EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774

Detalhes bibliográficos
Autor(a) principal: González, Pablo J.
Data de Publicação: 2006
Outros Autores: Rivas, Maria G., Brondino, Carlos D., Bursakov, Sergey A., Moura, Isabel, Moura, José J. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8706
Resumo: J Biol Inorg Chem (2006) 11: 609–616 DOI 10.1007/s00775-006-0110-0
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spelling EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774Molybdenum-containing enzymesPeriplasmic nitrate reductaseDimethyl sulfoxide reductase familyElectron paramagnetic resonanceRedox titrationJ Biol Inorg Chem (2006) 11: 609–616 DOI 10.1007/s00775-006-0110-0Nitrate reductases are enzymes that catalyze the conversion of nitrate to nitrite. We report here electron paramagnetic resonance (EPR) studies in the periplasmic nitrate reductase isolated from the sulfate-reducing bacteria Desulfovibrio desulfuricans ATCC 27774. This protein, belonging to the dimethyl sulfoxide reductase family of mononuclear Mo-containing enzymes, comprises a single 80-kDa subunit and contains a Mo bis(molybdopterin guanosine dinucleotide) cofactor and a [4Fe-4S] cluster. EPR-monitored redox titrations, carried out with and without nitrate in the potential range from 200 to -500 mV, and EPR studies of the enzyme, in both catalytic and inhibited conditions, reveal distinct types of Mo(V) EPR-active species, which indicates that the Mo site presents high coordination flexibility. These studies show that nitrate modulates the redox properties of the Mo active site, but not those of the [4Fe-4S] center. The possible structures and the role in catalysis of the distinct Mo(V) species detected by EPR are discussed.SpringerRUNGonzález, Pablo J.Rivas, Maria G.Brondino, Carlos D.Bursakov, Sergey A.Moura, IsabelMoura, José J. G.2013-02-06T10:53:21Z20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8706eng0949-8257info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:35Zoai:run.unl.pt:10362/8706Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:23.368744Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774
title EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774
spellingShingle EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774
González, Pablo J.
Molybdenum-containing enzymes
Periplasmic nitrate reductase
Dimethyl sulfoxide reductase family
Electron paramagnetic resonance
Redox titration
title_short EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774
title_full EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774
title_fullStr EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774
title_full_unstemmed EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774
title_sort EPR and redox properties of periplasmic nitrate reductase from Desulfovibrio desulfuricans ATCC 27774
author González, Pablo J.
author_facet González, Pablo J.
Rivas, Maria G.
Brondino, Carlos D.
Bursakov, Sergey A.
Moura, Isabel
Moura, José J. G.
author_role author
author2 Rivas, Maria G.
Brondino, Carlos D.
Bursakov, Sergey A.
Moura, Isabel
Moura, José J. G.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv González, Pablo J.
Rivas, Maria G.
Brondino, Carlos D.
Bursakov, Sergey A.
Moura, Isabel
Moura, José J. G.
dc.subject.por.fl_str_mv Molybdenum-containing enzymes
Periplasmic nitrate reductase
Dimethyl sulfoxide reductase family
Electron paramagnetic resonance
Redox titration
topic Molybdenum-containing enzymes
Periplasmic nitrate reductase
Dimethyl sulfoxide reductase family
Electron paramagnetic resonance
Redox titration
description J Biol Inorg Chem (2006) 11: 609–616 DOI 10.1007/s00775-006-0110-0
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
2013-02-06T10:53:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8706
url http://hdl.handle.net/10362/8706
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0949-8257
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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