Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care

Detalhes bibliográficos
Autor(a) principal: Martins, Gabriela V.
Data de Publicação: 2017
Outros Autores: Tavares, Ana P.M., Fortunato, Elvira, Sales, M. Goreti F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/146939
Resumo: European Research Council (ERC) ERC/StG/3P's/GA311086 ; Portuguese Foundation for Science and Technology (FCT) - SFRH/BD/94159/2013
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spelling Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-CareGLASSY-CARBON ELECTRODEDNA-DAMAGESENSITIVE DETECTIONPARKINSONS-DISEASEFLOW IMMUNOSENSORURIC-ACIDGRAPHENESENSORSFILMCancerGeneralSDG 3 - Good Health and Well-beingEuropean Research Council (ERC) ERC/StG/3P's/GA311086 ; Portuguese Foundation for Science and Technology (FCT) - SFRH/BD/94159/2013This work presents a cost-effective, label-free in point-of-care (POC) biosensor for the sensitive detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG), the most abundant oxidative product of DNA, that may allow a premature assessment of cancer disease, thereby improving diagnosis, prognostics and survival rates. The device targets the direct detection of 8-OHdG by using for the first time a carbon-ink 3-electrode on a paper substrate coupled to Differential Pulse Voltammetry readings. This design was optimized by adding nanostructured carbon materials to the ink and the conducting polymer PEDOT, enhancing the electrocatalytic properties of the sensor towards 8-OHdG detection. Meanwhile, the ability of this oxidative stress biomarker to undertake an oxidation reaction enabled the development of the sensing electrochemical device without the need of chemical probes and long incubation periods. This paper-modified sensor presented high electrochemical performance on the oxidation of 8-OHdG with a wide linear range (50-1000 ng/ml) and a low detection limit (14.4 ng/ml). Thus, our results showed the development of a direct and facile sensor with good reproducibility, stability, sensitivity and more importantly, selectivity. The proposed carbon-based electrochemical sensor is a potential candidate to be miniaturized to small portable size, which make it applicable for in-situ 8-OHdG sensing in real biological samples.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisRUNMartins, Gabriela V.Tavares, Ana P.M.Fortunato, ElviraSales, M. Goreti F.2023-01-04T22:10:14Z2017-12-012017-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/146939eng2045-2322PURE: 3801539https://doi.org/10.1038/s41598-017-14878-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:27:53Zoai:run.unl.pt:10362/146939Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:42.920351Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care
title Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care
spellingShingle Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care
Martins, Gabriela V.
GLASSY-CARBON ELECTRODE
DNA-DAMAGE
SENSITIVE DETECTION
PARKINSONS-DISEASE
FLOW IMMUNOSENSOR
URIC-ACID
GRAPHENE
SENSORS
FILM
Cancer
General
SDG 3 - Good Health and Well-being
title_short Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care
title_full Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care
title_fullStr Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care
title_full_unstemmed Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care
title_sort Paper-Based Sensing Device for Electrochemical Detection of Oxidative Stress Biomarker 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Point-of-Care
author Martins, Gabriela V.
author_facet Martins, Gabriela V.
Tavares, Ana P.M.
Fortunato, Elvira
Sales, M. Goreti F.
author_role author
author2 Tavares, Ana P.M.
Fortunato, Elvira
Sales, M. Goreti F.
author2_role author
author
author
dc.contributor.none.fl_str_mv CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
RUN
dc.contributor.author.fl_str_mv Martins, Gabriela V.
Tavares, Ana P.M.
Fortunato, Elvira
Sales, M. Goreti F.
dc.subject.por.fl_str_mv GLASSY-CARBON ELECTRODE
DNA-DAMAGE
SENSITIVE DETECTION
PARKINSONS-DISEASE
FLOW IMMUNOSENSOR
URIC-ACID
GRAPHENE
SENSORS
FILM
Cancer
General
SDG 3 - Good Health and Well-being
topic GLASSY-CARBON ELECTRODE
DNA-DAMAGE
SENSITIVE DETECTION
PARKINSONS-DISEASE
FLOW IMMUNOSENSOR
URIC-ACID
GRAPHENE
SENSORS
FILM
Cancer
General
SDG 3 - Good Health and Well-being
description European Research Council (ERC) ERC/StG/3P's/GA311086 ; Portuguese Foundation for Science and Technology (FCT) - SFRH/BD/94159/2013
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
2017-12-01T00:00:00Z
2023-01-04T22:10:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/146939
url http://hdl.handle.net/10362/146939
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 3801539
https://doi.org/10.1038/s41598-017-14878-9
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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