Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/28180 |
Resumo: | “Needle” (Longidorus spp.) nematodes are polyphagous root ectoparasites causing severe damage to plants by their direct feeding, and in addition some species can transmit plant viruses. In order to establish the species diversity of the genus Longidorus in central-Western Portugal and its distribution, we sampled several vineyards in the Lisbon wine regions from 2015 to 2017 during the spring and autumn.. An integrative taxonomic approach based on the combination of morphometric and morphological characterizations with molecular analysis using ribosomal DNA (rDNA) sequences from ITS regions and D2–D3 expansion segments of the 28S gene were used for species delimitation and identification. As results, two needle species morphologically similar, L. vinearum and L. vineacola, were found parasitizing the rhizosphere of grapevine (Vitis vinifera L.). In this study, L. vinearum is the most frequently found species. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal.LongidoridaerDNAD2-D3ITS1virus vector nematodes“Needle” (Longidorus spp.) nematodes are polyphagous root ectoparasites causing severe damage to plants by their direct feeding, and in addition some species can transmit plant viruses. In order to establish the species diversity of the genus Longidorus in central-Western Portugal and its distribution, we sampled several vineyards in the Lisbon wine regions from 2015 to 2017 during the spring and autumn.. An integrative taxonomic approach based on the combination of morphometric and morphological characterizations with molecular analysis using ribosomal DNA (rDNA) sequences from ITS regions and D2–D3 expansion segments of the 28S gene were used for species delimitation and identification. As results, two needle species morphologically similar, L. vinearum and L. vineacola, were found parasitizing the rhizosphere of grapevine (Vitis vinifera L.). In this study, L. vinearum is the most frequently found species.Sociedade Portuguesa de Fitopatologia - SPF.2020-10-23T11:01:02Z2020-10-232017-10-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/28180http://hdl.handle.net/10174/28180enghttp://spfitopatologia.pt/index.php/congressos-spf/item/74-8-congresso-spf-2017naonaosimcarlosg@uevora.ptndnd581https://drive.google.com/file/d/0B54NeQVshkyacmxQQ3ZIam1Uams/viewGutiérrez-Gutiérrez, CarlosSantos, MargaridaMota, Manuelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:24:25Zoai:dspace.uevora.pt:10174/28180Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:18:10.148930Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal. |
title |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal. |
spellingShingle |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal. Gutiérrez-Gutiérrez, Carlos Longidoridae rDNA D2-D3 ITS1 virus vector nematodes |
title_short |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal. |
title_full |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal. |
title_fullStr |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal. |
title_full_unstemmed |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal. |
title_sort |
Integrative taxonomic approach and molecular phylogeny for identification of needle nematode species infesting grapevine soils in Portugal. |
author |
Gutiérrez-Gutiérrez, Carlos |
author_facet |
Gutiérrez-Gutiérrez, Carlos Santos, Margarida Mota, Manuel |
author_role |
author |
author2 |
Santos, Margarida Mota, Manuel |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Gutiérrez-Gutiérrez, Carlos Santos, Margarida Mota, Manuel |
dc.subject.por.fl_str_mv |
Longidoridae rDNA D2-D3 ITS1 virus vector nematodes |
topic |
Longidoridae rDNA D2-D3 ITS1 virus vector nematodes |
description |
“Needle” (Longidorus spp.) nematodes are polyphagous root ectoparasites causing severe damage to plants by their direct feeding, and in addition some species can transmit plant viruses. In order to establish the species diversity of the genus Longidorus in central-Western Portugal and its distribution, we sampled several vineyards in the Lisbon wine regions from 2015 to 2017 during the spring and autumn.. An integrative taxonomic approach based on the combination of morphometric and morphological characterizations with molecular analysis using ribosomal DNA (rDNA) sequences from ITS regions and D2–D3 expansion segments of the 28S gene were used for species delimitation and identification. As results, two needle species morphologically similar, L. vinearum and L. vineacola, were found parasitizing the rhizosphere of grapevine (Vitis vinifera L.). In this study, L. vinearum is the most frequently found species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-27T00:00:00Z 2020-10-23T11:01:02Z 2020-10-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/28180 http://hdl.handle.net/10174/28180 |
url |
http://hdl.handle.net/10174/28180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://spfitopatologia.pt/index.php/congressos-spf/item/74-8-congresso-spf-2017 nao nao sim carlosg@uevora.pt nd nd 581 https://drive.google.com/file/d/0B54NeQVshkyacmxQQ3ZIam1Uams/view |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Fitopatologia - SPF. |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Fitopatologia - SPF. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799136663520149504 |