Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/153470 |
Resumo: | Osteoarthritis (OA) is one of the most common joint disorders in western populations, affecting millions of people worldwide and with a rising incidence as life expectancy continues to increase. Current therapies for OA management fail to halt the progressive degradation of articular cartilage, urging the need for more effective therapies to improve function and enhance quality of life. Through phage display technology, biopanning on a heterogenous chondrocyte population isolated from six different OA donors, and using a random 12-amino acid peptide phage library, a cell-binding peptide selective for human OA chondrocytes (GFQMISNNVYMR) is identified. A two-fold increase in fluorescence intensity is observed for OA chondrocytes, compared to normal chondrocytes, when cells were incubated with the identified peptide conjugated to a fluorescent label, being selectively internalized by OA cells. The identified peptide can be further modified and exploited for developing early diagnostic of OA and/or improve drug delivery to target cells through peptide-drug conjugates. |
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Phage Display Identified Peptide with Selectivity for Human Osteoarthritic ChondrocytesOsteoarthritisHuman chondrocytesPhage displayPeptidesTargeted therapyDiagnosticOsteoarthritis (OA) is one of the most common joint disorders in western populations, affecting millions of people worldwide and with a rising incidence as life expectancy continues to increase. Current therapies for OA management fail to halt the progressive degradation of articular cartilage, urging the need for more effective therapies to improve function and enhance quality of life. Through phage display technology, biopanning on a heterogenous chondrocyte population isolated from six different OA donors, and using a random 12-amino acid peptide phage library, a cell-binding peptide selective for human OA chondrocytes (GFQMISNNVYMR) is identified. A two-fold increase in fluorescence intensity is observed for OA chondrocytes, compared to normal chondrocytes, when cells were incubated with the identified peptide conjugated to a fluorescent label, being selectively internalized by OA cells. The identified peptide can be further modified and exploited for developing early diagnostic of OA and/or improve drug delivery to target cells through peptide-drug conjugates.Wiley2023-10-122023-10-12T00:00:00Z2023-10-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/153470eng2366-398710.1002/adtp.202300263Martins, IMCanadas, RFPereira, HAzeredo, JReis, RLOliveira, JMAzevedo, HSinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:36:09Zoai:repositorio-aberto.up.pt:10216/153470Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:27:34.472247Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes |
title |
Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes |
spellingShingle |
Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes Martins, IM Osteoarthritis Human chondrocytes Phage display Peptides Targeted therapy Diagnostic |
title_short |
Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes |
title_full |
Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes |
title_fullStr |
Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes |
title_full_unstemmed |
Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes |
title_sort |
Phage Display Identified Peptide with Selectivity for Human Osteoarthritic Chondrocytes |
author |
Martins, IM |
author_facet |
Martins, IM Canadas, RF Pereira, H Azeredo, J Reis, RL Oliveira, JM Azevedo, HS |
author_role |
author |
author2 |
Canadas, RF Pereira, H Azeredo, J Reis, RL Oliveira, JM Azevedo, HS |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Martins, IM Canadas, RF Pereira, H Azeredo, J Reis, RL Oliveira, JM Azevedo, HS |
dc.subject.por.fl_str_mv |
Osteoarthritis Human chondrocytes Phage display Peptides Targeted therapy Diagnostic |
topic |
Osteoarthritis Human chondrocytes Phage display Peptides Targeted therapy Diagnostic |
description |
Osteoarthritis (OA) is one of the most common joint disorders in western populations, affecting millions of people worldwide and with a rising incidence as life expectancy continues to increase. Current therapies for OA management fail to halt the progressive degradation of articular cartilage, urging the need for more effective therapies to improve function and enhance quality of life. Through phage display technology, biopanning on a heterogenous chondrocyte population isolated from six different OA donors, and using a random 12-amino acid peptide phage library, a cell-binding peptide selective for human OA chondrocytes (GFQMISNNVYMR) is identified. A two-fold increase in fluorescence intensity is observed for OA chondrocytes, compared to normal chondrocytes, when cells were incubated with the identified peptide conjugated to a fluorescent label, being selectively internalized by OA cells. The identified peptide can be further modified and exploited for developing early diagnostic of OA and/or improve drug delivery to target cells through peptide-drug conjugates. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-12 2023-10-12T00:00:00Z 2023-10-12T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/153470 |
url |
https://hdl.handle.net/10216/153470 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2366-3987 10.1002/adtp.202300263 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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