Differentiation and gene flow among European populations of Leishmania infantum MON-1
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/117211 |
Resumo: | BACKGROUND: Leishmania infantum is the causative agent of visceral and cutaneous leishmaniasis in the Mediterranean region, South America, and China. MON-1 L. infantum is the predominating zymodeme in all endemic regions, both in humans and dogs, the reservoir host. In order to answer important epidemiological questions it is essential to discriminate strains of MON-1. METHODOLOGY/PRINCIPAL FINDINGS: We have used a set of 14 microsatellite markers to analyse 141 strains of L. infantum mainly from Spain, Portugal, and Greece of which 107 strains were typed by MLEE as MON-1. The highly variable microsatellites have the potential to discriminate MON-1 strains from other L. infantum zymodemes and even within MON-1 strains. Model- and distance-based analysis detected a considerable amount of structure within European L. infantum. Two major monophyletic groups-MON-1 and non-MON-1-could be distinguished, with non-MON-1 being more polymorphic. Strains of MON-98, 77, and 108 were always part of the MON-1 group. Among MON-1, three geographically determined and genetically differentiated populations could be identified: (1) Greece; (2) Spain islands-Majorca/Ibiza; (3) mainland Portugal/Spain. All four populations showed a predominantly clonal structure; however, there are indications of occasional recombination events and gene flow even between MON-1 and non-MON-1. Sand fly vectors seem to play an important role in sustaining genetic diversity. No correlation was observed between Leishmania genotypes, host specificity, and clinical manifestation. In the case of relapse/re-infection, only re-infections by a strain with a different MLMT profile can be unequivocally identified, since not all strains have individual MLMT profiles. CONCLUSION: In the present study for the first time several key epidemiological questions could be addressed for the MON-1 zymodeme, because of the high discriminatory power of microsatellite markers, thus creating a basis for further epidemiological investigations. |
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Differentiation and gene flow among European populations of Leishmania infantum MON-1AnimalsDog DiseasesDogsEuropeGene FlowGenetic VariationGenotypeHost-Parasite InteractionsHumansLeishmania infantumLeishmaniasis, VisceralMicrosatellite RepeatsPhylogenyJournal ArticleResearch Support, Non-U.S. Gov'tParasitologyGeneticsEcology, Evolution, Behavior and SystematicsSDG 3 - Good Health and Well-beingBACKGROUND: Leishmania infantum is the causative agent of visceral and cutaneous leishmaniasis in the Mediterranean region, South America, and China. MON-1 L. infantum is the predominating zymodeme in all endemic regions, both in humans and dogs, the reservoir host. In order to answer important epidemiological questions it is essential to discriminate strains of MON-1. METHODOLOGY/PRINCIPAL FINDINGS: We have used a set of 14 microsatellite markers to analyse 141 strains of L. infantum mainly from Spain, Portugal, and Greece of which 107 strains were typed by MLEE as MON-1. The highly variable microsatellites have the potential to discriminate MON-1 strains from other L. infantum zymodemes and even within MON-1 strains. Model- and distance-based analysis detected a considerable amount of structure within European L. infantum. Two major monophyletic groups-MON-1 and non-MON-1-could be distinguished, with non-MON-1 being more polymorphic. Strains of MON-98, 77, and 108 were always part of the MON-1 group. Among MON-1, three geographically determined and genetically differentiated populations could be identified: (1) Greece; (2) Spain islands-Majorca/Ibiza; (3) mainland Portugal/Spain. All four populations showed a predominantly clonal structure; however, there are indications of occasional recombination events and gene flow even between MON-1 and non-MON-1. Sand fly vectors seem to play an important role in sustaining genetic diversity. No correlation was observed between Leishmania genotypes, host specificity, and clinical manifestation. In the case of relapse/re-infection, only re-infections by a strain with a different MLMT profile can be unequivocally identified, since not all strains have individual MLMT profiles. CONCLUSION: In the present study for the first time several key epidemiological questions could be addressed for the MON-1 zymodeme, because of the high discriminatory power of microsatellite markers, thus creating a basis for further epidemiological investigations.Instituto de Higiene e Medicina Tropical (IHMT)Centro de Malária e outras Doenças Tropicais (CMDT)Unidade de Parasitologia e Microbiologia Médicas (UPMM)RUNKuhls, KatrinChicharro, CarmenCañavate, CarmenCortes, SofiaCampino, LeneaHaralambous, ChristosSoteriadou, KettyPratlong, FrancineDedet, Jean-PierreMauricio, IsabelMiles, MichaelSchaar, MatthiasOchsenreither, SebastianRadtke, Oliver ASchönian, Gabriele2021-05-06T22:39:44Z2008-07-092008-07-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://hdl.handle.net/10362/117211eng1935-2727PURE: 2378414https://doi.org/10.1371/journal.pntd.0000261info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:00:18Zoai:run.unl.pt:10362/117211Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:32.185861Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differentiation and gene flow among European populations of Leishmania infantum MON-1 |
title |
Differentiation and gene flow among European populations of Leishmania infantum MON-1 |
spellingShingle |
Differentiation and gene flow among European populations of Leishmania infantum MON-1 Kuhls, Katrin Animals Dog Diseases Dogs Europe Gene Flow Genetic Variation Genotype Host-Parasite Interactions Humans Leishmania infantum Leishmaniasis, Visceral Microsatellite Repeats Phylogeny Journal Article Research Support, Non-U.S. Gov't Parasitology Genetics Ecology, Evolution, Behavior and Systematics SDG 3 - Good Health and Well-being |
title_short |
Differentiation and gene flow among European populations of Leishmania infantum MON-1 |
title_full |
Differentiation and gene flow among European populations of Leishmania infantum MON-1 |
title_fullStr |
Differentiation and gene flow among European populations of Leishmania infantum MON-1 |
title_full_unstemmed |
Differentiation and gene flow among European populations of Leishmania infantum MON-1 |
title_sort |
Differentiation and gene flow among European populations of Leishmania infantum MON-1 |
author |
Kuhls, Katrin |
author_facet |
Kuhls, Katrin Chicharro, Carmen Cañavate, Carmen Cortes, Sofia Campino, Lenea Haralambous, Christos Soteriadou, Ketty Pratlong, Francine Dedet, Jean-Pierre Mauricio, Isabel Miles, Michael Schaar, Matthias Ochsenreither, Sebastian Radtke, Oliver A Schönian, Gabriele |
author_role |
author |
author2 |
Chicharro, Carmen Cañavate, Carmen Cortes, Sofia Campino, Lenea Haralambous, Christos Soteriadou, Ketty Pratlong, Francine Dedet, Jean-Pierre Mauricio, Isabel Miles, Michael Schaar, Matthias Ochsenreither, Sebastian Radtke, Oliver A Schönian, Gabriele |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) Unidade de Parasitologia e Microbiologia Médicas (UPMM) RUN |
dc.contributor.author.fl_str_mv |
Kuhls, Katrin Chicharro, Carmen Cañavate, Carmen Cortes, Sofia Campino, Lenea Haralambous, Christos Soteriadou, Ketty Pratlong, Francine Dedet, Jean-Pierre Mauricio, Isabel Miles, Michael Schaar, Matthias Ochsenreither, Sebastian Radtke, Oliver A Schönian, Gabriele |
dc.subject.por.fl_str_mv |
Animals Dog Diseases Dogs Europe Gene Flow Genetic Variation Genotype Host-Parasite Interactions Humans Leishmania infantum Leishmaniasis, Visceral Microsatellite Repeats Phylogeny Journal Article Research Support, Non-U.S. Gov't Parasitology Genetics Ecology, Evolution, Behavior and Systematics SDG 3 - Good Health and Well-being |
topic |
Animals Dog Diseases Dogs Europe Gene Flow Genetic Variation Genotype Host-Parasite Interactions Humans Leishmania infantum Leishmaniasis, Visceral Microsatellite Repeats Phylogeny Journal Article Research Support, Non-U.S. Gov't Parasitology Genetics Ecology, Evolution, Behavior and Systematics SDG 3 - Good Health and Well-being |
description |
BACKGROUND: Leishmania infantum is the causative agent of visceral and cutaneous leishmaniasis in the Mediterranean region, South America, and China. MON-1 L. infantum is the predominating zymodeme in all endemic regions, both in humans and dogs, the reservoir host. In order to answer important epidemiological questions it is essential to discriminate strains of MON-1. METHODOLOGY/PRINCIPAL FINDINGS: We have used a set of 14 microsatellite markers to analyse 141 strains of L. infantum mainly from Spain, Portugal, and Greece of which 107 strains were typed by MLEE as MON-1. The highly variable microsatellites have the potential to discriminate MON-1 strains from other L. infantum zymodemes and even within MON-1 strains. Model- and distance-based analysis detected a considerable amount of structure within European L. infantum. Two major monophyletic groups-MON-1 and non-MON-1-could be distinguished, with non-MON-1 being more polymorphic. Strains of MON-98, 77, and 108 were always part of the MON-1 group. Among MON-1, three geographically determined and genetically differentiated populations could be identified: (1) Greece; (2) Spain islands-Majorca/Ibiza; (3) mainland Portugal/Spain. All four populations showed a predominantly clonal structure; however, there are indications of occasional recombination events and gene flow even between MON-1 and non-MON-1. Sand fly vectors seem to play an important role in sustaining genetic diversity. No correlation was observed between Leishmania genotypes, host specificity, and clinical manifestation. In the case of relapse/re-infection, only re-infections by a strain with a different MLMT profile can be unequivocally identified, since not all strains have individual MLMT profiles. CONCLUSION: In the present study for the first time several key epidemiological questions could be addressed for the MON-1 zymodeme, because of the high discriminatory power of microsatellite markers, thus creating a basis for further epidemiological investigations. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-07-09 2008-07-09T00:00:00Z 2021-05-06T22:39:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/117211 |
url |
http://hdl.handle.net/10362/117211 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1935-2727 PURE: 2378414 https://doi.org/10.1371/journal.pntd.0000261 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
18 application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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