathway in which mannosyl-3-phosphoglycerate is the intermediate metabolite. In an early work, this pathway was expressed in Saccharomyces cerevisiae with the goal to confirm gene function (Empadinhas et al. in J Bacteriol 186:4075--4084, 2004), but the level of MG accumulation was low. Therefore, in view of the potential biotechnological value of this compound, we decided to invest further effort to convert S. cerevisiae into an efficient cell factory for MG production.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte and also by project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI). Cristiana Faria was supported by a Ph.D. Grant from FCT (Ref. SFRH/ BD/79552/2011).info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoFaria, CristianaBorges, NunoRocha, I.Santos, Helena2018-11-162018-11-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/57041engFaria, Cristiana; Borges, Nuno; Rocha, Isabel; Santos, Helena, Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization. Microbial Cell Factories, 17(1), 178, 20181475-28591475-285910.1186/s12934-018-1023-730445960http://www.microbialcellfactories.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:35:03Zoai:repositorium.sdum.uminho.pt:1822/57041Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:30:51.372775Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization |
title |
Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization |
spellingShingle |
Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization Faria, Cristiana Mannosylglycerate Compatible solute GDP-mannose Yeast cell factory Metabolic engineering Chemostat cultivation Science & Technology |
title_short |
Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization |
title_full |
Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization |
title_fullStr |
Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization |
title_full_unstemmed |
Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization |
title_sort |
Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization |
author |
Faria, Cristiana |
author_facet |
Faria, Cristiana Borges, Nuno Rocha, I. Santos, Helena |
author_role |
author |
author2 |
Borges, Nuno Rocha, I. Santos, Helena |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Faria, Cristiana Borges, Nuno Rocha, I. Santos, Helena |
dc.subject.por.fl_str_mv |
Mannosylglycerate Compatible solute GDP-mannose Yeast cell factory Metabolic engineering Chemostat cultivation Science & Technology |
topic |
Mannosylglycerate Compatible solute GDP-mannose Yeast cell factory Metabolic engineering Chemostat cultivation Science & Technology |
description |
Mannosylglycerate (MG) is one of the most widespread compatible solutes among marine microorganisms adapted to hot environments. This ionic solute holds excellent ability to protect proteins against thermal denaturation, hence a large number of biotechnological and clinical applications have been put forward. However, the current prohibitive production costs impose severe constraints towards large-scale applications. All known microbial producers synthesize MG from GDP-mannose and 3-phosphoglycerate via a two-step pathway in which mannosyl-3-phosphoglycerate is the intermediate metabolite. In an early work, this pathway was expressed in Saccharomyces cerevisiae with the goal to confirm gene function (Empadinhas et al. in J Bacteriol 186:4075--4084, 2004), but the level of MG accumulation was low. Therefore, in view of the potential biotechnological value of this compound, we decided to invest further effort to convert S. cerevisiae into an efficient cell factory for MG production. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-16 2018-11-16T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/57041 |
url |
http://hdl.handle.net/1822/57041 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Faria, Cristiana; Borges, Nuno; Rocha, Isabel; Santos, Helena, Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization. Microbial Cell Factories, 17(1), 178, 2018 1475-2859 1475-2859 10.1186/s12934-018-1023-7 30445960 http://www.microbialcellfactories.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132814491254784 |