Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme

Detalhes bibliográficos
Autor(a) principal: Ziane, Hanifa
Data de Publicação: 2015
Outros Autores: Manageiro, Vera, Ferreira, Eugénia, Bektache, Soumia, Tazir, Mohamed, Caniça, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/3730
Resumo: We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.
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spelling Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based schemeMultiplex PCRPneumococciQuellung ReactionSensitivitySpecificitySerogroup/typeResistência aos AntimicrobianosWe developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.H. Ziane was supported by a grant from Ministère de l'Enseignement Superieur et de la Recherche Scientifique in Algeria. V. Manageiro was supported by the grant SFRH/BPD/77486/2011, from Fundação para a Ciência e a Tecnologia, Lisbon, Portugal.ElsevierRepositório Científico do Instituto Nacional de SaúdeZiane, HanifaManageiro, VeraFerreira, EugéniaBektache, SoumiaTazir, MohamedCaniça, Manuela2020-12-01T01:30:10Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3730engJ Microbiol Methods. 2015 Dec;119:243-6. doi: 10.1016/j.mimet.2015.11.002. Epub 2015 Nov 4.0167-701210.1016/j.mimet.2015.11.002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:57Zoai:repositorio.insa.pt:10400.18/3730Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:34.339080Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
title Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
spellingShingle Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
Ziane, Hanifa
Multiplex PCR
Pneumococci
Quellung Reaction
Sensitivity
Specificity
Serogroup/type
Resistência aos Antimicrobianos
title_short Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
title_full Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
title_fullStr Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
title_full_unstemmed Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
title_sort Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme
author Ziane, Hanifa
author_facet Ziane, Hanifa
Manageiro, Vera
Ferreira, Eugénia
Bektache, Soumia
Tazir, Mohamed
Caniça, Manuela
author_role author
author2 Manageiro, Vera
Ferreira, Eugénia
Bektache, Soumia
Tazir, Mohamed
Caniça, Manuela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Ziane, Hanifa
Manageiro, Vera
Ferreira, Eugénia
Bektache, Soumia
Tazir, Mohamed
Caniça, Manuela
dc.subject.por.fl_str_mv Multiplex PCR
Pneumococci
Quellung Reaction
Sensitivity
Specificity
Serogroup/type
Resistência aos Antimicrobianos
topic Multiplex PCR
Pneumococci
Quellung Reaction
Sensitivity
Specificity
Serogroup/type
Resistência aos Antimicrobianos
description We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2020-12-01T01:30:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/3730
url http://hdl.handle.net/10400.18/3730
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Microbiol Methods. 2015 Dec;119:243-6. doi: 10.1016/j.mimet.2015.11.002. Epub 2015 Nov 4.
0167-7012
10.1016/j.mimet.2015.11.002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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