Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.

Detalhes bibliográficos
Autor(a) principal: Gutiérrez-Gutiérrez, Carlos
Data de Publicação: 2017
Outros Autores: Santos, Margarida, Mota, Manuel
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/28180
Resumo: “Needle” (Longidorus spp.) nematodes are polyphagous root ectoparasites causing severe damage to plants by their direct feeding, and in addition some species can transmit plant viruses. In order to establish the species diversity of the genus Longidorus in central-Western Portugal and its distribution, we sampled several vineyards in the Lisbon wine regions from 2015 to 2017 during the spring and autumn.. An integrative taxonomic approach based on the combination of morphometric and morphological characterizations with molecular analysis using ribosomal DNA (rDNA) sequences from ITS regions and D2–D3 expansion segments of the 28S gene were used for species delimitation and identification. As results, two needle species morphologically similar, L. vinearum and L. vineacola, were found parasitizing the rhizosphere of grapevine (Vitis vinifera L.). In this study, L. vinearum is the most frequently found species.
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spelling Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.LongidoridaerDNAD2-D3ITS1virus vector nematodes“Needle” (Longidorus spp.) nematodes are polyphagous root ectoparasites causing severe damage to plants by their direct feeding, and in addition some species can transmit plant viruses. In order to establish the species diversity of the genus Longidorus in central-Western Portugal and its distribution, we sampled several vineyards in the Lisbon wine regions from 2015 to 2017 during the spring and autumn.. An integrative taxonomic approach based on the combination of morphometric and morphological characterizations with molecular analysis using ribosomal DNA (rDNA) sequences from ITS regions and D2–D3 expansion segments of the 28S gene were used for species delimitation and identification. As results, two needle species morphologically similar, L. vinearum and L. vineacola, were found parasitizing the rhizosphere of grapevine (Vitis vinifera L.). In this study, L. vinearum is the most frequently found species.Sociedade Portuguesa de Fitopatologia - SPF.2020-10-23T11:01:02Z2020-10-232017-10-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/28180http://hdl.handle.net/10174/28180enghttp://spfitopatologia.pt/index.php/congressos-spf/item/74-8-congresso-spf-2017naonaosimcarlosg@uevora.ptndnd581https://drive.google.com/file/d/0B54NeQVshkyacmxQQ3ZIam1Uams/viewGutiérrez-Gutiérrez, CarlosSantos, MargaridaMota, Manuelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:24:25Zoai:dspace.uevora.pt:10174/28180Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:18:10.148930Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
title Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
spellingShingle Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
Gutiérrez-Gutiérrez, Carlos
Longidoridae
rDNA
D2-D3
ITS1
virus vector nematodes
title_short Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
title_full Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
title_fullStr Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
title_full_unstemmed Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
title_sort Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
author Gutiérrez-Gutiérrez, Carlos
author_facet Gutiérrez-Gutiérrez, Carlos
Santos, Margarida
Mota, Manuel
author_role author
author2 Santos, Margarida
Mota, Manuel
author2_role author
author
dc.contributor.author.fl_str_mv Gutiérrez-Gutiérrez, Carlos
Santos, Margarida
Mota, Manuel
dc.subject.por.fl_str_mv Longidoridae
rDNA
D2-D3
ITS1
virus vector nematodes
topic Longidoridae
rDNA
D2-D3
ITS1
virus vector nematodes
description “Needle” (Longidorus spp.) nematodes are polyphagous root ectoparasites causing severe damage to plants by their direct feeding, and in addition some species can transmit plant viruses. In order to establish the species diversity of the genus Longidorus in central-Western Portugal and its distribution, we sampled several vineyards in the Lisbon wine regions from 2015 to 2017 during the spring and autumn.. An integrative taxonomic approach based on the combination of morphometric and morphological characterizations with molecular analysis using ribosomal DNA (rDNA) sequences from ITS regions and D2–D3 expansion segments of the 28S gene were used for species delimitation and identification. As results, two needle species morphologically similar, L. vinearum and L. vineacola, were found parasitizing the rhizosphere of grapevine (Vitis vinifera L.). In this study, L. vinearum is the most frequently found species.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-27T00:00:00Z
2020-10-23T11:01:02Z
2020-10-23
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/28180
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://spfitopatologia.pt/index.php/congressos-spf/item/74-8-congresso-spf-2017
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carlosg@uevora.pt
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https://drive.google.com/file/d/0B54NeQVshkyacmxQQ3ZIam1Uams/view
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dc.publisher.none.fl_str_mv Sociedade Portuguesa de Fitopatologia - SPF.
publisher.none.fl_str_mv Sociedade Portuguesa de Fitopatologia - SPF.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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